Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001273013                                                           18    1135
XP_003960788                                                           18    1135
XP_005257969                                                           18    1135
XP_005275882                                                           18    1135
NP_003368                                                              17    3301
NP_001230662                                                           17    3301
NP_055990                                                              17    4312
NP_061130                                                              17    4312
XP_005265049                                                           17    4312
XP_006713135                                                           17    4312
NP_940857                                                              17    4312
NP_001243437                                                           16    5631
NP_055699                                                              16    5631
NP_004678                                                              16    5631
NP_001017392                                                           16    5631
XP_006722231                                                           16    5631
XP_005257843                                                           16    5631
XP_005257842                                                           16    5631
XP_005257841                                                           16    5631
XP_006718608                                                           16    5631
NP_057735                                                              16    7355
NP_001275892                                                           16    7355
XP_006723003                                                           16    7355
NP_001137408                                                           16    7355
NP_689856                                                              15    9605
NP_001073339                                                           15    9605
NP_001073340                                                           15    9605
NP_817084                                                              15    9605
NP_001229437                                                           15    9605
NP_542164                                                              15    9605
XP_006722446                                                           15    9605
XP_006722445                                                           15    9605
XP_006722444                                                           15    9605
XP_006722443                                                           15    9605
XP_006718267                                                           15    9605
XP_006718266                                                           15    9605
XP_006718265                                                           15    9605
XP_006718258                                                           15    9605
XP_006718257                                                           15    9605
>NP_001273013
          Length = 106

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ASRATCRP 9
           ASRATC P
Sbjct: 98  ASRATCLP 105
>XP_003960788
          Length = 279

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QASRATCRP 9
           +A +ATCRP
Sbjct: 115 RAPKATCRP 123
>XP_005257969
          Length = 279

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QASRATCRP 9
           +A +ATCRP
Sbjct: 115 RAPKATCRP 123
>XP_005275882
          Length = 280

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QASRATCRP 9
           +A +ATCRP
Sbjct: 115 RAPKATCRP 123
>NP_003368
          Length = 207

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SRATCRP 9
          +RATC+P
Sbjct: 43 TRATCQP 49
>NP_001230662
          Length = 188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SRATCRP 9
          +RATC+P
Sbjct: 43 TRATCQP 49
>NP_055990
          Length = 2754

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ASRATC 7
           ASRATC
Sbjct: 484 ASRATC 489
>NP_061130
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   ASRATCRP 9
           ASRA C P
Sbjct: 195 ASRAACDP 202
>XP_005265049
          Length = 2720

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ASRATC 7
           ASRATC
Sbjct: 450 ASRATC 455
>XP_006713135
          Length = 2727

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ASRATC 7
           ASRATC
Sbjct: 457 ASRATC 462
>NP_940857
          Length = 5147

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    ASRATCRP 9
            ASRA C P
Sbjct: 2459 ASRALCSP 2466
>NP_001243437
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 172 SRATARP 178
>NP_055699
          Length = 1082

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QASRATCRP 9
           QAS  TC P
Sbjct: 941 QASSGTCFP 949
>NP_004678
          Length = 1195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 115 SRATARP 121
>NP_001017392
          Length = 1082

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   QASRATCRP 9
           QAS  TC P
Sbjct: 941 QASSGTCFP 949
>XP_006722231
          Length = 1195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 115 SRATARP 121
>XP_005257843
          Length = 1195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 115 SRATARP 121
>XP_005257842
          Length = 1199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 119 SRATARP 125
>XP_005257841
          Length = 1209

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 129 SRATARP 135
>XP_006718608
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           SRAT RP
Sbjct: 172 SRATARP 178
>NP_057735
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QASRATCR 8
           QAS A CR
Sbjct: 227 QASGAVCR 234
>NP_001275892
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           +RATC P
Sbjct: 541 ARATCPP 547
>XP_006723003
          Length = 649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SRATCRP 9
           +RATC P
Sbjct: 597 ARATCPP 603
>NP_001137408
          Length = 157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  ASRATCRP 9
          ASRA  RP
Sbjct: 21 ASRAAARP 28
>NP_689856
          Length = 1749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    ASRATCRP 9
            +SRA C P
Sbjct: 1007 SSRAPCSP 1014
>NP_001073339
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ASRATCR 8
           ASR +CR
Sbjct: 592 ASRTSCR 598
>NP_001073340
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ASRATCR 8
           ASR +CR
Sbjct: 584 ASRTSCR 590
>NP_817084
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ASRATCR 8
           ASR +CR
Sbjct: 609 ASRTSCR 615
>NP_001229437
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          +ASR TC
Sbjct: 24 EASRETC 30
>NP_542164
          Length = 950

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QASRATC 7
           +ASR TC
Sbjct: 406 EASRETC 412
>XP_006722446
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QASRATC 7
           +ASR TC
Sbjct: 406 EASRETC 412
>XP_006722445
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QASRATC 7
           +ASR TC
Sbjct: 406 EASRETC 412
>XP_006722444
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QASRATC 7
           +ASR TC
Sbjct: 406 EASRETC 412
>XP_006722443
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QASRATC 7
           +ASR TC
Sbjct: 381 EASRETC 387
>XP_006718267
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          + SRATC
Sbjct: 46 RVSRATC 52
>XP_006718266
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          + SRATC
Sbjct: 46 RVSRATC 52
>XP_006718265
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          + SRATC
Sbjct: 46 RVSRATC 52
>XP_006718258
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          + SRATC
Sbjct: 46 RVSRATC 52
>XP_006718257
          Length = 627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QASRATC 7
          + SRATC
Sbjct: 46 RVSRATC 52
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.118    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 493,863
Number of extensions: 378
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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