Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              18    1482
NP_109593                                                              17    4312
NP_109595                                                              17    4312
NP_109596                                                              17    4312
NP_109592                                                              17    4312
NP_109594                                                              17    4312
NP_002839                                                              17    4312
NP_653244                                                              17    4312
XP_005262789                                                           17    4312
NP_001138498                                                           16    7355
NP_055856                                                              16    7355
NP_001242953                                                           16    7355
NP_067049                                                              16    7355
NP_001242955                                                           16    7355
NP_001242954                                                           16    7355
XP_006717997                                                           16    7355
XP_005270071                                                           16    7355
NP_078951                                                              15    9605
NP_000050                                                              15    9605
NP_055573                                                              15    9605
NP_001027467                                                           15    9605
NP_001273578                                                           15    9605
NP_003567                                                              15    9605
NP_055324                                                              15    9605
NP_113609                                                              15    9605
XP_006724285                                                           15    9605
XP_006722192                                                           15    9605
XP_005254135                                                           15    9605
XP_005254136                                                           15    9605
XP_005271603                                                           15    9605
XP_005271602                                                           15    9605
NP_055839                                                              15    9605

>NP_055365
          Length = 1032

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NVGLVSELR 9
           NV LVSELR
Sbjct: 276 NVDLVSELR 284



>NP_109593
          Length = 377

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VGLVSELR 9
           +GLVSE+R
Sbjct: 326 LGLVSEMR 333



>NP_109595
          Length = 377

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VGLVSELR 9
           +GLVSE+R
Sbjct: 326 LGLVSEMR 333



>NP_109596
          Length = 405

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VGLVSELR 9
           +GLVSE+R
Sbjct: 354 LGLVSEMR 361



>NP_109592
          Length = 1216

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VGLVSELR 9
            +GLVSE+R
Sbjct: 1165 LGLVSEMR 1172



>NP_109594
          Length = 405

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VGLVSELR 9
           +GLVSE+R
Sbjct: 354 LGLVSEMR 361



>NP_002839
          Length = 1188

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    VGLVSELR 9
            +GLVSE+R
Sbjct: 1137 LGLVSEMR 1144



>NP_653244
          Length = 688

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N GLVSE R
Sbjct: 505 NCGLVSEQR 513



>XP_005262789
          Length = 352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N GLVSE R
Sbjct: 169 NCGLVSEQR 177



>NP_001138498
          Length = 2299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    GLVSELR 9
            GLV+ELR
Sbjct: 2230 GLVAELR 2236



>NP_055856
          Length = 413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NVGLVSEL 8
          N GLVSE+
Sbjct: 31 NFGLVSEV 38



>NP_001242953
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 617 GLVTELR 623



>NP_067049
          Length = 698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 601 GLVTELR 607



>NP_001242955
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 511 GLVTELR 517



>NP_001242954
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 607 GLVTELR 613



>XP_006717997
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 511 GLVTELR 517



>XP_005270071
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GLVSELR 9
           GLV+ELR
Sbjct: 527 GLVTELR 533



>NP_078951
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NVGLVSELR 9
           N+ LVSEL+
Sbjct: 854 NLQLVSELK 862



>NP_000050
          Length = 3418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N GL+S L+
Sbjct: 576 NAGLISTLK 584



>NP_055573
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GLVSELR 9
           GLV ELR
Sbjct: 137 GLVQELR 143



>NP_001027467
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N+G + ELR
Sbjct: 385 NLGSIEELR 393



>NP_001273578
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N+G + ELR
Sbjct: 366 NLGSIEELR 374



>NP_003567
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N+G + ELR
Sbjct: 397 NLGSIEELR 405



>NP_055324
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  GLVSELR 9
          GL+SEL+
Sbjct: 8  GLISELK 14



>NP_113609
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N G++ ELR
Sbjct: 487 NKGIIRELR 495



>XP_006724285
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  GLVSELR 9
          GL+SEL+
Sbjct: 8  GLISELK 14



>XP_006722192
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N G++ ELR
Sbjct: 324 NKGIIRELR 332



>XP_005254135
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N+G + ELR
Sbjct: 287 NLGSIEELR 295



>XP_005254136
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NVGLVSELR 9
           N+G + ELR
Sbjct: 287 NLGSIEELR 295



>XP_005271603
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GLVSELR 9
           GLV ELR
Sbjct: 137 GLVQELR 143



>XP_005271602
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GLVSELR 9
           GLV ELR
Sbjct: 137 GLVQELR 143



>NP_055839
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VGLVSELR 9
           VGLV +LR
Sbjct: 720 VGLVRDLR 727



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.138    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,395
Number of extensions: 612
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)