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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1935
NP_005913                                                              18    1935
XP_005267046                                                           18    1935
XP_005267045                                                           18    1935
NP_064569                                                              16    7355
XP_005263972                                                           16    7355
XP_005263971                                                           16    7355
NP_001229295                                                           15    9605
NP_542400                                                              15    9605
XP_005277843                                                           15    9605

>NP_006715
          Length = 1558

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSLLSIK L
Sbjct: 643 PSLLSIKQL 651



>NP_005913
          Length = 1608

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSLLSIK L
Sbjct: 643 PSLLSIKQL 651



>XP_005267046
          Length = 1554

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSLLSIK L
Sbjct: 643 PSLLSIKQL 651



>XP_005267045
          Length = 1604

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSLLSIK L
Sbjct: 643 PSLLSIKQL 651



>NP_064569
          Length = 775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSL+S K+L
Sbjct: 567 PSLISEKIL 575



>XP_005263972
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSL+S K+L
Sbjct: 567 PSLISEKIL 575



>XP_005263971
          Length = 796

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   PSLLSIKLL 9
           PSL+S K+L
Sbjct: 567 PSLISEKIL 575



>NP_001229295
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLLSIKLL 9
           SLL++KLL
Sbjct: 318 SLLALKLL 325



>NP_542400
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLLSIKLL 9
           SLL++KLL
Sbjct: 318 SLLALKLL 325



>XP_005277843
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLLSIKLL 9
           SLL++KLL
Sbjct: 318 SLLALKLL 325



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.144    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 432,226
Number of extensions: 597
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)