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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1935
NP_001263689                                                           18    1935
NP_001119584                                                           18    1935
NP_001119586                                                           18    1935
NP_001263625                                                           18    1935
NP_001119590                                                           18    1935
NP_001263690                                                           18    1935
NP_001119585                                                           18    1935
NP_001263624                                                           18    1935
NP_001119588                                                           18    1935
NP_001119587                                                           18    1935
NP_001119589                                                           18    1935
NP_031392                                                              17    2528
NP_001007268                                                           17    2528
NP_001182570                                                           17    2528
XP_005246450                                                           17    2528
XP_005246449                                                           17    2528
NP_001005497                                                           17    3301
NP_001120936                                                           17    4312
NP_006030                                                              16    5631
NP_859061                                                              16    5631
NP_001005275                                                           16    5631
NP_001001674                                                           16    5631
NP_001004758                                                           16    5631
NP_079042                                                              15    9605
NP_075556                                                              15    9605
XP_006723676                                                           15    9605
XP_006723675                                                           15    9605
XP_006723673                                                           15    9605

>NP_000537
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263689
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119584
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001119586
          Length = 341

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263625
          Length = 302

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119590
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001263690
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119585
          Length = 346

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263624
          Length = 307

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAMAICKQS 9
           RAMAI KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119588
          Length = 209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMAICKQS 9
          RAMAI KQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119587
          Length = 261

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMAICKQS 9
          RAMAI KQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119589
          Length = 214

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RAMAICKQS 9
          RAMAI KQS
Sbjct: 26 RAMAIYKQS 34



>NP_031392
          Length = 1463

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RAMAICKQ 8
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001007268
          Length = 1324

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RAMAICKQ 8
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001182570
          Length = 1461

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RAMAICKQ 8
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>XP_005246450
          Length = 1461

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RAMAICKQ 8
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>XP_005246449
          Length = 1463

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RAMAICKQ 8
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001005497
          Length = 312

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RAMAICK 7
           R MAICK
Sbjct: 120 RCMAICK 126



>NP_001120936
          Length = 3925

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    RAMAICKQS 9
            +A+ +CK+S
Sbjct: 2734 KALGVCKES 2742



>NP_006030
          Length = 1479

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RAMAICKQS 9
           RA  IC+QS
Sbjct: 639 RARYICRQS 647



>NP_859061
          Length = 429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RAMAICKQS 9
           R+  +CKQS
Sbjct: 346 RSCPVCKQS 354



>NP_001005275
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RAMAICK 7
           R MAICK
Sbjct: 150 RYMAICK 156



>NP_001001674
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RAMAICK 7
           R MAICK
Sbjct: 122 RYMAICK 128



>NP_001004758
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RAMAICK 7
           RA+AIC+
Sbjct: 130 RALAICR 136



>NP_079042
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   RAMAICK 7
           RA+A+C+
Sbjct: 146 RALAVCE 152



>NP_075556
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RAMAICKQS 9
            R   +CKQS
Sbjct: 1666 RRRRVCKQS 1674



>XP_006723676
          Length = 1385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RAMAICKQS 9
            R   +CKQS
Sbjct: 1342 RRRRVCKQS 1350



>XP_006723675
          Length = 1623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RAMAICKQS 9
            R   +CKQS
Sbjct: 1580 RRRRVCKQS 1588



>XP_006723673
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RAMAICKQS 9
            R   +CKQS
Sbjct: 1666 RRRRVCKQS 1674



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.121    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 565,950
Number of extensions: 477
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)