Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001172003                                                           17    4312
NP_001171988                                                           17    4312
NP_078893                                                              17    4312
NP_006505                                                              17    4312
XP_005252265                                                           17    4312
XP_005252264                                                           17    4312
XP_006717348                                                           17    4312
XP_006717347                                                           17    4312
XP_006717346                                                           17    4312
XP_005265428                                                           17    4312
NP_667338                                                              16    7355
NP_001159608                                                           16    7355
NP_001888                                                              16    7355
NP_001268885                                                           16    7355
NP_443128                                                              16    7355
XP_006713923                                                           16    7355
XP_006713922                                                           16    7355
XP_006712934                                                           16    7355
XP_006712933                                                           16    7355
NP_852118                                                              16    7355
NP_036252                                                              15    9605
NP_443200                                                              15    9605
NP_001160070                                                           15    9605
NP_055698                                                              15    9605
NP_001015045                                                           15    9605
NP_001252509                                                           15    9605
NP_001252508                                                           15    9605
NP_001252507                                                           15    9605
NP_001001998                                                           15    9605
XP_005262351                                                           15    9605
XP_006718824                                                           15    9605
XP_005249034                                                           15    9605
XP_005249033                                                           15    9605
XP_005262742                                                           15    9605
XP_005262741                                                           15    9605
XP_006714120                                                           15    9605
XP_005262740                                                           15    9605
XP_005262739                                                           15    9605
XP_005262738                                                           15    9605
XP_005263532                                                           15    9605
NP_002676                                                              15    9605
>NP_001172003
          Length = 1259

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RTLSAYSA 8
           R LSAYSA
Sbjct: 896 RLLSAYSA 903
>NP_001171988
          Length = 1495

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>NP_078893
          Length = 1495

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>NP_006505
          Length = 1956

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTLSAYSAT 9
           RT+S +SAT
Sbjct: 99  RTISRFSAT 107
>XP_005252265
          Length = 1381

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>XP_005252264
          Length = 1478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>XP_006717348
          Length = 1541

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>XP_006717347
          Length = 1541

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>XP_006717346
          Length = 1541

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RTLSAYSA 8
            R LSAYSA
Sbjct: 1132 RLLSAYSA 1139
>XP_005265428
          Length = 1959

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTLSAYSAT 9
           RT+S +SAT
Sbjct: 99  RTISRFSAT 107
>NP_667338
          Length = 1308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLSAYSA 8
           TLSAY+A
Sbjct: 981 TLSAYTA 987
>NP_001159608
          Length = 1228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLSAYSA 8
           TLSAY+A
Sbjct: 901 TLSAYTA 907
>NP_001888
          Length = 2322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RTLSAYS 7
            RTLSA+S
Sbjct: 1415 RTLSAFS 1421
>NP_001268885
          Length = 3631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    TLSAYSAT 9
            TLS Y AT
Sbjct: 2670 TLSVYGAT 2677
>NP_443128
          Length = 3487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    TLSAYSAT 9
            TLS Y AT
Sbjct: 2672 TLSVYGAT 2679
>XP_006713923
          Length = 1228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLSAYSA 8
           TLSAY+A
Sbjct: 901 TLSAYTA 907
>XP_006713922
          Length = 1262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TLSAYSA 8
           TLSAY+A
Sbjct: 981 TLSAYTA 987
>XP_006712934
          Length = 1583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RTLSAY 6
            RTLSAY
Sbjct: 1189 RTLSAY 1194
>XP_006712933
          Length = 1687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RTLSAY 6
            RTLSAY
Sbjct: 1293 RTLSAY 1298
>NP_852118
          Length = 1684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RTLSAY 6
            RTLSAY
Sbjct: 1290 RTLSAY 1295
>NP_036252
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTLSAYSAT 9
           RTL AY  T
Sbjct: 275 RTLFAYEGT 283
>NP_443200
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LSAYSAT 9
          LSA+SAT
Sbjct: 15 LSAFSAT 21
>NP_001160070
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LSAYSAT 9
          LSA+SAT
Sbjct: 15 LSAFSAT 21
>NP_055698
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>NP_001015045
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  TLSAYSAT 9
          TLSA SAT
Sbjct: 70 TLSASSAT 77
>NP_001252509
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  TLSAYSAT 9
          TLSA SAT
Sbjct: 42 TLSASSAT 49
>NP_001252508
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  TLSAYSAT 9
          TLSA SAT
Sbjct: 70 TLSASSAT 77
>NP_001252507
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  TLSAYSAT 9
          TLSA SAT
Sbjct: 56 TLSASSAT 63
>NP_001001998
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RTLSAYSAT 9
          R LSA SAT
Sbjct: 10 RVLSATSAT 18
>XP_005262351
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LSAYSAT 9
           L AYSAT
Sbjct: 389 LQAYSAT 395
>XP_006718824
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LSAYSAT 9
          LSA+SAT
Sbjct: 15 LSAFSAT 21
>XP_005249034
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTLSAYSAT 9
           RTL AY  T
Sbjct: 275 RTLFAYEGT 283
>XP_005249033
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTLSAYSAT 9
           RTL AY  T
Sbjct: 271 RTLFAYEGT 279
>XP_005262742
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>XP_005262741
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 187 TLSASSAT 194
>XP_006714120
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 207 TLSASSAT 214
>XP_005262740
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 396 TLSASSAT 403
>XP_005262739
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 376 TLSASSAT 383
>XP_005262738
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TLSAYSAT 9
           TLSA SAT
Sbjct: 382 TLSASSAT 389
>XP_005263532
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RTLSAYSAT 9
          R LSA SAT
Sbjct: 34 RVLSATSAT 42
>NP_002676
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RTLSAYSAT 9
          R LSA SAT
Sbjct: 10 RVLSATSAT 18
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.106    0.258 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 355,013
Number of extensions: 339
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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