Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     390
NP_004648                                                              17    4312
NP_073752                                                              16    5631
NP_056051                                                              16    5631
XP_006720709                                                           16    5631
XP_005254660                                                           16    5631
XP_006714218                                                           16    5631
XP_006714217                                                           16    5631
XP_006714216                                                           16    5631
NP_689629                                                              16    7355
NP_001230175                                                           16    7355
NP_387504                                                              16    7355
NP_001013725                                                           16    7355
NP_919225                                                              16    7355
NP_001269665                                                           16    7355
NP_116201                                                              16    7355
NP_071751                                                              16    7355
NP_001139761                                                           16    7355
NP_055224                                                              16    7355
NP_001020418                                                           16    7355
XP_005254450                                                           16    7355
XP_005254449                                                           16    7355
XP_005251643                                                           16    7355
XP_006716939                                                           16    7355
XP_006716938                                                           16    7355
XP_006716937                                                           16    7355
XP_006716936                                                           16    7355
XP_005251642                                                           16    7355
XP_006716935                                                           16    7355
XP_006716934                                                           16    7355
XP_005250847                                                           16    7355
XP_005250846                                                           16    7355
XP_005273157                                                           16    7355
XP_005273158                                                           16    7355
XP_005273156                                                           16    7355
NP_063945                                                              15    9605
NP_001153648                                                           15    9605
NP_001153647                                                           15    9605
NP_001193583                                                           15    9605
NP_006303                                                              15    9605
NP_001070729                                                           15    9605
NP_620594                                                              15    9605
NP_062552                                                              15    9605
NP_060587                                                              15    9605
NP_006022                                                              15    9605
NP_065810                                                              15    9605
XP_005261187                                                           15    9605
XP_005261186                                                           15    9605
XP_005261185                                                           15    9605
XP_005261184                                                           15    9605
XP_005261183                                                           15    9605
XP_005261182                                                           15    9605
XP_005261181                                                           15    9605
XP_006720684                                                           15    9605
XP_006717250                                                           15    9605
XP_005266151                                                           15    9605
XP_006716156                                                           15    9605
XP_006716155                                                           15    9605
NP_699199                                                              15    9605
NP_075051                                                              15    9605
NP_689808                                                              15    9605
>NP_004295
          Length = 1620

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   PHNEAAIEI 9
           PHNEAA EI
Sbjct: 367 PHNEAAREI 375
>NP_004648
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PHNEAAIE 8
           PH+E A+E
Sbjct: 209 PHDEEALE 216
>NP_073752
          Length = 1460

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PHNEAAIEI 9
           P NE  IEI
Sbjct: 582 PSNEGVIEI 590
>NP_056051
          Length = 1469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    HNEAAIE 8
            HNE A+E
Sbjct: 1250 HNETALE 1256
>XP_006720709
          Length = 1189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PHNEAAIEI 9
           P NE  IEI
Sbjct: 311 PSNEGVIEI 319
>XP_005254660
          Length = 1460

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   PHNEAAIEI 9
           P NE  IEI
Sbjct: 582 PSNEGVIEI 590
>XP_006714218
          Length = 846

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HNEAAIE 8
           HNE A+E
Sbjct: 627 HNETALE 633
>XP_006714217
          Length = 1013

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HNEAAIE 8
           HNE A+E
Sbjct: 794 HNETALE 800
>XP_006714216
          Length = 1026

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HNEAAIE 8
           HNE A+E
Sbjct: 807 HNETALE 813
>NP_689629
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   PHNEAAIEI 9
           PH   AIE+
Sbjct: 130 PHQPVAIEL 138
>NP_001230175
          Length = 804

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PHNEAAIE 8
           PH EAA++
Sbjct: 226 PHLEAALQ 233
>NP_387504
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 772 PHSEAA 777
>NP_001013725
          Length = 1649

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HNEAAI 7
            HNEAA+
Sbjct: 1098 HNEAAL 1103
>NP_919225
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  PHNEAAIE 8
          P N+AA+E
Sbjct: 16 PENKAALE 23
>NP_001269665
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1  PHNEAAIEI 9
          PH E  +E+
Sbjct: 48 PHTEEEVEV 56
>NP_116201
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1  PHNEAAIEI 9
          PH E  +E+
Sbjct: 48 PHTEEEVEV 56
>NP_071751
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PHNEAAIE 8
           PH EAA++
Sbjct: 226 PHLEAALQ 233
>NP_001139761
          Length = 697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PHNEAAIE 8
           PH EAA++
Sbjct: 226 PHLEAALQ 233
>NP_055224
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAIEI 9
           NEAA+E+
Sbjct: 235 NEAALEV 241
>NP_001020418
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAIEI 9
           NEAA+E+
Sbjct: 235 NEAALEV 241
>XP_005254450
          Length = 1505

