Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_001183955                                                           17    3301
NP_001183956                                                           17    3301
NP_116028                                                              17    3301
XP_005249954                                                           17    3301
NP_998821                                                              17    4312
NP_001229725                                                           17    4312
NP_001229724                                                           17    4312
NP_055484                                                              17    4312
XP_005256320                                                           17    4312
NP_055615                                                              16    5631
NP_115791                                                              16    5631
NP_001193411                                                           16    5631
NP_001193409                                                           16    5631
XP_005249976                                                           16    5631
XP_005249977                                                           16    5631
XP_006715868                                                           16    5631
XP_006715858                                                           16    5631
XP_006715857                                                           16    5631
NP_005742                                                              16    7355
NP_671714                                                              16    7355
NP_005952                                                              16    7355
XP_006725823                                                           16    7355
XP_006725595                                                           16    7355
XP_005250160                                                           16    7355
XP_005250159                                                           16    7355
NP_001128571                                                           15    9605
NP_115696                                                              15    9605
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_079082                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_001272829                                                           15    9605
NP_056347                                                              15    9605
XP_006724942                                                           15    9605
XP_006724111                                                           15    9605
XP_005261099                                                           15    9605
XP_005271738                                                           15    9605
XP_006718978                                                           15    9605
XP_005271736                                                           15    9605
XP_005271737                                                           15    9605
XP_005253222                                                           15    9605
XP_006718408                                                           15    9605
XP_005253221                                                           15    9605
XP_005246888                                                           15    9605
XP_006712788                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
XP_006710300                                                           15    9605
XP_006710331                                                           15    9605
NP_659411                                                              15    9605
NP_061964                                                              15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605
>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    RSSVDNTKM 9
            +SSVDNTKM
Sbjct: 1584 QSSVDNTKM 1592
>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    RSSVDNTKM 9
            +SSVDNTKM
Sbjct: 1584 QSSVDNTKM 1592
>NP_001183955
          Length = 519

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          SVDNT+M
Sbjct: 55 SVDNTRM 61
>NP_001183956
          Length = 459

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          SVDNT+M
Sbjct: 55 SVDNTRM 61
>NP_116028
          Length = 440

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          SVDNT+M
Sbjct: 55 SVDNTRM 61
>XP_005249954
          Length = 459

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          SVDNT+M
Sbjct: 55 SVDNTRM 61
>NP_998821
          Length = 491

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSVDNTKM 9
           S +DNTK+
Sbjct: 169 SQIDNTKL 176
>NP_001229725
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RSSVDNTKM 9
          RSS+DN +M
Sbjct: 52 RSSLDNIEM 60
>NP_001229724
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RSSVDNTKM 9
          RSS+DN +M
Sbjct: 52 RSSLDNIEM 60
>NP_055484
          Length = 819

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS+DN +M
Sbjct: 175 RSSLDNIEM 183
>XP_005256320
          Length = 819

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS+DN +M
Sbjct: 175 RSSLDNIEM 183
>NP_055615
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>NP_115791
          Length = 1154

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS+D TK+
Sbjct: 932 RSSLDATKL 940
>NP_001193411
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>NP_001193409
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>XP_005249976
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>XP_005249977
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>XP_006715868
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSSVDNTKM 9
           RSS++ TKM
Sbjct: 415 RSSIELTKM 423
>XP_006715858
          Length = 1267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RSSVDNTKM 9
            RSS+D TK+
Sbjct: 1045 RSSLDATKL 1053
>XP_006715857
          Length = 1268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RSSVDNTKM 9
            RSS+D TK+
Sbjct: 1046 RSSLDATKL 1054
>NP_005742
          Length = 3907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RSSVDNTKM 9
            RSS+DN  +
Sbjct: 1640 RSSIDNENL 1648
>NP_671714
          Length = 3899

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RSSVDNTKM 9
            RSS+DN  +
Sbjct: 1640 RSSIDNENL 1648
>NP_005952
          Length = 2439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RSSVDN 6
           RSSVDN
Sbjct: 654 RSSVDN 659
>XP_006725823
          Length = 1825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RSSVDN 6
           RSSVDN
Sbjct: 654 RSSVDN 659
>XP_006725595
          Length = 1311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RSSVDN 6
           RSSVDN
Sbjct: 654 RSSVDN 659
>XP_005250160
          Length = 3939

