Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              19     665
XP_006715292                                                           19     665
NP_775771                                                              19     869
XP_006720466                                                           19     869
XP_005254230                                                           19     869
XP_006720465                                                           19     869
XP_005254228                                                           19     869
NP_660204                                                              17    3301
NP_002370                                                              16    5631
NP_060453                                                              16    5631
NP_660295                                                              16    5631
XP_005257554                                                           16    5631
NP_085048                                                              16    7355
NP_005449                                                              16    7355
XP_005252373                                                           16    7355
NP_001885                                                              15    9605
NP_689407                                                              15    9605
NP_001239268                                                           15    9605
NP_001239267                                                           15    9605
NP_001785                                                              15    9605
NP_005522                                                              15    9605
NP_001193496                                                           15    9605
NP_001276841                                                           15    9605
NP_001020276                                                           15    9605
NP_055455                                                              15    9605
NP_001258671                                                           15    9605
NP_001258670                                                           15    9605
NP_001883                                                              15    9605
NP_001884                                                              15    9605
NP_620693                                                              15    9605
XP_005256394                                                           15    9605
XP_005256393                                                           15    9605
XP_005247035                                                           15    9605
XP_006712939                                                           15    9605
XP_006712938                                                           15    9605
XP_006712937                                                           15    9605
XP_006712936                                                           15    9605
XP_005247034                                                           15    9605
XP_006711353                                                           15    9605
XP_005245184                                                           15    9605
>NP_115927
          Length = 1321

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYASV
Sbjct: 200 RGTVRYASV 208
>XP_006715292
          Length = 759

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYASV
Sbjct: 187 RGTVRYASV 195
>NP_775771
          Length = 1244

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYAS+
Sbjct: 187 RGTVRYASI 195
>XP_006720466
          Length = 1175

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYAS+
Sbjct: 118 RGTVRYASI 126
>XP_005254230
          Length = 1175

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYAS+
Sbjct: 118 RGTVRYASI 126
>XP_006720465
          Length = 1239

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYAS+
Sbjct: 182 RGTVRYASI 190
>XP_005254228
          Length = 1250

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           RG VRYAS+
Sbjct: 193 RGTVRYASI 201
>NP_660204
          Length = 337

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GMVRYASV 9
           G VRYAS+
Sbjct: 183 GTVRYASI 190
>NP_002370
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GMVRYAS 8
          GMV YAS
Sbjct: 81 GMVNYAS 87
>NP_060453
          Length = 446

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RGMVRYASV 9
           RGM RY ++
Sbjct: 227 RGMKRYVTI 235
>NP_660295
          Length = 208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RGMVRYAS 8
           RGMV Y S
Sbjct: 131 RGMVMYTS 138
>XP_005257554
          Length = 364

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RGMVRYASV 9
           RGM RY ++
Sbjct: 145 RGMKRYVTI 153
>NP_085048
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   RGMVRYASV 9
           RG + YA V
Sbjct: 194 RGFINYAKV 202
>NP_005449
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GMVRYASV 9
           GM+ YAS+
Sbjct: 527 GMLSYASI 534
>XP_005252373
          Length = 683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GMVRYASV 9
           GM+ YAS+
Sbjct: 269 GMLSYASI 276
>NP_001885
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>NP_689407
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>NP_001239268
          Length = 842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           GMVRY  V
Sbjct: 238 GMVRYRIV 245
>NP_001239267
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           GMVRY  V
Sbjct: 275 GMVRYRIV 282
>NP_001785
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           GMVRY  V
Sbjct: 312 GMVRYRIV 319
>NP_005522
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           +G++R ASV
Sbjct: 607 KGLIRSASV 615
>NP_001193496
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           +G++R ASV
Sbjct: 607 KGLIRSASV 615
>NP_001276841
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>NP_001020276
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 211 GTARYASI 218
>NP_055455
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>NP_001258671
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 122 GTARYASI 129
>NP_001258670
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 183 GTARYASI 190
>NP_001883
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 183 GTARYASI 190
>NP_001884
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>NP_620693
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>XP_005256394
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>XP_005256393
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GMVRYASV 9
           G  RYAS+
Sbjct: 175 GTARYASI 182
>XP_005247035
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712939
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712938
          Length = 1138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712937
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006712936
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_005247034
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GMVRYAS 8
           G+VR+AS
Sbjct: 785 GLVRFAS 791
>XP_006711353
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           +G++R ASV
Sbjct: 555 KGLIRSASV 563
>XP_005245184
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RGMVRYASV 9
           +G++R ASV
Sbjct: 667 KGLIRSASV 675
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.137    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,524
Number of extensions: 589
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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