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HNEAAI 7
            HNEAA+
Sbjct: 1098 HNEAAL 1103
>XP_005254449
          Length = 1626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HNEAAI 7
            HNEAA+
Sbjct: 1098 HNEAAL 1103
>XP_005251643
          Length = 922

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 772 PHSEAA 777
>XP_006716939
          Length = 1060

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 772 PHSEAA 777
>XP_006716938
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 638 PHSEAA 643
>XP_006716937
          Length = 1155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 639 PHSEAA 644
>XP_006716936
          Length = 1194

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 678 PHSEAA 683
>XP_005251642
          Length = 1195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 679 PHSEAA 684
>XP_006716935
          Length = 1254

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 738 PHSEAA 743
>XP_006716934
          Length = 1287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   PHNEAA 6
           PH+EAA
Sbjct: 771 PHSEAA 776
>XP_005250847
          Length = 222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  PHNEAAIEI 9
          PH   AIE+
Sbjct: 19 PHQPVAIEL 27
>XP_005250846
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  PHNEAAIEI 9
          PH   AIE+
Sbjct: 38 PHQPVAIEL 46
>XP_005273157
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAIEI 9
           NEAA+E+
Sbjct: 143 NEAALEV 149
>XP_005273158
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAIEI 9
           NEAA+E+
Sbjct: 143 NEAALEV 149
>XP_005273156
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAIEI 9
           NEAA+E+
Sbjct: 235 NEAALEV 241
>NP_063945
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   PHNEAAI 7
           PH +AA+
Sbjct: 172 PHRDAAV 178
>NP_001153648
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HNEAAIE 8
          H EAA+E
Sbjct: 7  HKEAALE 13
>NP_001153647
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  HNEAAIE 8
          H EAA+E
Sbjct: 7  HKEAALE 13
>NP_001193583
          Length = 2504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PHNEAA 6
           PH EAA
Sbjct: 736 PHTEAA 741
>NP_006303
          Length = 2514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PHNEAA 6
           PH EAA
Sbjct: 754 PHTEAA 759
>NP_001070729
          Length = 2458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PHNEAA 6
           PH EAA
Sbjct: 736 PHTEAA 741
>NP_620594
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    PHNEAA 6
            PH EAA
Sbjct: 1140 PHEEAA 1145
>NP_062552
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  PHNEAA 6
          PH EAA
Sbjct: 34 PHQEAA 39
>NP_060587
          Length = 1224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  HNEAAIE 8
          HN+AA++
Sbjct: 22 HNKAAVD 28
>NP_006022
          Length = 3336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>NP_065810
          Length = 4700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAIEI 9
            H EA +EI
Sbjct: 4347 HEEAKVEI 4354
>XP_005261187
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>XP_005261186
          Length = 3139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 293 HHQAAIE 299
>XP_005261185
          Length = 3229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 293 HHQAAIE 299
>XP_005261184
          Length = 3258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>XP_005261183
          Length = 3268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>XP_005261182
          Length = 3346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>XP_005261181
          Length = 3347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HNEAAIE 8
           H++AAIE
Sbjct: 411 HHQAAIE 417
>XP_006720684
          Length = 4713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAIEI 9
            H EA +EI
Sbjct: 4360 HEEAKVEI 4367
>XP_006717250
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   PHNEAAI 7
           PH +AA+
Sbjct: 172 PHRDAAV 178
>XP_005266151
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   PHNEAAI 7
           PH +AA+
Sbjct: 172 PHRDAAV 178
>XP_006716156
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HNEAAIEI 9
           +NEAA+ I
Sbjct: 416 YNEAAVSI 423
>XP_006716155
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  HNEAAIEI 9
          +NEAA+ I
Sbjct: 89 YNEAAVSI 96
>NP_699199
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   PHNEAA 6
           PH EAA
Sbjct: 314 PHREAA 319
>NP_075051
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HNEAAIEI 9
           +NEAA+ I
Sbjct: 232 YNEAAVSI 239
>NP_689808
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   HNEAAIEI 9
           HN+  IE+
Sbjct: 400 HNDTVIEV 407
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.128    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 508,394
Number of extensions: 860
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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