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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     509
NP_005913                                                              20     509
XP_005267046                                                           20     509
XP_005267045                                                           20     509
NP_004068                                                              16    7355
NP_004057                                                              15    9605
NP_689935                                                              15    9605
NP_004335                                                              15    9605
NP_056512                                                              15    9605
XP_006726390                                                           15    9605
XP_006726389                                                           15    9605
XP_006726388                                                           15    9605
XP_006726387                                                           15    9605
XP_005271506                                                           15    9605
XP_006718849                                                           15    9605
XP_006711142                                                           15    9605
XP_006711141                                                           15    9605
XP_006711140                                                           15    9605
XP_006710131                                                           15    9605
XP_006710130                                                           15    9605
XP_006710129                                                           15    9605
XP_006710128                                                           15    9605

>NP_006715
          Length = 1558

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RASRALGFA 9
           RAS+ALGFA
Sbjct: 811 RASKALGFA 819



>NP_005913
          Length = 1608

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RASRALGFA 9
           RAS+ALGFA
Sbjct: 811 RASKALGFA 819



>XP_005267046
          Length = 1554

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RASRALGFA 9
           RAS+ALGFA
Sbjct: 811 RASKALGFA 819



>XP_005267045
          Length = 1604

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RASRALGFA 9
           RAS+ALGFA
Sbjct: 811 RASKALGFA 819



>NP_004068
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SRALGF 8
           SRALGF
Sbjct: 439 SRALGF 444



>NP_004057
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  ASRALGF 8
          A RALGF
Sbjct: 56 AMRALGF 62



>NP_689935
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RASRALGFA 9
          RASRAL  A
Sbjct: 10 RASRALRLA 18



>NP_004335
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  ASRALGF 8
          A RALGF
Sbjct: 56 AMRALGF 62



>NP_056512
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  SRALGFA 9
          SRA GFA
Sbjct: 54 SRAAGFA 60



>XP_006726390
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1191 ASRAAGF 1197



>XP_006726389
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1309 ASRAAGF 1315



>XP_006726388
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1327 ASRAAGF 1333



>XP_006726387
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1375 ASRAAGF 1381



>XP_005271506
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RASRALGFA 9
          RASRAL  A
Sbjct: 10 RASRALRLA 18



>XP_006718849
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RASRALGFA 9
          RASRAL  A
Sbjct: 10 RASRALRLA 18



>XP_006711142
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1309 ASRAAGF 1315



>XP_006711141
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1327 ASRAAGF 1333



>XP_006711140
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1375 ASRAAGF 1381



>XP_006710131
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1191 ASRAAGF 1197



>XP_006710130
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1309 ASRAAGF 1315



>XP_006710129
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1327 ASRAAGF 1333



>XP_006710128
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ASRALGF 8
            ASRA GF
Sbjct: 1375 ASRAAGF 1381



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.135    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 288,988
Number of extensions: 248
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)