Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              19     869
NP_066124                                                              19     869
XP_006717999                                                           19     869
NP_001271250                                                           16    5631
NP_001271249                                                           16    5631
NP_149984                                                              16    5631
NP_001035191                                                           16    5631
NP_001271253                                                           16    5631
NP_001271251                                                           16    5631
NP_001271252                                                           16    5631
NP_001035192                                                           16    5631
NP_004802                                                              16    7355
NP_062832                                                              16    7355
NP_001137467                                                           16    7355
NP_001139186                                                           16    7355
XP_006723028                                                           16    7355
XP_006723027                                                           16    7355
XP_006723026                                                           16    7355
XP_006718813                                                           16    7355
NP_001139185                                                           16    7355
NP_005491                                                              16    7355
NP_003117                                                              15    9605
XP_006725621                                                           15    9605
XP_006725620                                                           15    9605
XP_006725617                                                           15    9605
XP_006725616                                                           15    9605
XP_006722903                                                           15    9605
XP_005259673                                                           15    9605
XP_005254677                                                           15    9605
XP_005254676                                                           15    9605
XP_005254674                                                           15    9605
XP_005254673                                                           15    9605
NP_001073992                                                           15    9605
>NP_065681
          Length = 1072

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RRASLDSME 9
           RR SLDSME
Sbjct: 693 RRPSLDSME 701
>NP_066124
          Length = 1114

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RRASLDSME 9
           RR SLDSME
Sbjct: 693 RRPSLDSME 701
>XP_006717999
          Length = 1207

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RRASLDSME 9
           RR SLDSME
Sbjct: 786 RRPSLDSME 794
>NP_001271250
          Length = 129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 44 RRSSIEAME 52
>NP_001271249
          Length = 130

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 45 RRSSIEAME 53
>NP_149984
          Length = 141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 56 RRSSIEAME 64
>NP_001035191
          Length = 118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 33 RRSSIEAME 41
>NP_001271253
          Length = 131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 46 RRSSIEAME 54
>NP_001271251
          Length = 131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 46 RRSSIEAME 54
>NP_001271252
          Length = 131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 46 RRSSIEAME 54
>NP_001035192
          Length = 131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RRASLDSME 9
          RR+S+++ME
Sbjct: 46 RRSSIEAME 54
>NP_004802
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RASLDSM 8
           +ASLDSM
Sbjct: 125 KASLDSM 131
>NP_062832
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           RR+SLD  E
Sbjct: 253 RRSSLDGSE 261
>NP_001137467
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RASLDSM 8
           +ASLDSM
Sbjct: 130 KASLDSM 136
>NP_001139186
          Length = 266

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 195 KRAKLDSSE 203
>XP_006723028
          Length = 281

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 231 KRAKLDSSE 239
>XP_006723027
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 131 KRAKLDSSE 139
>XP_006723026
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 231 KRAKLDSSE 239
>XP_006718813
          Length = 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RASLDSM 8
          +ASLDSM
Sbjct: 29 KASLDSM 35
>NP_001139185
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 195 KRAKLDSSE 203
>NP_005491
          Length = 346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           +RA LDS E
Sbjct: 195 KRAKLDSSE 203
>NP_003117
          Length = 2419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RASLDSME 9
            ++SLDS+E
Sbjct: 1423 KSSLDSLE 1430
>XP_006725621
          Length = 1735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_006725620
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_006725617
          Length = 1815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_006725616
          Length = 1835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_006722903
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           RR+ LDS E
Sbjct: 103 RRSRLDSSE 111
>XP_005259673
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           RR+ LDS E
Sbjct: 103 RRSRLDSSE 111
>XP_005254677
          Length = 1735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_005254676
          Length = 1755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_005254674
          Length = 1815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>XP_005254673
          Length = 1835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RRASLDSME 9
          R  SLDSM+
Sbjct: 63 RSKSLDSMD 71
>NP_001073992
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRASLDSME 9
           RR+ LDS E
Sbjct: 241 RRSRLDSSE 249
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.118    0.281 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 368,117
Number of extensions: 572
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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