Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     390
NP_671714                                                              20     390
NP_006022                                                              20     390
XP_005261186                                                           20     390
XP_005261185                                                           20     390
XP_005261184                                                           20     390
XP_005261183                                                           20     390
XP_005261182                                                           20     390
XP_005261181                                                           20     390
XP_006715890                                                           20     390
XP_005250160                                                           20     390
XP_005250159                                                           20     390
NP_003869                                                              17    4312
NP_803173                                                              17    4312
NP_001278017                                                           16    5631
NP_114157                                                              16    5631
NP_055073                                                              16    5631
XP_006723744                                                           16    5631
XP_005260304                                                           16    5631
XP_006723474                                                           16    5631
XP_005259362                                                           16    5631
NP_055105                                                              16    7355
NP_036419                                                              16    7355
NP_085152                                                              16    7355
XP_005256935                                                           16    7355
XP_006717088                                                           16    7355
XP_006713621                                                           16    7355
XP_006713620                                                           16    7355
XP_005244773                                                           16    7355
XP_005244772                                                           16    7355
XP_005244771                                                           16    7355
XP_006710392                                                           16    7355
NP_055531                                                              16    7355
NP_001269356                                                           15    9605
NP_001269355                                                           15    9605
NP_002640                                                              15    9605
NP_001264044                                                           15    9605
NP_001263419                                                           15    9605
NP_775745                                                              15    9605
NP_694995                                                              15    9605
NP_001186224                                                           15    9605
NP_002631                                                              15    9605
NP_001257696                                                           15    9605
NP_001257695                                                           15    9605
NP_942130                                                              15    9605
XP_006723191                                                           15    9605
XP_006716090                                                           15    9605
XP_005250500                                                           15    9605
>NP_005742
          Length = 3907

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3712 RKALIYQKK 3720
>NP_671714
          Length = 3899

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3704 RKALIYQKK 3712
>NP_006022
          Length = 3336

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3146 RKALIYQKK 3154
>XP_005261186
          Length = 3139

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 2949 RKALIYQKK 2957
>XP_005261185
          Length = 3229

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3039 RKALIYQKK 3047
>XP_005261184
          Length = 3258

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3157 RKALIYQKK 3165
>XP_005261183
          Length = 3268

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3078 RKALIYQKK 3086
>XP_005261182
          Length = 3346

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3156 RKALIYQKK 3164
>XP_005261181
          Length = 3347

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3157 RKALIYQKK 3165
>XP_006715890
          Length = 3860

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3665 RKALIYQKK 3673
>XP_005250160
          Length = 3939

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3744 RKALIYQKK 3752
>XP_005250159
          Length = 3952

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKALIYQKK 9
            +KALIYQKK
Sbjct: 3757 RKALIYQKK 3765
>NP_003869
          Length = 318

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QKALIYQ 7
           +KALIYQ
Sbjct: 293 EKALIYQ 299
>NP_803173
          Length = 276

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QKALIYQKK 9
           Q+A  YQKK
Sbjct: 238 QRAAYYQKK 246
>NP_001278017
          Length = 2912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KALIYQKK 9
            K ++YQKK
Sbjct: 1869 KMILYQKK 1876
>NP_114157
          Length = 2873

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KALIYQKK 9
            K ++YQKK
Sbjct: 1830 KMILYQKK 1837
>NP_055073
          Length = 1530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QKALIYQKK 9
           +++LIY+KK
Sbjct: 734 EESLIYRKK 742
>XP_006723744
          Length = 847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QKALIYQKK 9
           +++LIY+KK
Sbjct: 734 EESLIYRKK 742
>XP_005260304
          Length = 1530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QKALIYQKK 9
           +++LIY+KK
Sbjct: 734 EESLIYRKK 742
>XP_006723474
          Length = 2737

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KALIYQKK 9
            K ++YQKK
Sbjct: 1694 KMILYQKK 1701
>XP_005259362
          Length = 2855

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KALIYQKK 9
            K ++YQKK
Sbjct: 1812 KMILYQKK 1819
>NP_055105
          Length = 1098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKALIYQK 8
           QKA++Y K
Sbjct: 398 QKAILYAK 405
>NP_036419
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 296 LVYQKK 301
>NP_085152
          Length = 834

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 298 LVYQKK 303
>XP_005256935
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 221 LVYQKK 226
>XP_006717088
          Length = 1024

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKALIYQK 8
           QKA++Y K
Sbjct: 324 QKAILYAK 331
>XP_006713621
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 303 LVYQKK 308
>XP_006713620
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 303 LVYQKK 308
>XP_005244773
          Length = 792

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 256 LVYQKK 261
>XP_005244772
          Length = 801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 298 LVYQKK 303
>XP_005244771
          Length = 802

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 266 LVYQKK 271
>XP_006710392
          Length = 834

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 298 LVYQKK 303
>NP_055531
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 295 LVYQKK 300
>NP_001269356
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 98  LLYQKK 103
>NP_001269355
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 98  LLYQKK 103
>NP_002640
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 98  LLYQKK 103
>NP_001264044
          Length = 4516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALIYQK 8
           AL+YQK
Sbjct: 666 ALVYQK 671
>NP_001263419
          Length = 192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKALIYQK 8
          +KAL Y+K
Sbjct: 60 EKALTYEK 67
>NP_775745
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KALIYQK 8
           KAL+Y+K
Sbjct: 290 KALLYKK 296
>NP_694995
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKALIYQKK 9
           +KA IY+ K
Sbjct: 578 EKAFIYKNK 586
>NP_001186224
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKALIYQKK 9
           +KA IY+ K
Sbjct: 591 EKAFIYKNK 599
>NP_002631
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKALIYQK 8
           +KAL Y+K
Sbjct: 242 EKALTYEK 249
>NP_001257696
          Length = 205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KALIYQK 8
           KAL+Y+K
Sbjct: 185 KALLYKK 191
>NP_001257695
          Length = 205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KALIYQK 8
           KAL+Y+K
Sbjct: 185 KALLYKK 191
>NP_942130
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKALIYQK 8
           +KAL Y+K
Sbjct: 242 EKALTYEK 249
>XP_006723191
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKALIYQKK 9
           +KA IY+ K
Sbjct: 569 EKAFIYKNK 577
>XP_006716090
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 98  LLYQKK 103
>XP_005250500
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LIYQKK 9
           L+YQKK
Sbjct: 98  LLYQKK 103
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.128    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 470,604
Number of extensions: 677
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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