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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     869
NP_000240                                                              19     869
XP_005265218                                                           19     869
NP_001139150                                                           18    1482
NP_775936                                                              18    1482
XP_006714651                                                           18    1482
NP_001073991                                                           16    5631
XP_005248236                                                           16    5631
XP_005248234                                                           16    5631

>NP_001245200
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RFITSKLQS 9
          RF TSKLQS
Sbjct: 79 RFTTSKLQS 87



>NP_000240
          Length = 756

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RFITSKLQS 9
          RF TSKLQS
Sbjct: 79 RFTTSKLQS 87



>XP_005265218
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RFITSKLQS 9
          RF TSKLQS
Sbjct: 79 RFTTSKLQS 87



>NP_001139150
          Length = 1275

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           RF+ SKLQS
Sbjct: 170 RFLVSKLQS 178



>NP_775936
          Length = 324

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           RF+ SKLQS
Sbjct: 170 RFLVSKLQS 178



>XP_006714651
          Length = 1280

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           RF+ SKLQS
Sbjct: 170 RFLVSKLQS 178



>NP_001073991
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           +F+T KLQ+
Sbjct: 448 KFLTDKLQA 456



>XP_005248236
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           +F+T KLQ+
Sbjct: 448 KFLTDKLQA 456



>XP_005248234
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFITSKLQS 9
           +F+T KLQ+
Sbjct: 448 KFLTDKLQA 456



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.129    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 291,260
Number of extensions: 276
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)