Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              19     665
NP_066124                                                              19     665
XP_006717999                                                           19     665
NP_005474                                                              17    3301
NP_055730                                                              17    4312
NP_075060                                                              17    4312
NP_001242982                                                           17    4312
NP_001094064                                                           17    4312
NP_060189                                                              17    4312
XP_006717494                                                           17    4312
XP_006717493                                                           17    4312
XP_006717491                                                           17    4312
XP_006717490                                                           17    4312
XP_006717488                                                           17    4312
XP_006717487                                                           17    4312
XP_006717486                                                           17    4312
XP_005265303                                                           17    4312
XP_005265302                                                           17    4312
NP_005531                                                              16    5631
NP_612372                                                              16    5631
XP_006722230                                                           16    5631
XP_006722229                                                           16    5631
XP_005257838                                                           16    5631
XP_005257837                                                           16    5631
XP_005257836                                                           16    5631
XP_005257835                                                           16    5631
XP_005257834                                                           16    5631
XP_006710684                                                           16    5631
XP_006710683                                                           16    5631
XP_006710682                                                           16    5631
XP_005270893                                                           16    5631
XP_005270892                                                           16    5631
XP_005270890                                                           16    5631
NP_004802                                                              16    7355
NP_001137467                                                           16    7355
XP_006718813                                                           16    7355
NP_001121364                                                           15    9605
NP_002959                                                              15    9605
NP_060264                                                              15    9605
NP_001243718                                                           15    9605
NP_001243719                                                           15    9605
NP_005174                                                              15    9605
NP_003117                                                              15    9605
XP_006721304                                                           15    9605
XP_005251551                                                           15    9605
XP_006716857                                                           15    9605
>NP_065681
          Length = 1072

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           R SLDSMEN
Sbjct: 694 RPSLDSMEN 702
>NP_066124
          Length = 1114

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           R SLDSMEN
Sbjct: 694 RPSLDSMEN 702
>XP_006717999
          Length = 1207

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           R SLDSMEN
Sbjct: 787 RPSLDSMEN 795
>NP_005474
          Length = 956

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  ASLDSMEN 9
          ASLD++EN
Sbjct: 70 ASLDTLEN 77
>NP_055730
          Length = 1710

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1639 RASNNSMEN 1647
>NP_075060
          Length = 247

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RASLDSMEN 9
           +  LDSMEN
Sbjct: 183 KIDLDSMEN 191
>NP_001242982
          Length = 1709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1638 RASNNSMEN 1646
>NP_001094064
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           RASL+ +EN
Sbjct: 257 RASLEEIEN 265
>NP_060189
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           RASL+ +EN
Sbjct: 257 RASLEEIEN 265
>XP_006717494
          Length = 1571

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1500 RASNNSMEN 1508
>XP_006717493
          Length = 1610

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1539 RASNNSMEN 1547
>XP_006717491
          Length = 1677

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1606 RASNNSMEN 1614
>XP_006717490
          Length = 1791

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 1720 RASNNSMEN 1728
>XP_006717488
          Length = 2031

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 2001 RASNNSMEN 2009
>XP_006717487
          Length = 2071

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 2000 RASNNSMEN 2008
>XP_006717486
          Length = 2072

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RASLDSMEN 9
            RAS +SMEN
Sbjct: 2001 RASNNSMEN 2009
>XP_005265303
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           RASL+ +EN
Sbjct: 257 RASLEEIEN 265
>XP_005265302
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           RASL+ +EN
Sbjct: 257 RASLEEIEN 265
>NP_005531
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 578 SLDKMEN 584
>NP_612372
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 729 LDSMEN 734
>XP_006722230
          Length = 610

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 517 LDSMEN 522
>XP_006722229
          Length = 610

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 517 LDSMEN 522
>XP_005257838
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 565 LDSMEN 570
>XP_005257837
          Length = 658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 565 LDSMEN 570
>XP_005257836
          Length = 822

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 729 LDSMEN 734
>XP_005257835
          Length = 869

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 777 LDSMEN 782
>XP_005257834
          Length = 870

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LDSMEN 9
           LDSMEN
Sbjct: 777 LDSMEN 782
>XP_006710684
          Length = 794

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 459 SLDKMEN 465
>XP_006710683
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 358 SLDKMEN 364
>XP_006710682
          Length = 720

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 385 SLDKMEN 391
>XP_005270893
          Length = 749

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 414 SLDKMEN 420
>XP_005270892
          Length = 801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 578 SLDKMEN 584
>XP_005270890
          Length = 911

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLDSMEN 9
           SLD MEN
Sbjct: 576 SLDKMEN 582
>NP_004802
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RASLDSM 7
           +ASLDSM
Sbjct: 125 KASLDSM 131
>NP_001137467
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RASLDSM 7
           +ASLDSM
Sbjct: 130 KASLDSM 136
>XP_006718813
          Length = 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  RASLDSM 7
          +ASLDSM
Sbjct: 29 KASLDSM 35
>NP_001121364
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           +ASL S+EN
Sbjct: 785 QASLKSVEN 793
>NP_002959
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           +ASL S+EN
Sbjct: 882 QASLKSVEN 890
>NP_060264
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SLDSMEN 9
            SLD++EN
Sbjct: 1120 SLDALEN 1126
>NP_001243718
          Length = 1966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RASLDSMEN 9
            R +LD M+N
Sbjct: 1919 RLTLDEMDN 1927
>NP_001243719
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RASLDSMEN 9
            R +LD M+N
Sbjct: 1865 RLTLDEMDN 1873
>NP_005174
          Length = 1977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RASLDSMEN 9
            R +LD M+N
Sbjct: 1930 RLTLDEMDN 1938
>NP_003117
          Length = 2419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    RASLDSME 8
            ++SLDS+E
Sbjct: 1423 KSSLDSLE 1430
>XP_006721304
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RASLDSMEN 9
           +ASL S+EN
Sbjct: 882 QASLKSVEN 890
>XP_005251551
          Length = 1801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SLDSMEN 9
            SLD++EN
Sbjct: 1120 SLDALEN 1126
>XP_006716857
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SLDSMEN 9
            SLD++EN
Sbjct: 1153 SLDALEN 1159
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.111    0.262 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 390,601
Number of extensions: 542
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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