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RSSVDNTKM 9
            RSS+DN  +
Sbjct: 1640 RSSIDNENL 1648
>XP_005250159
          Length = 3952

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RSSVDNTKM 9
            RSS+DN  +
Sbjct: 1640 RSSIDNENL 1648
>NP_001128571
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          +VDN+KM
Sbjct: 32 AVDNSKM 38
>NP_115696
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSSVDNTKM 9
          +  VD+TKM
Sbjct: 41 KGGVDHTKM 49
>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RSSVDNTK 8
            R S+DN+K
Sbjct: 8256 RLSIDNSK 8263
>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RSSVDNTK 8
            R S+DN+K
Sbjct: 8381 RLSIDNSK 8388
>NP_079082
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSSVDNTKM 9
           RSS  NTK+
Sbjct: 639 RSSSKNTKL 647
>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1     RSSVDNTK 8
             R S+DN+K
Sbjct: 15680 RLSIDNSK 15687
>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1     RSSVDNTK 8
             R S+DN+K
Sbjct: 14753 RLSIDNSK 14760
>NP_001272829
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSVDNTK 8
           SSVDN K
Sbjct: 513 SSVDNIK 519
>NP_056347
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSVDNTK 8
           SSVDN K
Sbjct: 116 SSVDNIK 122
>XP_006724942
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RSSVDNT 7
           RSSVD T
Sbjct: 211 RSSVDTT 217
>XP_006724111
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          +VDN+KM
Sbjct: 32 AVDNSKM 38
>XP_005261099
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SVDNTKM 9
          +VDN+KM
Sbjct: 32 AVDNSKM 38
>XP_005271738
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSSVDNTKM 9
           RSS  NTK+
Sbjct: 639 RSSSKNTKL 647
>XP_006718978
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSSVDNTKM 9
           RSS  NTK+
Sbjct: 601 RSSSKNTKL 609
>XP_005271736
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSSVDNTKM 9
           RSS  NTK+
Sbjct: 639 RSSSKNTKL 647
>XP_005271737
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSSVDNTKM 9
           RSS  NTK+
Sbjct: 639 RSSSKNTKL 647
>XP_005253222
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSSVDNTKM 9
          +  VD+TKM
Sbjct: 49 KGGVDHTKM 57
>XP_006718408
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSSVDNTKM 9
          +  VD+TKM
Sbjct: 49 KGGVDHTKM 57
>XP_005253221
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSSVDNTKM 9
          +  VD+TKM
Sbjct: 49 KGGVDHTKM 57
>XP_005246888
          Length = 22663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RSSVDNTK 8
            R S+DN+K
Sbjct: 3993 RLSIDNSK 4000
>XP_006712788
          Length = 24022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RSSVDNTK 8
            R S+DN+K
Sbjct: 5352 RLSIDNSK 5359
>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1     RSSVDNTK 8
             R S+DN+K
Sbjct: 15308 RLSIDNSK 15315
>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1     RSSVDNTK 8
             R S+DN+K
Sbjct: 16814 RLSIDNSK 16821
>XP_006710300
          Length = 1116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSVDNTK 8
           SSVD TK
Sbjct: 982 SSVDTTK 988
>XP_006710331
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RSSVDNT 7
           RSSVD T
Sbjct: 211 RSSVDTT 217
>NP_659411
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  SSVDNTKM 9
          S+++NTK+
Sbjct: 81 SAIENTKL 88
>NP_061964
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SVDNTKM 9
          S DNT+M
Sbjct: 55 SADNTRM 61
>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1     RSSVDNTK 8
             R S+DN+K
Sbjct: 17321 RLSIDNSK 17328
>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RSSVDNTK 8
            R S+DN+K
Sbjct: 8448 RLSIDNSK 8455
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.108    0.261 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,830
Number of extensions: 455
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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