Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              20     390
NP_689804                                                              17    2528
NP_001273173                                                           17    2528
NP_001273172                                                           17    2528
XP_006719326                                                           17    2528
XP_006719325                                                           17    2528
NP_055858                                                              17    4312
NP_942568                                                              17    4312
NP_001005489                                                           17    4312
NP_061756                                                              17    4312
NP_000087                                                              16    5631
NP_055320                                                              16    5631
NP_057160                                                              16    5631
XP_006713565                                                           16    5631
XP_006713564                                                           16    5631
XP_006713563                                                           16    5631
XP_006713562                                                           16    5631
NP_058431                                                              16    7355
NP_005179                                                              16    7355
NP_005896                                                              16    7355
NP_001120689                                                           16    7355
XP_005266461                                                           16    7355
XP_005266460                                                           16    7355
XP_005266458                                                           16    7355
XP_006719890                                                           16    7355
NP_689644                                                              16    7355
NP_001238831                                                           15    9605
NP_003976                                                              15    9605
NP_001124430                                                           15    9605
NP_001103132                                                           15    9605
NP_001436                                                              15    9605
NP_002820                                                              15    9605
NP_001138840                                                           15    9605
XP_006717854                                                           15    9605
XP_006717263                                                           15    9605
XP_006717262                                                           15    9605
XP_006717261                                                           15    9605
XP_006717260                                                           15    9605
XP_006714893                                                           15    9605
XP_006714892                                                           15    9605
XP_006714891                                                           15    9605
NP_001035532                                                           15    9605
>NP_005350
          Length = 552

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QVGETFKV 8
           QVGETFKV
Sbjct: 334 QVGETFKV 341
>NP_689804
          Length = 563

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           Q+GET KVT
Sbjct: 431 QLGETGKVT 439
>NP_001273173
          Length = 484

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           Q+GET KVT
Sbjct: 352 QLGETGKVT 360
>NP_001273172
          Length = 523

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           Q+GET KVT
Sbjct: 391 QLGETGKVT 399
>XP_006719326
          Length = 539

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           Q+GET KVT
Sbjct: 431 QLGETGKVT 439
>XP_006719325
          Length = 563

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           Q+GET KVT
Sbjct: 431 QLGETGKVT 439
>NP_055858
          Length = 1233

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VGETFKV 8
           +GETFK+
Sbjct: 234 IGETFKL 240
>NP_942568
          Length = 1250

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VGETFKV 8
           +GETFK+
Sbjct: 234 IGETFKL 240
>NP_001005489
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   QVGETFKVT 9
           Q+G+TF+++
Sbjct: 157 QIGDTFRLS 165
>NP_061756
          Length = 798

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   QVGETFKV 8
           ++G+TFK+
Sbjct: 288 EIGKTFKI 295
>NP_000087
          Length = 1065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VG+T +VT
Sbjct: 456 EVGDTIRVT 464
>NP_055320
          Length = 210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QVGETFKVT 9
          Q+GE F+ T
Sbjct: 61 QIGEVFECT 69
>NP_057160
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  QVGETFK 7
          ++GETFK
Sbjct: 62 ELGETFK 68
>XP_006713565
          Length = 901

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VG+T +VT
Sbjct: 456 EVGDTIRVT 464
>XP_006713564
          Length = 1065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VG+T +VT
Sbjct: 456 EVGDTIRVT 464
>XP_006713563
          Length = 1069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VG+T +VT
Sbjct: 456 EVGDTIRVT 464
>XP_006713562
          Length = 1090

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VG+T +VT
Sbjct: 456 EVGDTIRVT 464
>NP_058431
          Length = 304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QVGETFKVT 9
           +VGE  KVT
Sbjct: 259 EVGELVKVT 267
>NP_005179
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   GETFKVT 9
           G+TF++T
Sbjct: 176 GDTFRIT 182
>NP_005896
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 247 RVGETFQAS 255
>NP_001120689
          Length = 467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 284 RVGETFQAS 292
>XP_005266461
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 161 RVGETFQAS 169
>XP_005266460
          Length = 393

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 198 RVGETFQAS 206
>XP_005266458
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 247 RVGETFQAS 255
>XP_006719890
          Length = 479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVGETFKVT 9
           +VGETF+ +
Sbjct: 284 RVGETFQAS 292
>NP_689644
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QVGETFKVT 9
           Q GET K+T
Sbjct: 180 QRGETIKMT 188
>NP_001238831
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QVGETFKV 8
           Q G TFKV
Sbjct: 425 QNGRTFKV 432
>NP_003976
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QVGETFKV 8
           Q G TFKV
Sbjct: 420 QNGRTFKV 427
>NP_001124430
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GETFKVT 9
           G+TFK T
Sbjct: 126 GKTFKAT 132
>NP_001103132
          Length = 4284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VGETFK 7
            +GETFK
Sbjct: 1232 MGETFK 1237
>NP_001436
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  GETFKVT 9
          G+TFK T
Sbjct: 77 GKTFKAT 83
>NP_002820
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 39 VVQTFKVT 46
>NP_001138840
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 39 VVQTFKVT 46
>XP_006717854
          Length = 2852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    VGETFKV 8
            VGE FKV
Sbjct: 2831 VGEGFKV 2837
>XP_006717263
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 56 VVQTFKVT 63
>XP_006717262
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 39 VVQTFKVT 46
>XP_006717261
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 39 VVQTFKVT 46
>XP_006717260
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGETFKVT 9
          V +TFKVT
Sbjct: 50 VVQTFKVT 57
>XP_006714893
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  GETFKVT 9
          G+TFK T
Sbjct: 77 GKTFKAT 83
>XP_006714892
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GETFKVT 9
           G+TFK T
Sbjct: 98  GKTFKAT 104
>XP_006714891
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GETFKVT 9
           G+TFK T
Sbjct: 126 GKTFKAT 132
>NP_001035532
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GETFKVT 9
           G+TFK T
Sbjct: 126 GKTFKAT 132
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.129    0.342 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 509,844
Number of extensions: 783
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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