Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001263689                                                           20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001263625                                                           20     509
NP_001119590                                                           20     509
NP_001263690                                                           20     509
NP_001119585                                                           20     509
NP_001263624                                                           20     509
NP_001119588                                                           20     509
NP_001119587                                                           20     509
NP_001119589                                                           20     509
NP_055448                                                              17    3301
NP_001108451                                                           16    5631
NP_001108454                                                           16    5631
NP_003713                                                              16    5631
NP_001108450                                                           16    5631
NP_001108452                                                           16    5631
NP_001108453                                                           16    5631
NP_001191121                                                           16    5631
NP_001119713                                                           16    5631
NP_001191117                                                           16    5631
NP_001119712                                                           16    5631
NP_001119714                                                           16    5631
NP_005418                                                              16    5631
NP_001191116                                                           16    5631
NP_001191115                                                           16    5631
NP_001191119                                                           16    5631
NP_001263627                                                           16    5631
NP_001191113                                                           16    5631
NP_001263628                                                           16    5631
NP_001191118                                                           16    5631
NP_001191120                                                           16    5631
NP_001191114                                                           16    5631
NP_001263626                                                           16    5631
XP_005247903                                                           16    5631
XP_005247901                                                           16    5631
XP_005247900                                                           16    5631
NP_061923                                                              16    7355
XP_005263135                                                           16    7355
XP_005263134                                                           16    7355
XP_006714303                                                           16    7355
XP_005263133                                                           16    7355
XP_005263132                                                           16    7355
XP_005263131                                                           16    7355
XP_005263130                                                           16    7355
NP_001013758                                                           15    9605
XP_005250117                                                           15    9605
NP_003583                                                              15    9605
>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263689
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263625
          Length = 302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119590
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001263690
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263624
          Length = 307

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM IYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119588
          Length = 209

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMTIYKQS 9
          RAM IYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_001119587
          Length = 261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMTIYKQS 9
          RAM IYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_001119589
          Length = 214

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMTIYKQS 9
          RAM IYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_055448
          Length = 1173

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAMTIYKQ 8
           RA+ IYKQ
Sbjct: 631 RALAIYKQ 638
>NP_001108451
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 226 RAMPVYKKA 234
>NP_001108454
          Length = 393

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 132 RAMPVYKKA 140
>NP_003713
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 226 RAMPVYKKA 234
>NP_001108450
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 226 RAMPVYKKA 234
>NP_001108452
          Length = 586

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 132 RAMPVYKKA 140
>NP_001108453
          Length = 461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 132 RAMPVYKKA 140
>NP_001191121
          Length = 565

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 105 RAMPVYKKA 113
>NP_001119713
          Length = 450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_001191117
          Length = 540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001119712
          Length = 587

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_001119714
          Length = 426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_005418
          Length = 636

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001191116
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001191115
          Length = 403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001191119
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_001263627
          Length = 182

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MTIYKQS 9
         M IYKQS
Sbjct: 1 MAIYKQS 7
>NP_001191113
          Length = 499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001263628
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MTIYKQS 9
         M IYKQS
Sbjct: 1 MAIYKQS 7
>NP_001191118
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_001191120
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 127 RAMPVYKKA 135
>NP_001191114
          Length = 475

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 176 RAMPVYKKA 184
>NP_001263626
          Length = 234

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MTIYKQS 9
         M IYKQS
Sbjct: 1 MAIYKQS 7
>XP_005247903
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 226 RAMPVYKKA 234
>XP_005247901
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 209 RAMPVYKKA 217
>XP_005247900
          Length = 676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAMTIYKQS 9
           RAM +YK++
Sbjct: 226 RAMPVYKKA 234
>NP_061923
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263135
          Length = 488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263134
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_006714303
          Length = 957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AMTIYKQ 8
          AMT+Y+Q
Sbjct: 40 AMTLYEQ 46
>XP_005263133
          Length = 1022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263132
          Length = 1023

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263131
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>XP_005263130
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMTIYKQ 8
           AMT+Y+Q
Sbjct: 156 AMTLYEQ 162
>NP_001013758
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    RAMTIYK 7
            RAM +YK
Sbjct: 1017 RAMQVYK 1023
>XP_005250117
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTIYKQS 9
           +T+YK+S
Sbjct: 225 LTVYKES 231
>NP_003583
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MTIYKQS 9
           +T+YK+S
Sbjct: 225 LTVYKES 231
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.120    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,467
Number of extensions: 393
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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