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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     299
NP_065916                                                              17    3301
NP_001107869                                                           17    3301
NP_000370                                                              17    4312
NP_004516                                                              16    5631
NP_037512                                                              16    5631
NP_001076804                                                           16    5631
XP_006712591                                                           16    5631
XP_006712589                                                           16    5631
NP_003473                                                              16    7355
NP_002326                                                              16    7355
NP_002327                                                              16    7355
XP_006719679                                                           16    7355
XP_005269219                                                           16    7355
XP_006719678                                                           16    7355
XP_006719677                                                           16    7355
XP_006719141                                                           16    7355
XP_005274051                                                           16    7355
NP_001177766                                                           15    9605
NP_001128512                                                           15    9605
NP_004073                                                              15    9605
NP_075408                                                              15    9605
NP_001128513                                                           15    9605
NP_001177765                                                           15    9605
NP_473449                                                              15    9605
NP_001140201                                                           15    9605

>NP_004251
          Length = 1208

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QVQRAFMQG 9
           +VQRAFMQG
Sbjct: 755 RVQRAFMQG 763



>NP_065916
          Length = 1008

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           Q +R FMQG
Sbjct: 417 QFKRGFMQG 425



>NP_001107869
          Length = 994

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           Q +R FMQG
Sbjct: 417 QFKRGFMQG 425



>NP_000370
          Length = 1333

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QVQRAFMQG 9
            QV+ AF+QG
Sbjct: 1195 QVEGAFVQG 1203



>NP_004516
          Length = 4655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVQRAFMQG 9
           +++RAFM G
Sbjct: 537 KLERAFMDG 545



>NP_037512
          Length = 907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QRAFMQG 9
           QRA++QG
Sbjct: 726 QRAYLQG 732



>NP_001076804
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QRAFMQG 9
           QRA++QG
Sbjct: 732 QRAYLQG 738



>XP_006712591
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVQRAFMQG 9
           +++RAFM G
Sbjct: 537 KLERAFMDG 545



>XP_006712589
          Length = 4660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVQRAFMQG 9
           +++RAFM G
Sbjct: 537 KLERAFMDG 545



>NP_003473
          Length = 5537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VQRAFMQG 9
            +QR F+QG
Sbjct: 1921 LQRPFLQG 1928



>NP_002326
          Length = 1615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VQRAFMQG 9
           + RAFM G
Sbjct: 699 ISRAFMNG 706




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           ++ RAFM G
Sbjct: 785 RIVRAFMDG 793



>NP_002327
          Length = 1613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VQRAFMQG 9
           + RAFM G
Sbjct: 686 ISRAFMNG 693



>XP_006719679
          Length = 5536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VQRAFMQG 9
            +QR F+QG
Sbjct: 1920 LQRPFLQG 1927



>XP_005269219
          Length = 5537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VQRAFMQG 9
            +QR F+QG
Sbjct: 1921 LQRPFLQG 1928



>XP_006719678
          Length = 5539

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VQRAFMQG 9
            +QR F+QG
Sbjct: 1923 LQRPFLQG 1930



>XP_006719677
          Length = 5540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VQRAFMQG 9
            +QR F+QG
Sbjct: 1924 LQRPFLQG 1931



>XP_006719141
          Length = 1613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VQRAFMQG 9
           + RAFM G
Sbjct: 686 ISRAFMNG 693



>XP_005274051
          Length = 1653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VQRAFMQG 9
           + RAFM G
Sbjct: 699 ISRAFMNG 706




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           ++ RAFM G
Sbjct: 785 RIVRAFMDG 793



>NP_001177766
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 761 RAFLQG 766



>NP_001128512
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 748 RAFLQG 753



>NP_004073
          Length = 1278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 768 RAFLQG 773



>NP_075408
          Length = 1144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 634 RAFLQG 639



>NP_001128513
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 634 RAFLQG 639



>NP_001177765
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAFMQG 9
           RAF+QG
Sbjct: 731 RAFLQG 736



>NP_473449
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           QV+ A M+G
Sbjct: 249 QVEHALMEG 257



>NP_001140201
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVQRAFMQG 9
           QV+ A M+G
Sbjct: 239 QVEHALMEG 247



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.134    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,242
Number of extensions: 473
Number of successful extensions: 28
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)