Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_958441                                                              20     509
NP_958439                                                              20     509
NP_958440                                                              20     509
NP_005219                                                              20     509
NP_000910                                                              18    1482
XP_006714702                                                           18    1482
XP_006714698                                                           18    1482
XP_006714696                                                           18    1482
XP_006714695                                                           18    1482
XP_006726843                                                           18    1935
XP_006726842                                                           18    1935
XP_006726841                                                           18    1935
XP_005259508                                                           18    1935
XP_006723586                                                           18    1935
XP_006723585                                                           18    1935
XP_006710113                                                           18    1935
XP_003960942                                                           18    1935
XP_006710112                                                           18    1935
XP_005275906                                                           18    1935
NP_000535                                                              17    4312
NP_061313                                                              17    4312
NP_001276972                                                           17    4312
NP_071913                                                              16    7355
NP_001008744                                                           16    7355
NP_060789                                                              16    7355
NP_653328                                                              16    7355
XP_006723533                                                           16    7355
XP_006723532                                                           16    7355
XP_005259458                                                           16    7355
XP_006720264                                                           16    7355
XP_006720263                                                           16    7355
XP_006720262                                                           16    7355
XP_006720261                                                           16    7355
XP_005267905                                                           16    7355
XP_005267904                                                           16    7355
XP_006720260                                                           16    7355
NP_612383                                                              16    7355
NP_005769                                                              15    9605
NP_001243224                                                           15    9605
NP_055129                                                              15    9605
NP_005230                                                              15    9605
NP_689914                                                              15    9605
NP_057078                                                              15    9605
NP_149107                                                              15    9605
XP_005260992                                                           15    9605
XP_005256420                                                           15    9605
XP_005256419                                                           15    9605
XP_005256417                                                           15    9605
XP_005256416                                                           15    9605
XP_005256415                                                           15    9605
XP_005256414                                                           15    9605
XP_005256413                                                           15    9605
XP_005256412                                                           15    9605
XP_005256411                                                           15    9605
XP_005256410                                                           15    9605
XP_005256409                                                           15    9605
XP_005256408                                                           15    9605
XP_005256407                                                           15    9605
XP_005268266                                                           15    9605
XP_006720366                                                           15    9605
XP_005268265                                                           15    9605
XP_005253535                                                           15    9605
XP_006712983                                                           15    9605
XP_006712982                                                           15    9605
XP_006712981                                                           15    9605
XP_006712980                                                           15    9605
NP_997657                                                              15    9605
NP_004118                                                              15    9605
NP_002622                                                              15    9605
>NP_958441
          Length = 705

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           RA GADSYE
Sbjct: 309 RACGADSYE 317
>NP_958439
          Length = 628

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           RA GADSYE
Sbjct: 309 RACGADSYE 317
>NP_958440
          Length = 405

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           RA GADSYE
Sbjct: 309 RACGADSYE 317
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           RA GADSYE
Sbjct: 309 RACGADSYE 317
>NP_000910
          Length = 974

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RAFGADS 7
           RAFGADS
Sbjct: 893 RAFGADS 899
>XP_006714702
          Length = 867

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RAFGADS 7
           RAFGADS
Sbjct: 786 RAFGADS 792
>XP_006714698
          Length = 973

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RAFGADS 7
           RAFGADS
Sbjct: 892 RAFGADS 898
>XP_006714696
          Length = 974

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RAFGADS 7
           RAFGADS
Sbjct: 893 RAFGADS 899
>XP_006714695
          Length = 974

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RAFGADS 7
           RAFGADS
Sbjct: 893 RAFGADS 899
>XP_006726843
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006726842
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006726841
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_005259508
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006723586
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006723585
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006710113
          Length = 742

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 665 KAFGRDSY 672
>XP_003960942
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_006710112
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>XP_005275906
          Length = 718

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DSY
Sbjct: 641 KAFGRDSY 648
>NP_000535
          Length = 703

