Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
XP_006715635                                                           17    2528
NP_114155                                                              17    2528
NP_060034                                                              17    4312
NP_001164225                                                           17    4312
XP_006719539                                                           17    4312
XP_006710440                                                           17    4312
NP_000172                                                              16    5631
NP_689585                                                              16    5631
NP_005415                                                              16    5631
NP_001240286                                                           16    5631
XP_005250354                                                           16    5631
XP_005248296                                                           16    5631
XP_005248295                                                           16    5631
XP_006710932                                                           16    5631
XP_005271221                                                           16    5631
XP_005271220                                                           16    5631
NP_009208                                                              16    5631
NP_078962                                                              16    7355
NP_001177874                                                           16    7355
NP_001177876                                                           16    7355
NP_005649                                                              16    7355
NP_001275664                                                           16    7355
NP_001275665                                                           16    7355
NP_008972                                                              16    7355
NP_001104582                                                           16    7355
NP_005330                                                              16    7355
NP_997245                                                              16    7355
XP_005269517                                                           16    7355
XP_005263984                                                           16    7355
XP_005263983                                                           16    7355
XP_005263982                                                           16    7355
XP_005263981                                                           16    7355
XP_005263980                                                           16    7355
XP_006710966                                                           16    7355
XP_005271267                                                           16    7355
XP_005271266                                                           16    7355
XP_005271265                                                           16    7355
NP_001129496                                                           15    9605
NP_938011                                                              15    9605
NP_056046                                                              15    9605
NP_001026871                                                           15    9605
XP_005274596                                                           15    9605
XP_006718233                                                           15    9605
XP_006716625                                                           15    9605
>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RYKLEVRLY 9
           RYKL VRLY
Sbjct: 445 RYKLGVRLY 453
>XP_006715635
          Length = 519

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RYKLEVRLY 9
           RYK ++R+Y
Sbjct: 178 RYKCDLRIY 186
>NP_114155
          Length = 592

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RYKLEVRLY 9
           RYK ++R+Y
Sbjct: 251 RYKCDLRIY 259
>NP_060034
          Length = 2551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RYKLEVRLY 9
          RY  EVR Y
Sbjct: 72 RYTFEVRTY 80
>NP_001164225
          Length = 823

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+LE+R++
Sbjct: 128 YRLEIRIF 135
>XP_006719539
          Length = 2246

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RYKLEVRLY 9
          RY  EVR Y
Sbjct: 72 RYTFEVRTY 80
>XP_006710440
          Length = 792

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+LE+R++
Sbjct: 123 YRLEIRIF 130
>NP_000172
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 256 FKLEVRL 262
>NP_689585
          Length = 1437

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    RYKLEVR 7
            RY++EVR
Sbjct: 1380 RYRMEVR 1386
>NP_005415
          Length = 1138

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+L+V+LY
Sbjct: 616 YQLDVQLY 623
>NP_001240286
          Length = 1093

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+L+V+LY
Sbjct: 571 YQLDVQLY 578
>XP_005250354
          Length = 600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 256 FKLEVRL 262
>XP_005248296
          Length = 630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RYKLEVRLY 9
           RYK E +LY
Sbjct: 349 RYKDEAQLY 357
>XP_005248295
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RYKLEVRLY 9
           RYK E +LY
Sbjct: 464 RYKDEAQLY 472
>XP_006710932
          Length = 783

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+L+V+LY
Sbjct: 261 YQLDVQLY 268
>XP_005271221
          Length = 830

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+L+V+LY
Sbjct: 616 YQLDVQLY 623
>XP_005271220
          Length = 1095

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YKLEVRLY 9
           Y+L+V+LY
Sbjct: 573 YQLDVQLY 580
>NP_009208
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RYKLEVRLY 9
           RYK E +LY
Sbjct: 464 RYKDEAQLY 472
>NP_078962
          Length = 887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YK ++R+Y
Sbjct: 200 YKFDLRIY 207
>NP_001177874
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YKL +RLY
Sbjct: 303 YKLCLRLY 310
>NP_001177876
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YKL +RLY
Sbjct: 181 YKLCLRLY 188
>NP_005649
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YKL +RLY
Sbjct: 303 YKLCLRLY 310
>NP_001275664
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 172 HKLEVRL 178
>NP_001275665
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 261 HKLEVRL 267
>NP_008972
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 268 HKLEVRL 274
>NP_001104582
          Length = 157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  RYKLEVRL 8
          RY+LE+++
Sbjct: 55 RYQLEIKI 62
>NP_005330
          Length = 200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  RYKLEVRL 8
          RY+LE+++
Sbjct: 55 RYQLEIKI 62
>NP_997245
          Length = 962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 708 RYSAEVRL 715
>XP_005269517
          Length = 535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   YKLEVRL 8
           +KLEVRL
Sbjct: 285 HKLEVRL 291
>XP_005263984
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 708 RYSAEVRL 715
>XP_005263983
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 708 RYSAEVRL 715
>XP_005263982
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 722 RYSAEVRL 729
>XP_005263981
          Length = 990

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 736 RYSAEVRL 743
>XP_005263980
          Length = 991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RYKLEVRL 8
           RY  EVRL
Sbjct: 736 RYSAEVRL 743
>XP_006710966
          Length = 792

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YK ++R+Y
Sbjct: 200 YKFDLRIY 207
>XP_005271267
          Length = 798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YK ++R+Y
Sbjct: 111 YKFDLRIY 118
>XP_005271266
          Length = 798

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YK ++R+Y
Sbjct: 111 YKFDLRIY 118
>XP_005271265
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   YKLEVRLY 9
           YK ++R+Y
Sbjct: 173 YKFDLRIY 180
>NP_001129496
          Length = 1474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RYKLEVRLY 9
            RY +  RLY
Sbjct: 1437 RYSVRCRLY 1445
>NP_938011
          Length = 1630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RYKLEVRLY 9
            RY +  RLY
Sbjct: 1593 RYSVRCRLY 1601
>NP_056046
          Length = 1436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RYKLEVRLY 9
            RYK++  LY
Sbjct: 1298 RYKVQNNLY 1306
>NP_001026871
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   RYKLEVRLY 9
           RY  E+R Y
Sbjct: 131 RYPAEIRKY 139
>XP_005274596
          Length = 1651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RYKLEVRLY 9
            RY +  RLY
Sbjct: 1614 RYSVRCRLY 1622
>XP_006718233
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RYKLEVRLY 9
           RYK++  LY
Sbjct: 644 RYKVQNNLY 652
>XP_006716625
          Length = 369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  KLEVRLY 9
          KLE RLY
Sbjct: 65 KLEERLY 71
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.152    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 473,260
Number of extensions: 848
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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