Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057502                                                              20     390
NP_861969                                                              20     390
NP_000259                                                              20     390
NP_861968                                                              20     390
NP_861966                                                              20     390
NP_861970                                                              20     390
NP_861546                                                              20     390
NP_861967                                                              20     390
NP_002435                                                              18    1135
XP_005262326                                                           18    1135
NP_003370                                                              17    2528
NP_001104547                                                           17    2528
NP_001247421                                                           17    2528
NP_001247423                                                           17    2528
NP_001247422                                                           17    2528
NP_002897                                                              17    2528
XP_006715624                                                           17    2528
NP_006141                                                              17    3301
XP_005272662                                                           17    3301
NP_005137                                                              17    3301
NP_001269569                                                           16    5631
NP_001269572                                                           16    5631
NP_071931                                                              16    5631
NP_065801                                                              16    5631
NP_001188405                                                           16    5631
NP_113634                                                              16    5631
XP_005258421                                                           16    5631
NP_001231509                                                           16    7355
NP_003487                                                              16    7355
NP_065811                                                              16    7355
XP_006712712                                                           16    7355
XP_006712711                                                           16    7355
XP_006712710                                                           16    7355
XP_006712709                                                           16    7355
NP_001277007                                                           15    9605
NP_000450                                                              15    9605
NP_001277006                                                           15    9605
NP_001020414                                                           15    9605
NP_001020413                                                           15    9605
NP_001560                                                              15    9605
NP_057191                                                              15    9605
NP_056473                                                              15    9605
NP_003747                                                              15    9605
XP_005274725                                                           15    9605
XP_005255669                                                           15    9605
XP_005251620                                                           15    9605
XP_005251618                                                           15    9605
XP_005250685                                                           15    9605
XP_005250684                                                           15    9605
XP_005250683                                                           15    9605
XP_005250682                                                           15    9605
XP_006714540                                                           15    9605
XP_006713468                                                           15    9605
XP_005265616                                                           15    9605
>NP_057502
          Length = 590

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 249 RLTPKISF 256
>NP_861969
          Length = 507

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 166 RLTPKISF 173
>NP_000259
          Length = 595

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 249 RLTPKISF 256
>NP_861968
          Length = 507

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 166 RLTPKISF 173
>NP_861966
          Length = 548

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 207 RLTPKISF 214
>NP_861970
          Length = 590

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 249 RLTPKISF 256
>NP_861546
          Length = 590

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 249 RLTPKISF 256
>NP_861967
          Length = 549

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RLTPKISF 8
           RLTPKISF
Sbjct: 208 RLTPKISF 215
>NP_002435
          Length = 577

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           RLTPKI F
Sbjct: 233 RLTPKIGF 240
>XP_005262326
          Length = 578

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           RLTPKI F
Sbjct: 234 RLTPKIGF 241
>NP_003370
          Length = 586

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>NP_001104547
          Length = 586

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>NP_001247421
          Length = 604

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>NP_001247423
          Length = 447

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 97  KLTPKIGF 104
>NP_001247422
          Length = 604

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>NP_002897
          Length = 583

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>XP_006715624
          Length = 543

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           +LTPKI F
Sbjct: 233 KLTPKIGF 240
>NP_006141
          Length = 615

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TPKISFR 9
           TP++SFR
Sbjct: 471 TPRVSFR 477
>XP_005272662
          Length = 547

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TPKISFR 9
           TP++SFR
Sbjct: 403 TPRVSFR 409
>NP_005137
          Length = 800

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RLTPKISFR 9
           +LTPK +FR
Sbjct: 719 KLTPKEAFR 727
>NP_001269569
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           R+ PKIS+
Sbjct: 320 RINPKISY 327
>NP_001269572
          Length = 722

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           R+ PKIS+
Sbjct: 320 RINPKISY 327
>NP_071931
          Length = 747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLTPKISF 8
           R+ PKIS+
Sbjct: 320 RINPKISY 327
>NP_065801
          Length = 1204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RLTPKISFR 9
           RL PK SF+
Sbjct: 452 RLDPKTSFQ 460
>NP_001188405
          Length = 130

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RLTPKI 6
          RLTPKI
Sbjct: 64 RLTPKI 69
>NP_113634
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RLTPKI 6
          RLTPKI
Sbjct: 64 RLTPKI 69
>XP_005258421
          Length = 226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RLTPKI 6
          RLTPKI
Sbjct: 70 RLTPKI 75
>NP_001231509
          Length = 3859

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLTPKIS 7
            RLTP IS
Sbjct: 3730 RLTPNIS 3736
>NP_003487
          Length = 3830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLTPKIS 7
            RLTP IS
Sbjct: 3701 RLTPNIS 3707
>NP_065811
          Length = 1572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TPKISFR 9
           TPK SFR
Sbjct: 381 TPKHSFR 387
>XP_006712712
          Length = 1414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TPKISFR 9
           TPK SFR
Sbjct: 223 TPKHSFR 229
>XP_006712711
          Length = 1448

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TPKISFR 9
           TPK SFR
Sbjct: 257 TPKHSFR 263
>XP_006712710
          Length = 1528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TPKISFR 9
           TPK SFR
Sbjct: 388 TPKHSFR 394
>XP_006712709
          Length = 1579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TPKISFR 9
           TPK SFR
Sbjct: 388 TPKHSFR 394
>NP_001277007
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           R+TPKI
Sbjct: 200 RMTPKI 205
>NP_000450
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           R+TPKI
Sbjct: 347 RMTPKI 352
>NP_001277006
          Length = 1081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           R+TPKI
Sbjct: 304 RMTPKI 309
>NP_001020414
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 351 RLTPKL 356
>NP_001020413
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 351 RLTPKL 356
>NP_001560
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 351 RLTPKL 356
>NP_057191
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TPKISF 8
           TPKI+F
Sbjct: 499 TPKINF 504
>NP_056473
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 164 RLTPKL 169
>NP_003747
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 168 RLTPKL 173
>XP_005274725
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           RLTPK+
Sbjct: 377 RLTPKL 382
>XP_005255669
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  RLTPKISF 8
          RL P ISF
Sbjct: 20 RLAPVISF 27
>XP_005251620
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           R+TPKI
Sbjct: 304 RMTPKI 309
>XP_005251618
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RLTPKI 6
           R+TPKI
Sbjct: 347 RMTPKI 352
>XP_005250685
          Length = 193

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RLTPKISFR 9
           ++ P+I+FR
Sbjct: 174 KIKPRITFR 182
>XP_005250684
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RLTPKISFR 9
           ++ P+I+FR
Sbjct: 185 KIKPRITFR 193
>XP_005250683
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RLTPKISFR 9
           ++ P+I+FR
Sbjct: 185 KIKPRITFR 193
>XP_005250682
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RLTPKISFR 9
           ++ P+I+FR
Sbjct: 185 KIKPRITFR 193
>XP_006714540
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TPKISF 8
           TPKI+F
Sbjct: 455 TPKINF 460
>XP_006713468
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 LTPKISF 8
         +TPKIS+
Sbjct: 1 MTPKISW 7
>XP_005265616
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LTPKISF 8
          +TPKIS+
Sbjct: 56 MTPKISW 62
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.145    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 685,468
Number of extensions: 804
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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