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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           18    1482
NP_000242                                                              18    1482
XP_005264389                                                           18    1482
NP_001157871                                                           16    5631
NP_037458                                                              16    7355
NP_001263271                                                           16    7355
XP_006722508                                                           16    7355
NP_003205                                                              15    9605
NP_776152                                                              15    9605
XP_006721522                                                           15    9605
XP_005269575                                                           15    9605
NP_653282                                                              15    9605
NP_001151733                                                           15    9605

>NP_001245210
          Length = 868

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           RALNLFQ S
Sbjct: 242 RALNLFQGS 250



>NP_000242
          Length = 934

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           RALNLFQ S
Sbjct: 308 RALNLFQGS 316



>XP_005264389
          Length = 921

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           RALNLFQ S
Sbjct: 308 RALNLFQGS 316



>NP_001157871
          Length = 793

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           R LNLF+++
Sbjct: 125 RRLNLFEIN 133



>NP_037458
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           R+LN FQ++
Sbjct: 488 RSLNQFQIA 496



>NP_001263271
          Length = 561

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           R+LN FQ++
Sbjct: 440 RSLNQFQIA 448



>XP_006722508
          Length = 609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           R+LN FQ++
Sbjct: 488 RSLNQFQIA 496



>NP_003205
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RALNLFQVS 9
           +A+NL QVS
Sbjct: 110 KAMNLDQVS 118



>NP_776152
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RALNLFQVS 9
           R LNL QVS
Sbjct: 944 RLLNLRQVS 952



>XP_006721522
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LNLFQV 8
           LNLFQ+
Sbjct: 264 LNLFQI 269



>XP_005269575
          Length = 1113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RALNLFQVS 9
           R LNL QVS
Sbjct: 903 RLLNLRQVS 911



>NP_653282
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LNLFQV 8
           LNLFQ+
Sbjct: 264 LNLFQI 269



>NP_001151733
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LNLFQV 8
           LNLFQ+
Sbjct: 190 LNLFQI 195



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.138    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,768
Number of extensions: 366
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)