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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     509
XP_005266703                                                           20     509
NP_620450                                                              18    1482
NP_001094832                                                           17    4312
NP_001171953                                                           17    4312
XP_006724302                                                           17    4312
XP_006720432                                                           17    4312
NP_037505                                                              17    4312
NP_055244                                                              16    5631
NP_001129687                                                           16    5631
NP_476432                                                              16    5631
XP_005247419                                                           16    5631
XP_006711087                                                           16    5631
XP_006711086                                                           16    5631
NP_476431                                                              16    5631
NP_002817                                                              16    7355
NP_001004128                                                           16    7355
NP_000426                                                              16    7355
NP_060252                                                              16    7355
XP_005259981                                                           16    7355
XP_005252540                                                           16    7355
XP_005252539                                                           16    7355
XP_005252538                                                           16    7355
XP_005252537                                                           16    7355
XP_005252535                                                           16    7355
XP_005252534                                                           16    7355
XP_006717515                                                           16    7355
XP_005252533                                                           16    7355
XP_005252532                                                           16    7355
XP_005252531                                                           16    7355
NP_001013373                                                           16    7355
NP_001155155                                                           15    9605
NP_733764                                                              15    9605
NP_653273                                                              15    9605
NP_001514                                                              15    9605
XP_005258891                                                           15    9605

>NP_443179
          Length = 2170

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    PAKYLAVS 9
            PAKYLAVS
Sbjct: 1019 PAKYLAVS 1026



>XP_005266703
          Length = 1501

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKYLAVS
Sbjct: 350 PAKYLAVS 357



>NP_620450
          Length = 271

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RPAKYLAVS 9
           RPAKYL+++
Sbjct: 215 RPAKYLSLA 223



>NP_001094832
          Length = 458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP+ Y AVS
Sbjct: 153 RPSSYAAVS 161



>NP_001171953
          Length = 778

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RPAKYLAVS 9
          +PAKY+ V+
Sbjct: 10 KPAKYIPVA 18



>XP_006724302
          Length = 778

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RPAKYLAVS 9
          +PAKY+ V+
Sbjct: 10 KPAKYIPVA 18



>XP_006720432
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  PAKYLAVS 9
          PA++LAVS
Sbjct: 86 PAQFLAVS 93



>NP_037505
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RPAKYLAVS 9
          +PAKY+ V+
Sbjct: 10 KPAKYIPVA 18



>NP_055244
          Length = 984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP KYL V+
Sbjct: 270 RPRKYLYVT 278



>NP_001129687
          Length = 228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP +Y AVS
Sbjct: 197 RPTRYEAVS 205



>NP_476432
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP +Y AVS
Sbjct: 189 RPTRYEAVS 197



>XP_005247419
          Length = 1014

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP KYL V+
Sbjct: 270 RPRKYLYVT 278



>XP_006711087
          Length = 237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP +Y AVS
Sbjct: 206 RPTRYEAVS 214



>XP_006711086
          Length = 237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP +Y AVS
Sbjct: 206 RPTRYEAVS 214



>NP_476431
          Length = 228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RPAKYLAVS 9
           RP +Y AVS
Sbjct: 197 RPTRYEAVS 205



>NP_002817
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  RPAKYLA 7
          RPA YLA
Sbjct: 91 RPALYLA 97



>NP_001004128
          Length = 604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  RPAKYLA 7
          RPA YLA
Sbjct: 91 RPALYLA 97



>NP_000426
          Length = 2321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    RPAKYLAV 8
            RP  YLAV
Sbjct: 2207 RPPPYLAV 2214



>NP_060252
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005259981
          Length = 2269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    RPAKYLAV 8
            RP  YLAV
Sbjct: 2155 RPPPYLAV 2162



>XP_005252540
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252539
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252538
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252537
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252535
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252534
          Length = 2521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 911 PAKYVSIN 918



>XP_006717515
          Length = 2521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 911 PAKYVSIN 918



>XP_005252533
          Length = 2521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 911 PAKYVSIN 918



>XP_005252532
          Length = 2430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   PAKYLAVS 9
           PAKY++++
Sbjct: 820 PAKYVSIN 827



>XP_005252531
          Length = 2670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    PAKYLAVS 9
            PAKY++++
Sbjct: 1060 PAKYVSIN 1067



>NP_001013373
          Length = 316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RPAKYLAV 8
           RP +Y+A+
Sbjct: 128 RPLRYIAI 135



>NP_001155155
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PAKYLA 7
           PA+YLA
Sbjct: 548 PARYLA 553



>NP_733764
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PAKYLA 7
           PA+YLA
Sbjct: 303 PARYLA 308



>NP_653273
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PAKYLA 7
           PA+YLA
Sbjct: 627 PARYLA 632



>NP_001514
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PAKYLAV 8
           PAK+LA+
Sbjct: 474 PAKFLAL 480



>XP_005258891
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PAKYLAV 8
           PAK+LA+
Sbjct: 473 PAKFLAL 479



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.128    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 644,581
Number of extensions: 1066
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)