Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              20     299
XP_006718908                                                           20     299
XP_005271621                                                           20     299
XP_006718907                                                           20     299
XP_006718906                                                           20     299
XP_005271619                                                           20     299
XP_005271618                                                           20     299
NP_000479                                                              17    2528
XP_005245255                                                           17    2528
NP_001106954                                                           16    5631
NP_001026910                                                           16    5631
NP_149043                                                              16    7355
NP_115492                                                              16    7355
NP_003889                                                              16    7355
NP_001014402                                                           16    7355
NP_008922                                                              16    7355
NP_001020026                                                           16    7355
NP_998761                                                              16    7355
XP_005266631                                                           16    7355
XP_006715655                                                           16    7355
XP_006715654                                                           16    7355
XP_005264840                                                           16    7355
NP_055524                                                              15    9605
NP_000951                                                              15    9605
XP_003403541                                                           15    9605
XP_003846688                                                           15    9605

>NP_000042
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2714 QLVKGRDDL 2722



>XP_006718908
          Length = 1708

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 1366 QLVKGRDDL 1374



>XP_005271621
          Length = 2708

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2366 QLVKGRDDL 2374



>XP_006718907
          Length = 3050

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2708 QLVKGRDDL 2716



>XP_006718906
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2714 QLVKGRDDL 2722



>XP_005271619
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2714 QLVKGRDDL 2722



>XP_005271618
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            QL KGRDDL
Sbjct: 2714 QLVKGRDDL 2722



>NP_000479
          Length = 464

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LAKGRDDL 9
           +A+GRDDL
Sbjct: 387 VAEGRDDL 394



>XP_005245255
          Length = 416

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LAKGRDDL 9
           +A+GRDDL
Sbjct: 339 VAEGRDDL 346



>NP_001106954
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KGRDDL 9
           KGRDD+
Sbjct: 229 KGRDDI 234



>NP_001026910
          Length = 250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KGRDDL 9
           KGRDD+
Sbjct: 229 KGRDDI 234



>NP_149043
          Length = 1018

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AKGRDDL 9
           A+G+DDL
Sbjct: 933 ARGQDDL 939



>NP_115492
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AKGRDD 8
           A+GRDD
Sbjct: 158 ARGRDD 163



>NP_003889
          Length = 1496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AKGRDD 8
          A+GRDD
Sbjct: 25 ARGRDD 30



>NP_001014402
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AKGRDD 8
           A+GRDD
Sbjct: 158 ARGRDD 163



>NP_008922
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  LAKGRDD 8
          L KG+DD
Sbjct: 16 LVKGKDD 22



>NP_001020026
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  LAKGRDD 8
          L KG+DD
Sbjct: 16 LVKGKDD 22



>NP_998761
          Length = 517

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QLAKGRDDL 9
          Q+A GR DL
Sbjct: 59 QMASGRVDL 67



>XP_005266631
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AKGRDD 8
           A+GRDD
Sbjct: 158 ARGRDD 163



>XP_006715655
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AKGRDD 8
          A+GRDD
Sbjct: 25 ARGRDD 30



>XP_006715654
          Length = 1495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  AKGRDD 8
          A+GRDD
Sbjct: 25 ARGRDD 30



>XP_005264840
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  LAKGRDD 8
          L KG+DD
Sbjct: 16 LVKGKDD 22



>NP_055524
          Length = 3546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    QLAKGRDDL 9
            + +KG+DD+
Sbjct: 3125 ETSKGKDDV 3133



>NP_000951
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QLAKGRDD 8
           QLA GR D
Sbjct: 325 QLASGRRD 332



>XP_003403541
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  LAKGRDD 8
          LAK RDD
Sbjct: 49 LAKERDD 55



>XP_003846688
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  LAKGRDD 8
          LAK RDD
Sbjct: 49 LAKERDD 55



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.140    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 591,593
Number of extensions: 689
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)