Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           18    1935
NP_001245200                                                           18    1935
NP_001161091                                                           18    1935
NP_000240                                                              18    1935
NP_001161090                                                           18    1935
NP_001161089                                                           18    1935
NP_001245203                                                           18    1935
NP_001243000                                                           18    1935
XP_005257603                                                           18    1935
XP_006722058                                                           18    1935
XP_005257602                                                           18    1935
XP_005265221                                                           18    1935
XP_005265220                                                           18    1935
NP_001010987                                                           16    5631
NP_075055                                                              15    9605
XP_006722904                                                           15    9605
XP_005260077                                                           15    9605

>NP_001245202
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>NP_001245200
          Length = 687

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RLVESISLR 9
           RLVES SLR
Sbjct: 265 RLVESTSLR 273



>NP_001161091
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>NP_000240
          Length = 756

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RLVESISLR 9
           RLVES SLR
Sbjct: 265 RLVESTSLR 273



>NP_001161090
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>NP_001161089
          Length = 658

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RLVESISLR 9
           RLVES SLR
Sbjct: 167 RLVESTSLR 175



>NP_001245203
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>NP_001243000
          Length = 5207

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RLVESISL 8
            RLVESISL
Sbjct: 2600 RLVESISL 2607



>XP_005257603
          Length = 5256

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RLVESISL 8
            RLVESISL
Sbjct: 2649 RLVESISL 2656



>XP_006722058
          Length = 5256

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RLVESISL 8
            RLVESISL
Sbjct: 2649 RLVESISL 2656



>XP_005257602
          Length = 5256

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RLVESISL 8
            RLVESISL
Sbjct: 2649 RLVESISL 2656



>XP_005265221
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>XP_005265220
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLVESISLR 9
          RLVES SLR
Sbjct: 24 RLVESTSLR 32



>NP_001010987
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RLVESISL 8
           R+VES+SL
Sbjct: 435 RVVESVSL 442



>NP_075055
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLVESIS 7
           RLVES+S
Sbjct: 910 RLVESLS 916



>XP_006722904
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLVESIS 7
           RLVES+S
Sbjct: 445 RLVESLS 451



>XP_005260077
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLVESIS 7
           RLVES+S
Sbjct: 904 RLVESLS 910



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.139    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 234,827
Number of extensions: 161
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)