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           R+FGA+ +E
Sbjct: 343 RSFGAEEHE 351
>NP_061313
          Length = 653

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           R+FGA+ +E
Sbjct: 343 RSFGAEEHE 351
>NP_001276972
          Length = 686

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           R+FGA+ +E
Sbjct: 343 RSFGAEEHE 351
>NP_071913
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RAFGADSY 8
           RAF  DSY
Sbjct: 176 RAFHQDSY 183
>NP_001008744
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>NP_060789
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>NP_653328
          Length = 182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RAFGADSYE 9
           R+FGA+ ++
Sbjct: 144 RSFGAEDFQ 152
>XP_006723533
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DS+
Sbjct: 782 KAFGRDSH 789
>XP_006723532
          Length = 774

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DS+
Sbjct: 586 KAFGRDSH 593
>XP_005259458
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DS+
Sbjct: 782 KAFGRDSH 789
>XP_006720264
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 282 AFGLDSFK 289
>XP_006720263
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 282 AFGLDSFK 289
>XP_006720262
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 282 AFGLDSFK 289
>XP_006720261
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>XP_005267905
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>XP_005267904
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>XP_006720260
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AFGADSYE 9
           AFG DS++
Sbjct: 547 AFGLDSFK 554
>NP_612383
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RAFGADSY 8
           +AFG DS+
Sbjct: 782 KAFGRDSH 789
>NP_005769
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADSY+
Sbjct: 795 GADSYK 800
>NP_001243224
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADS+E
Sbjct: 422 GADSFE 427
>NP_055129
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R FG   YE
Sbjct: 502 REFGTQKYE 510
>NP_005230
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADS+E
Sbjct: 282 GADSFE 287
>NP_689914
          Length = 5058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3    FGADSY 8
            FG DSY
Sbjct: 4640 FGIDSY 4645
>NP_057078
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   GADSYE 9
           G DSYE
Sbjct: 115 GTDSYE 120
>NP_149107
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AFGADSY 8
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>XP_005260992
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADS+E
Sbjct: 282 GADSFE 287
>XP_005256420
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1  RAFGADSYE 9
          R F  D YE
Sbjct: 84 RTFNVDMYE 92
>XP_005256419
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1  RAFGADSYE 9
          R F  D YE
Sbjct: 68 RTFNVDMYE 76
>XP_005256417
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1  RAFGADSYE 9
          R F  D YE
Sbjct: 84 RTFNVDMYE 92
>XP_005256416
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1  RAFGADSYE 9
          R F  D YE
Sbjct: 84 RTFNVDMYE 92
>XP_005256415
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256414
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256413
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256412
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256411
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 151 RTFNVDMYE 159
>XP_005256410
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256409
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005256408
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 151 RTFNVDMYE 159
>XP_005256407
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 152 RTFNVDMYE 160
>XP_005268266
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AFGADSY 8
           AFG+D Y
Sbjct: 331 AFGSDYY 337
>XP_006720366
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AFGADSY 8
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>XP_005268265
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AFGADSY 8
           AFG+D Y
Sbjct: 449 AFGSDYY 455
>XP_005253535
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RAFGADSY 8
           R FG +SY
Sbjct: 554 RVFGKNSY 561
>XP_006712983
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712982
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712981
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADSY+
Sbjct: 548 GADSYK 553
>XP_006712980
          Length = 756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GADSYE 9
           GADSY+
Sbjct: 736 GADSYK 741
>NP_997657
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   RAFGADSYE 9
           R F  D YE
Sbjct: 124 RTFNVDMYE 132
>NP_004118
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1  RAFGADSYE 9
          R F  D YE
Sbjct: 84 RTFNVDMYE 92
>NP_002622
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FGADSYE 9
           FGA +YE
Sbjct: 450 FGAHTYE 456
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.133    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 598,815
Number of extensions: 1277
Number of successful extensions: 69
Number of sequences better than 10000.0: 69
Number of HSP's gapped: 69
Number of HSP's successfully gapped: 69
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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