Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     869
NP_000240                                                              19     869
XP_005265218                                                           19     869
NP_078966                                                              18    1135
NP_055196                                                              17    4312
NP_001035197                                                           17    4312
XP_005267591                                                           17    4312
XP_005267590                                                           17    4312
XP_005267589                                                           17    4312
XP_005267588                                                           17    4312
XP_006720179                                                           17    4312
NP_001264044                                                           16    5631
NP_004949                                                              16    7355
NP_001161384                                                           16    7355
NP_001161383                                                           16    7355
NP_078897                                                              16    7355
NP_065875                                                              16    7355
NP_006169                                                              16    7355
XP_005276373                                                           16    7355
XP_006725689                                                           16    7355
XP_006725339                                                           16    7355
XP_006721992                                                           16    7355
XP_005253460                                                           16    7355
XP_006719161                                                           16    7355
XP_006719160                                                           16    7355
XP_005252604                                                           16    7355
XP_005252603                                                           16    7355
XP_005252601                                                           16    7355
XP_006717551                                                           16    7355
XP_005252600                                                           16    7355
XP_005252599                                                           16    7355
XP_006717550                                                           16    7355
XP_005263496                                                           16    7355
XP_005263495                                                           16    7355
NP_060558                                                              16    7355
NP_003927                                                              15    9605
NP_714928                                                              15    9605
XP_006713930                                                           15    9605
XP_006713929                                                           15    9605
XP_006713928                                                           15    9605
>NP_001245200
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALASIS
Sbjct: 100 RGEALASIS 108
>NP_000240
          Length = 756

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALASIS
Sbjct: 100 RGEALASIS 108
>XP_005265218
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALASIS
Sbjct: 100 RGEALASIS 108
>NP_078966
          Length = 14507

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    GDALASIS 9
            GDALASIS
Sbjct: 4575 GDALASIS 4582
>NP_055196
          Length = 1429

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>NP_001035197
          Length = 1453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>XP_005267591
          Length = 1275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>XP_005267590
          Length = 1394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>XP_005267589
          Length = 1418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>XP_005267588
          Length = 1453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>XP_006720179
          Length = 1453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+I+
Sbjct: 93  RGEALANIA 101
>NP_001264044
          Length = 4516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GDALASIS 9
            GD LAS+S
Sbjct: 2521 GDTLASLS 2528
>NP_004949
          Length = 2549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RGDALAS 7
            +GDALAS
Sbjct: 1241 QGDALAS 1247
>NP_001161384
          Length = 833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RGD  ASIS
Sbjct: 470 RGDIPASIS 478
>NP_001161383
          Length = 788

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RGD  ASIS
Sbjct: 470 RGDIPASIS 478
>NP_078897
          Length = 833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RGD  ASIS
Sbjct: 470 RGDIPASIS 478
>NP_065875
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>NP_006169
          Length = 744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGDALASI 8
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_005276373
          Length = 487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RG  LAS+S
Sbjct: 199 RGSVLASLS 207
>XP_006725689
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGDALASI 8
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_006725339
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGDALASI 8
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_006721992
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGDALASI 8
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_005253460
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RG  LAS+S
Sbjct: 176 RGSVLASLS 184
>XP_006719161
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RG  LAS+S
Sbjct: 176 RGSVLASLS 184
>XP_006719160
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RG  LAS+S
Sbjct: 195 RGSVLASLS 203
>XP_005252604
          Length = 1386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252603
          Length = 1457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252601
          Length = 1948

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 788 GDSLASI 794
>XP_006717551
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252600
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252599
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_006717550
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GDALASI 8
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005263496
          Length = 2322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RGDALAS 7
            +GDALAS
Sbjct: 1014 QGDALAS 1020
>XP_005263495
          Length = 2549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RGDALAS 7
            +GDALAS
Sbjct: 1241 QGDALAS 1247
>NP_060558
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGDALASIS 9
           RG  LAS+S
Sbjct: 176 RGSVLASLS 184
>NP_003927
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGDALAS 7
           RGD LAS
Sbjct: 248 RGDELAS 254
>NP_714928
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+++
Sbjct: 652 RGNALATLT 660
>XP_006713930
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+++
Sbjct: 652 RGNALATLT 660
>XP_006713929
          Length = 930

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+++
Sbjct: 652 RGNALATLT 660
>XP_006713928
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RGDALASIS 9
           RG+ALA+++
Sbjct: 652 RGNALATLT 660
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.126    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 431,348
Number of extensions: 680
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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