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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     665
NP_001263689                                                           19     665
NP_001119584                                                           19     665
NP_001119586                                                           19     665
NP_001263625                                                           19     665
NP_001119590                                                           19     665
NP_001263690                                                           19     665
NP_001119585                                                           19     665
NP_001263624                                                           19     665
NP_001119588                                                           19     665
NP_001119587                                                           19     665
NP_001119589                                                           19     665
NP_001263627                                                           18    1935
NP_001263628                                                           18    1935
NP_001263626                                                           18    1935
NP_065759                                                              17    3301
NP_001128243                                                           17    3301
NP_001154824                                                           16    7355
NP_689534                                                              16    7355
XP_005253360                                                           16    7355
XP_005253359                                                           16    7355
XP_005269838                                                           16    7355
XP_005269835                                                           16    7355
NP_109587                                                              15    9605
NP_005379                                                              15    9605
NP_064506                                                              15    9605
NP_038464                                                              15    9605
NP_995314                                                              15    9605
XP_006712264                                                           15    9605
XP_006712263                                                           15    9605
XP_006712116                                                           15    9605

>NP_000537
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 159 AMAIYKQS 166



>NP_001263689
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 120 AMAIYKQS 127



>NP_001119584
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 159 AMAIYKQS 166



>NP_001119586
          Length = 341

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 159 AMAIYKQS 166



>NP_001263625
          Length = 302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 120 AMAIYKQS 127



>NP_001119590
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 120 AMAIYKQS 127



>NP_001263690
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 120 AMAIYKQS 127



>NP_001119585
          Length = 346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 159 AMAIYKQS 166



>NP_001263624
          Length = 307

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AMAIYKQS 9
           AMAIYKQS
Sbjct: 120 AMAIYKQS 127



>NP_001119588
          Length = 209

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  AMAIYKQS 9
          AMAIYKQS
Sbjct: 27 AMAIYKQS 34



>NP_001119587
          Length = 261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  AMAIYKQS 9
          AMAIYKQS
Sbjct: 27 AMAIYKQS 34



>NP_001119589
          Length = 214

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  AMAIYKQS 9
          AMAIYKQS
Sbjct: 27 AMAIYKQS 34



>NP_001263627
          Length = 182

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_001263628
          Length = 187

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_001263626
          Length = 234

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_065759
          Length = 1116

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PAMAI+K
Sbjct: 209 PAMAIFK 215



>NP_001128243
          Length = 1139

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PAMAI+K
Sbjct: 232 PAMAIFK 238



>NP_001154824
          Length = 1236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAMAIYKQ 8
           P M+IYK+
Sbjct: 698 PKMSIYKR 705



>NP_689534
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PA+AIY+
Sbjct: 220 PALAIYQ 226



>XP_005253360
          Length = 172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PA+AIY+
Sbjct: 119 PALAIYQ 125



>XP_005253359
          Length = 196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PA+AIY+
Sbjct: 143 PALAIYQ 149



>XP_005269838
          Length = 1236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAMAIYKQ 8
           P M+IYK+
Sbjct: 698 PKMSIYKR 705



>XP_005269835
          Length = 1239

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   PAMAIYKQ 8
           P M+IYK+
Sbjct: 702 PKMSIYKR 709



>NP_109587
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   PAMAIYK 7
           PAMAI++
Sbjct: 314 PAMAIFE 320



>NP_005379
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   PAMAIYK 7
           PA+ IYK
Sbjct: 234 PALLIYK 240



>NP_064506
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MAIYKQS 9
          +AIYKQ+
Sbjct: 74 LAIYKQT 80



>NP_038464
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1118 AVYKQS 1123



>NP_995314
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1124 AVYKQS 1129



>XP_006712264
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1116 AVYKQS 1121



>XP_006712263
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1122 AVYKQS 1127



>XP_006712116
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   PAMAIYKQS 9
           P MA+Y Q+
Sbjct: 274 PQMALYLQA 282



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.120    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 387,495
Number of extensions: 504
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)