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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1482
NP_005913                                                              18    1482
XP_005267046                                                           18    1482
XP_005267045                                                           18    1482
NP_000171                                                              16    7355
XP_006726609                                                           16    7355
XP_005260474                                                           16    7355
XP_006719787                                                           16    7355
XP_006710027                                                           16    7355
NP_057088                                                              16    7355

>NP_006715
          Length = 1558

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           RARIALV N
Sbjct: 556 RARIALVKN 564



>NP_005913
          Length = 1608

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           RARIALV N
Sbjct: 556 RARIALVKN 564



>XP_005267046
          Length = 1554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           RARIALV N
Sbjct: 556 RARIALVKN 564



>XP_005267045
          Length = 1604

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           RARIALV N
Sbjct: 556 RARIALVKN 564



>NP_000171
          Length = 1103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARIALVT 8
           AR+ALVT
Sbjct: 182 ARVALVT 188



>XP_006726609
          Length = 2838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RARIALVT 8
            R R+ALVT
Sbjct: 2452 RCRLALVT 2459



>XP_005260474
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           +AR+A +TN
Sbjct: 364 KARLAQITN 372



>XP_006719787
          Length = 2838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RARIALVT 8
            R R+ALVT
Sbjct: 2452 RCRLALVT 2459



>XP_006710027
          Length = 2837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RARIALVT 8
            R R+ALVT
Sbjct: 2451 RCRLALVT 2458



>NP_057088
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RARIALVTN 9
           +AR+A +TN
Sbjct: 364 KARLAQITN 372



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.134    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 241,887
Number of extensions: 246
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)