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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000242                                                              18    1135
XP_005264389                                                           18    1135
NP_055838                                                              17    3301
NP_001263181                                                           17    3301
NP_056099                                                              16    5631
NP_005688                                                              16    5631
XP_006720432                                                           16    5631
XP_006710565                                                           16    5631
XP_006710564                                                           16    5631
XP_005270743                                                           16    5631
NP_001135758                                                           16    7355
NP_078900                                                              16    7355
XP_006712166                                                           16    7355
XP_005262685                                                           16    7355
NP_000278                                                              15    9605
NP_619639                                                              15    9605
NP_733842                                                              15    9605
NP_001007526                                                           15    9605
NP_001277284                                                           15    9605
XP_005270206                                                           15    9605
XP_005249237                                                           15    9605

>NP_000242
          Length = 934

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SAAEVGLVR 9
          SAAEVG VR
Sbjct: 13 SAAEVGFVR 21



>XP_005264389
          Length = 921

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SAAEVGLVR 9
          SAAEVG VR
Sbjct: 13 SAAEVGFVR 21



>NP_055838
          Length = 676

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAAEVGLVR 9
           SAAE GL+R
Sbjct: 152 SAAEDGLIR 160



>NP_001263181
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAAEVGLVR 9
           SAAE GL+R
Sbjct: 152 SAAEDGLIR 160



>NP_056099
          Length = 3375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AAEVGLVR 9
            AAEVG+ R
Sbjct: 3195 AAEVGMAR 3202



>NP_005688
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SAAEVGLVR 9
          SAA+ GL+R
Sbjct: 79 SAAQAGLLR 87



>XP_006720432
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SAAEVGLVR 9
           SAA+ GL+R
Sbjct: 122 SAAQAGLLR 130



>XP_006710565
          Length = 3140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AAEVGLVR 9
            AAEVG+ R
Sbjct: 2960 AAEVGMAR 2967



>XP_006710564
          Length = 3432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AAEVGLVR 9
            AAEVG+ R
Sbjct: 3252 AAEVGMAR 3259



>XP_005270743
          Length = 3453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AAEVGLVR 9
            AAEVG+ R
Sbjct: 3273 AAEVGMAR 3280



>NP_001135758
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SAAEVGLV 8
          +AAEVG++
Sbjct: 41 TAAEVGII 48



>NP_078900
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SAAEVGLV 8
          +AAEVG++
Sbjct: 41 TAAEVGII 48



>XP_006712166
          Length = 976

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SAAEVGLV 8
          +AAEVG++
Sbjct: 63 TAAEVGII 70



>XP_005262685
          Length = 1113

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SAAEVGLV 8
          +AAEVG++
Sbjct: 63 TAAEVGII 70



>NP_000278
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAEVGLV 8
          AAE+GLV
Sbjct: 31 AAELGLV 37



>NP_619639
          Length = 4074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AAEVGLV 8
            AAEVGL+
Sbjct: 2947 AAEVGLL 2953



>NP_733842
          Length = 3396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AAEVGLV 8
            AAEVGL+
Sbjct: 2947 AAEVGLL 2953



>NP_001007526
          Length = 1432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVGLVR 9
           EVGLVR
Sbjct: 783 EVGLVR 788



>NP_001277284
          Length = 1429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVGLVR 9
           EVGLVR
Sbjct: 648 EVGLVR 653



>XP_005270206
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SAAEVGLVR 9
          +A E GLVR
Sbjct: 61 AAKEAGLVR 69



>XP_005249237
          Length = 892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  AAEVGLV 8
          AAE+GLV
Sbjct: 31 AAELGLV 37



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.127    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 348,981
Number of extensions: 303
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)