Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000412                                                              17    3301
XP_005257400                                                           17    3301
NP_940852                                                              17    4312
XP_005262137                                                           17    4312
NP_055490                                                              16    7355
XP_006712122                                                           16    7355
XP_006712121                                                           16    7355
XP_006711122                                                           16    7355
XP_006711121                                                           16    7355
XP_006711120                                                           16    7355
XP_006711119                                                           16    7355
NP_758454                                                              15    9605
NP_002964                                                              15    9605
NP_001035089                                                           15    9605
NP_001171869                                                           15    9605
NP_671735                                                              15    9605
NP_001171872                                                           15    9605
NP_001171874                                                           15    9605
NP_001171870                                                           15    9605
NP_001193937                                                           15    9605
NP_001171871                                                           15    9605
NP_060564                                                              15    9605
NP_001123479                                                           15    9605
NP_001123478                                                           15    9605
NP_008999                                                              15    9605
NP_001265137                                                           15    9605
NP_002085                                                              15    9605
NP_997263                                                              15    9605
XP_006724176                                                           15    9605
XP_006724175                                                           15    9605
XP_006724174                                                           15    9605
XP_006724173                                                           15    9605
XP_005255332                                                           15    9605
XP_005255331                                                           15    9605
XP_006716803                                                           15    9605
XP_005251459                                                           15    9605
XP_006715557                                                           15    9605
XP_006715555                                                           15    9605
XP_006715554                                                           15    9605
XP_006715553                                                           15    9605
XP_006715552                                                           15    9605
XP_005267054                                                           15    9605
XP_005267053                                                           15    9605
XP_006715551                                                           15    9605
XP_006715550                                                           15    9605
XP_006715549                                                           15    9605
XP_006715548                                                           15    9605
XP_006715547                                                           15    9605
XP_006715546                                                           15    9605
XP_006714181                                                           15    9605
NP_003773                                                              15    9605
>NP_000412
          Length = 584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RISARKGSL 9
          RIS+ KGSL
Sbjct: 35 RISSSKGSL 43
>XP_005257400
          Length = 624

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RISARKGSL 9
          RIS+ KGSL
Sbjct: 35 RISSSKGSL 43
>NP_940852
          Length = 268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISARKGS 8
           +SARKGS
Sbjct: 127 VSARKGS 133
>XP_005262137
          Length = 271

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISARKGS 8
           +SARKGS
Sbjct: 127 VSARKGS 133
>NP_055490
          Length = 2017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RISARKGSL 9
            R+SA KG L
Sbjct: 1898 RLSAEKGRL 1906
>XP_006712122
          Length = 801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RISARKG 7
           R+SAR+G
Sbjct: 790 RVSARRG 796
>XP_006712121
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RISARKG 7
           R+SAR+G
Sbjct: 790 RVSARRG 796
>XP_006711122
          Length = 1918

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RISARKGSL 9
            R+SA KG L
Sbjct: 1860 RLSAEKGRL 1868
>XP_006711121
          Length = 1945

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RISARKGSL 9
            R+SA KG L
Sbjct: 1826 RLSAEKGRL 1834
>XP_006711120
          Length = 1972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RISARKGSL 9
            R+SA KG L
Sbjct: 1860 RLSAEKGRL 1868
>XP_006711119
          Length = 1979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RISARKGSL 9
            R+SA KG L
Sbjct: 1860 RLSAEKGRL 1868
>NP_758454
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RISAR G
Sbjct: 326 RISARSG 332
>NP_002964
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RISARKGSL 9
           R+SA +GS+
Sbjct: 635 RVSAGRGSI 643
>NP_001035089
          Length = 1651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1112 RISDRRGS 1119
>NP_001171869
          Length = 185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 60 ARKGSL 65
>NP_671735
          Length = 168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 43 ARKGSL 48
>NP_001171872
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 43 ARKGSL 48
>NP_001171874
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 32 ARKGSL 37
>NP_001171870
          Length = 184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 60 ARKGSL 65
>NP_001193937
          Length = 1743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1095 RISDRRGS 1102
>NP_001171871
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 49 ARKGSL 54
>NP_060564
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 312 ISARKG 317
>NP_001123479
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 183 ISARKG 188
>NP_001123478
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 320 ISARKG 325
>NP_008999
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 300 RISLRKG 306
>NP_001265137
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 245 RISLRKG 251
>NP_002085
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 321 ISARKG 326
>NP_997263
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SARKGS 8
           SARKGS
Sbjct: 212 SARKGS 217
>XP_006724176
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 231 RISLRKG 237
>XP_006724175
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 245 RISLRKG 251
>XP_006724174
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 286 RISLRKG 292
>XP_006724173
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RIS RKG
Sbjct: 300 RISLRKG 306
>XP_005255332
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 213 ISARKG 218
>XP_005255331
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ISARKG 7
           ISARKG
Sbjct: 214 ISARKG 219
>XP_006716803
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 19 ARKGSL 24
>XP_005251459
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ARKGSL 9
          ARKGSL
Sbjct: 19 ARKGSL 24
>XP_006715557
          Length = 1744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1096 RISDRRGS 1103
>XP_006715555
          Length = 1638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1120 RISDRRGS 1127
>XP_006715554
          Length = 1768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1120 RISDRRGS 1127
>XP_006715553
          Length = 1808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1096 RISDRRGS 1103
>XP_006715552
          Length = 1817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1105 RISDRRGS 1112
>XP_005267054
          Length = 1823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1113 RISDRRGS 1120
>XP_005267053
          Length = 1824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1112 RISDRRGS 1119
>XP_006715551
          Length = 1825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1113 RISDRRGS 1120
>XP_006715550
          Length = 1830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1120 RISDRRGS 1127
>XP_006715549
          Length = 1830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1118 RISDRRGS 1125
>XP_006715548
          Length = 1831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1119 RISDRRGS 1126
>XP_006715547
          Length = 1831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1119 RISDRRGS 1126
>XP_006715546
          Length = 1832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RISARKGS 8
            RIS R+GS
Sbjct: 1120 RISDRRGS 1127
>XP_006714181
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RISARKG 7
           RISAR G
Sbjct: 326 RISARSG 332
>NP_003773
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISARKGSL 9
           +SAR+G L
Sbjct: 296 VSARRGGL 303
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.130    0.322 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 455,544
Number of extensions: 704
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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