Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     665
NP_000170                                                              19     665
NP_001268422                                                           19     665
NP_001268423                                                           19     665
XP_005264328                                                           19     665
NP_061992                                                              17    4312
NP_004269                                                              17    4312
NP_061761                                                              17    4312
XP_006721659                                                           17    4312
NP_061993                                                              17    4312
NP_065782                                                              16    5631
NP_001229341                                                           16    5631
NP_055490                                                              16    7355
XP_006726469                                                           16    7355
XP_006713516                                                           16    7355
XP_006711123                                                           16    7355
XP_006711122                                                           16    7355
XP_006711121                                                           16    7355
XP_006711120                                                           16    7355
XP_006711119                                                           16    7355
NP_001158268                                                           15    9605
NP_066008                                                              15    9605
NP_064506                                                              15    9605
NP_775742                                                              15    9605
NP_005877                                                              15    9605
NP_005489                                                              15    9605
NP_660289                                                              15    9605
NP_061757                                                              15    9605
NP_061759                                                              15    9605
NP_061760                                                              15    9605
NP_061758                                                              15    9605
NP_056484                                                              15    9605
NP_061763                                                              15    9605
XP_005259758                                                           15    9605
XP_006721186                                                           15    9605
XP_005255829                                                           15    9605
XP_006721185                                                           15    9605
XP_006721184                                                           15    9605
XP_006718117                                                           15    9605
XP_005269573                                                           15    9605
XP_005269572                                                           15    9605
XP_006713019                                                           15    9605
NP_061762                                                              15    9605
NP_061753                                                              15    9605
NP_061754                                                              15    9605
>NP_001268421
          Length = 1230

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLGASDRTM 9
            RLGASDR M
Sbjct: 1046 RLGASDRIM 1054
>NP_000170
          Length = 1360

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLGASDRTM 9
            RLGASDR M
Sbjct: 1176 RLGASDRIM 1184
>NP_001268422
          Length = 1058

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RLGASDRTM 9
           RLGASDR M
Sbjct: 874 RLGASDRIM 882
>NP_001268423
          Length = 1058

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RLGASDRTM 9
           RLGASDR M
Sbjct: 874 RLGASDRIM 882
>XP_005264328
          Length = 1261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLGASDRTM 9
            RLGASDR M
Sbjct: 1077 RLGASDRIM 1085
>NP_061992
          Length = 797

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GASDR
Sbjct: 530 RVGASDR 536
>NP_004269
          Length = 252

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RLGASDRTM 9
          RLGA  RT+
Sbjct: 36 RLGAESRTL 44
>NP_061761
          Length = 795

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GASDR
Sbjct: 529 RVGASDR 535
>XP_006721659
          Length = 244

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RLGASDRTM 9
          RLGA  RT+
Sbjct: 36 RLGAESRTL 44
>NP_061993
          Length = 801

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GASDR
Sbjct: 531 RVGASDR 537
>NP_065782
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LGASDRT 8
           LGASDR+
Sbjct: 577 LGASDRS 583
>NP_001229341
          Length = 701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LGASDRT 8
           LGASDR+
Sbjct: 559 LGASDRS 565
>NP_055490
          Length = 2017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1669 RLGLSDR 1675
>XP_006726469
          Length = 175

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LGASDRTM 9
           +GA DRT+
Sbjct: 161 VGAEDRTL 168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  RLGASDR 7
          R GASDR
Sbjct: 49 RRGASDR 55
>XP_006713516
          Length = 263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LGASDRTM 9
           +GA DRT+
Sbjct: 249 VGAEDRTL 256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RLGASDR 7
           R GASDR
Sbjct: 137 RRGASDR 143
>XP_006711123
          Length = 1737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1631 RLGLSDR 1637
>XP_006711122
          Length = 1918

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1631 RLGLSDR 1637
>XP_006711121
          Length = 1945

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1597 RLGLSDR 1603
>XP_006711120
          Length = 1972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1631 RLGLSDR 1637
>XP_006711119
          Length = 1979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            RLG SDR
Sbjct: 1631 RLGLSDR 1637
>NP_001158268
          Length = 186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LGASDRT 8
           LGA DRT
Sbjct: 168 LGAYDRT 174
>NP_066008
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>NP_064506
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    RLGASDR 7
            R+GA DR
Sbjct: 1402 RIGAGDR 1408
>NP_775742
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LGASDRT 8
           LGA DRT
Sbjct: 256 LGAYDRT 262
>NP_005877
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GASDRTM 9
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>NP_005489
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RLGASDRTM 9
           RLG +DR +
Sbjct: 211 RLGLNDRVL 219
>NP_660289
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLGASDRT 8
           RLG +D+T
Sbjct: 97  RLGENDKT 104
>NP_061757
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>NP_061759
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 532 RVGAADR 538
>NP_061760
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>NP_061758
          Length = 787

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>NP_056484
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 529 RVGATDR 535
>NP_061763
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>XP_005259758
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RLGASDRTM 9
           RLG +DR +
Sbjct: 211 RLGLNDRVL 219
>XP_006721186
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GASDRTM 9
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_005255829
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GASDRTM 9
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721185
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GASDRTM 9
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721184
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GASDRTM 9
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006718117
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RLGASDRT 8
          RLG ++RT
Sbjct: 69 RLGVAERT 76
>XP_005269573
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLGASDRT 8
           RLG +D+T
Sbjct: 97  RLGENDKT 104
>XP_005269572
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RLGASDRT 8
           RLG +D+T
Sbjct: 97  RLGENDKT 104
>XP_006713019
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LGASDRT 8
           LGA DRT
Sbjct: 241 LGAYDRT 247
>NP_061762
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 528 RVGATDR 534
>NP_061753
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
>NP_061754
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLGASDR 7
           R+GA+DR
Sbjct: 530 RVGATDR 536
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.129    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 447,829
Number of extensions: 553
Number of successful extensions: 47
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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