Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_071748                                                              17    4312
XP_006712749                                                           17    4312
XP_006712748                                                           17    4312
XP_005246823                                                           17    4312
XP_005246822                                                           17    4312
XP_005246821                                                           17    4312
XP_005246820                                                           17    4312
NP_056263                                                              16    5631
XP_006717808                                                           16    5631
NP_001139669                                                           16    5631
NP_219500                                                              16    5631
NP_056327                                                              16    7355
NP_001269701                                                           16    7355
NP_061887                                                              16    7355
NP_001269706                                                           16    7355
NP_001269708                                                           16    7355
NP_001269705                                                           16    7355
NP_001131076                                                           16    7355
NP_001010924                                                           16    7355
XP_006713163                                                           16    7355
NP_001005407                                                           15    9605
NP_066921                                                              15    9605
NP_733751                                                              15    9605
NP_005898                                                              15    9605
NP_056045                                                              15    9605
NP_542192                                                              15    9605
XP_006723769                                                           15    9605
XP_006721032                                                           15    9605
XP_006721031                                                           15    9605
XP_006721030                                                           15    9605
XP_006721029                                                           15    9605
XP_006721028                                                           15    9605
XP_006721027                                                           15    9605
XP_005255709                                                           15    9605
XP_006721026                                                           15    9605
XP_006716142                                                           15    9605
XP_005250088                                                           15    9605
XP_006716141                                                           15    9605
XP_005250085                                                           15    9605
XP_006716140                                                           15    9605
XP_005250084                                                           15    9605
XP_005250083                                                           15    9605
XP_005250082                                                           15    9605
XP_005267043                                                           15    9605
XP_005262699                                                           15    9605
XP_005262697                                                           15    9605
XP_006714054                                                           15    9605
XP_005262695                                                           15    9605
>NP_071748
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 370 RLRAGLGIL 378
>XP_006712749
          Length = 267

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 223 RLRAGLGIL 231
>XP_006712748
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 370 RLRAGLGIL 378
>XP_005246823
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 370 RLRAGLGIL 378
>XP_005246822
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 381 RLRAGLGIL 389
>XP_005246821
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 381 RLRAGLGIL 389
>XP_005246820
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RMKSGLGVM 9
           R+++GLG++
Sbjct: 381 RLRAGLGIL 389
>NP_056263
          Length = 203

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RMKSGLGVM 9
          R+KS LG M
Sbjct: 53 RVKSALGAM 61
>XP_006717808
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+KS LG M
Sbjct: 223 RVKSALGAM 231
>NP_001139669
          Length = 7081

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RMKSGLGVM 9
            ++KSG GVM
Sbjct: 5192 KVKSGPGVM 5200
>NP_219500
          Length = 112

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RMKSGLGVM 9
          R+ SGLG+M
Sbjct: 51 RVLSGLGLM 59
>NP_056327
          Length = 4265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RMKSGL 6
            RMKSGL
Sbjct: 2831 RMKSGL 2836
>NP_001269701
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 478 RSKTGLGLL 486
>NP_061887
          Length = 1135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 440 RSKTGLGLL 448
>NP_001269706
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 301 RSKTGLGLL 309
>NP_001269708
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 301 RSKTGLGLL 309
>NP_001269705
          Length = 924

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 229 RSKTGLGLL 237
>NP_001131076
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           R K+GLG++
Sbjct: 384 RSKTGLGLL 392
>NP_001010924
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MKSGLGVM 9
           +KSGLG++
Sbjct: 255 LKSGLGLV 262
>XP_006713163
          Length = 4265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RMKSGL 6
            RMKSGL
Sbjct: 2831 RMKSGL 2836
>NP_001005407
          Length = 2347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>NP_066921
          Length = 2353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>NP_733751
          Length = 4911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 911 KLKSGIGAV 919
>NP_005898
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKSGLG 7
           MKSG+G
Sbjct: 329 MKSGIG 334
>NP_056045
          Length = 1704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KSGLGV 8
           KSGLG+
Sbjct: 622 KSGLGI 627
>NP_542192
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MKSGLGV 8
           +K+GLGV
Sbjct: 153 LKTGLGV 159
>XP_006723769
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MKSGLGV 8
           +K+GLGV
Sbjct: 153 LKTGLGV 159
>XP_006721032
          Length = 1566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721031
          Length = 1612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721030
          Length = 1618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721029
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721028
          Length = 2336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721027
          Length = 2342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_005255709
          Length = 2347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006721026
          Length = 2358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RMKSGLGVM 9
           R+ +GLG M
Sbjct: 603 RLATGLGTM 611
>XP_006716142
          Length = 4927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_005250088
          Length = 4928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_006716141
          Length = 4959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_005250085
          Length = 4979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_006716140
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_005250084
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_005250083
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 911 KLKSGIGAV 919
>XP_005250082
          Length = 4983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RMKSGLGVM 9
           ++KSG+G +
Sbjct: 912 KLKSGIGAV 920
>XP_005267043
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   MKSGLG 7
           MKSG+G
Sbjct: 412 MKSGIG 417
>XP_005262699
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KSGLGV 8
           KSGLG+
Sbjct: 622 KSGLGI 627
>XP_005262697
          Length = 1681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KSGLGV 8
           KSGLG+
Sbjct: 622 KSGLGI 627
>XP_006714054
          Length = 1693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KSGLGV 8
           KSGLG+
Sbjct: 611 KSGLGI 616
>XP_005262695
          Length = 1704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KSGLGV 8
           KSGLG+
Sbjct: 622 KSGLGI 627
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.139    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 546,480
Number of extensions: 813
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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