Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001175                                                              18    1482
NP_000213                                                              18    1935
NP_001087241                                                           18    1935
NP_004110                                                              18    1935
XP_005265799                                                           18    1935
XP_005265798                                                           18    1935
XP_005265797                                                           18    1935
NP_001265166                                                           17    2528
NP_055069                                                              17    2528
XP_006719258                                                           17    2528
XP_005268620                                                           17    2528
XP_005268619                                                           17    2528
NP_005202                                                              17    4312
NP_001275634                                                           17    4312
NP_005948                                                              15    9605
NP_722577                                                              15    9605
NP_001399                                                              15    9605
NP_001138641                                                           15    9605
NP_001138640                                                           15    9605
XP_005263520                                                           15    9605
XP_005263519                                                           15    9605
XP_005263518                                                           15    9605
XP_005263517                                                           15    9605
XP_005263515                                                           15    9605
XP_006710468                                                           15    9605
XP_005270637                                                           15    9605
>NP_001175
          Length = 2644

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    REALMSVLK 9
            RE LMSVLK
Sbjct: 2547 REPLMSVLK 2555
>NP_000213
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 634 REALMSELK 642
>NP_001087241
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 630 REALMSELK 638
>NP_004110
          Length = 993

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 655 REALMSELK 663
>XP_005265799
          Length = 973

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 631 REALMSELK 639
>XP_005265798
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 635 REALMSELK 643
>XP_005265797
          Length = 977

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           REALMS LK
Sbjct: 635 REALMSELK 643
>NP_001265166
          Length = 183

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  REALMSVLK 9
          R+ALM++LK
Sbjct: 22 RQALMNILK 30
>NP_055069
          Length = 443

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   REALMSVLK 9
           R+ALM++LK
Sbjct: 282 RQALMNILK 290
>XP_006719258
          Length = 183

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  REALMSVLK 9
          R+ALM++LK
Sbjct: 22 RQALMNILK 30
>XP_005268620
          Length = 340

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   REALMSVLK 9
           R+ALM++LK
Sbjct: 179 RQALMNILK 187
>XP_005268619
          Length = 340

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   REALMSVLK 9
           R+ALM++LK
Sbjct: 179 RQALMNILK 187
>NP_005202
          Length = 972

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           +EALMS LK
Sbjct: 627 KEALMSELK 635
>NP_001275634
          Length = 972

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           +EALMS LK
Sbjct: 627 KEALMSELK 635
>NP_005948
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 523 EALLQVLK 530
>NP_722577
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           R+AL+ V+K
Sbjct: 766 RQALLGVIK 774
>NP_001399
          Length = 2923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EALMSVL 8
            EA+MSVL
Sbjct: 1095 EAIMSVL 1101
>NP_001138641
          Length = 997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           R+AL+ V+K
Sbjct: 896 RQALLGVIK 904
>NP_001138640
          Length = 1079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   REALMSVLK 9
           R+AL+ V+K
Sbjct: 965 RQALLGVIK 973
>XP_005263520
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 441 EALLQVLK 448
>XP_005263519
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 523 EALLQVLK 530
>XP_005263518
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 523 EALLQVLK 530
>XP_005263517
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 523 EALLQVLK 530
>XP_005263515
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSVLK 9
           EAL+ VLK
Sbjct: 564 EALLQVLK 571
>XP_006710468
          Length = 2916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EALMSVL 8
            EA+MSVL
Sbjct: 1095 EAIMSVL 1101
>XP_005270637
          Length = 2921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EALMSVL 8
            EA+MSVL
Sbjct: 1095 EAIMSVL 1101
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.129    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 209,768
Number of extensions: 188
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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