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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_109590                                                              18    1135
NP_001008844                                                           16    7355
NP_004406                                                              16    7355
NP_001189368                                                           16    7355
NP_775321                                                              16    7355
NP_060132                                                              16    7355
NP_001106649                                                           16    7355
NP_619728                                                              16    7355
NP_006590                                                              16    7355
NP_001164009                                                           16    7355
NP_619727                                                              16    7355
NP_775322                                                              16    7355
NP_001170781                                                           16    7355
NP_775814                                                              16    7355
XP_005255834                                                           16    7355
XP_006721188                                                           16    7355
XP_006717022                                                           16    7355
XP_006713590                                                           16    7355
XP_006713589                                                           16    7355
XP_005247255                                                           16    7355
NP_001170782                                                           16    7355
NP_003042                                                              15    9605
NP_001159968                                                           15    9605
NP_055845                                                              15    9605
XP_005248148                                                           15    9605
XP_005248147                                                           15    9605
XP_005248146                                                           15    9605
XP_005248145                                                           15    9605
XP_005248144                                                           15    9605
XP_005248143                                                           15    9605
XP_005248142                                                           15    9605
XP_005248141                                                           15    9605
XP_005248140                                                           15    9605
XP_005248139                                                           15    9605

>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           NTIQQLMM
Sbjct: 380 NTIQQLMM 387



>NP_109590
          Length = 1906

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NTIQQLMMS 9
           NT+QQL++S
Sbjct: 447 NTVQQLLLS 455



>NP_001008844
          Length = 2272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NTIQQLMMS 9
          NTIQ+L+ +
Sbjct: 69 NTIQELLQN 77



>NP_004406
          Length = 2871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NTIQQLMMS 9
          NTIQ+L+ +
Sbjct: 69 NTIQELLQN 77



>NP_001189368
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 346 NTIQQL 351



>NP_775321
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 697 NTIQQL 702



>NP_060132
          Length = 2022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    NTIQQLMMS 9
            NT+++LM++
Sbjct: 1898 NTLEELMLA 1906



>NP_001106649
          Length = 1548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 790 NTIQQL 795



>NP_619728
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 697 NTIQQL 702



>NP_006590
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 773 NTIQQL 778



>NP_001164009
          Length = 539

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           +T+QQL+M
Sbjct: 151 STLQQLLM 158



>NP_619727
          Length = 1549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 791 NTIQQL 796



>NP_775322
          Length = 1455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 697 NTIQQL 702



>NP_001170781
          Length = 2017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    NTIQQLMMS 9
            NT+++LM++
Sbjct: 1893 NTLEELMLA 1901



>NP_775814
          Length = 767

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           +T+QQL+M
Sbjct: 151 STLQQLLM 158



>XP_005255834
          Length = 1264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 506 NTIQQL 511



>XP_006721188
          Length = 1484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NTIQQL
Sbjct: 726 NTIQQL 731



>XP_006717022
          Length = 1054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   NTIQQLMMS 9
           NT+++LM++
Sbjct: 930 NTLEELMLA 938



>XP_006713590
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           +T+QQL+M
Sbjct: 151 STLQQLLM 158



>XP_006713589
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           +T+QQL+M
Sbjct: 151 STLQQLLM 158



>XP_005247255
          Length = 795

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   NTIQQLMM 8
           +T+QQL+M
Sbjct: 179 STLQQLLM 186



>NP_001170782
          Length = 2017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    NTIQQLMMS 9
            NT+++LM++
Sbjct: 1893 NTLEELMLA 1901



>NP_003042
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NT+QQL
Sbjct: 107 NTVQQL 112



>NP_001159968
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NTIQQL 6
           NT+QQL
Sbjct: 107 NTVQQL 112



>NP_055845
          Length = 3013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 598 NTLQALML 605



>XP_005248148
          Length = 3028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 613 NTLQALML 620



>XP_005248147
          Length = 3028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 613 NTLQALML 620



>XP_005248146
          Length = 3073

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 705 NTLQALML 712



>XP_005248145
          Length = 3079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 664 NTLQALML 671



>XP_005248144
          Length = 3099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 690 NTLQALML 697



>XP_005248143
          Length = 3105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 690 NTLQALML 697



>XP_005248142
          Length = 3114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 705 NTLQALML 712



>XP_005248141
          Length = 3119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 705 NTLQALML 712



>XP_005248140
          Length = 3119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 705 NTLQALML 712



>XP_005248139
          Length = 3120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NTIQQLMM 8
           NT+Q LM+
Sbjct: 705 NTLQALML 712



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.118    0.281 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,569
Number of extensions: 282
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)