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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     299
NP_001004754                                                           16    5631
NP_891549                                                              16    5631
NP_001070650                                                           16    7355
NP_036550                                                              16    7355
NP_005550                                                              16    7355
XP_005257253                                                           16    7355
XP_005257252                                                           16    7355
XP_006718995                                                           16    7355
XP_005274425                                                           16    7355
XP_006718994                                                           16    7355
XP_006718993                                                           16    7355
XP_005274424                                                           16    7355
XP_005274423                                                           16    7355
NP_203753                                                              16    7355
NP_001186135                                                           15    9605
NP_001123380                                                           15    9605
NP_006801                                                              15    9605
XP_006726157                                                           15    9605
XP_005275612                                                           15    9605
XP_005275610                                                           15    9605
XP_006726064                                                           15    9605
XP_005275460                                                           15    9605
XP_006715157                                                           15    9605
XP_005249149                                                           15    9605
NP_002254                                                              15    9605

>NP_004251
          Length = 1208

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NRAVARIFH 9
            +RAVARIFH
Sbjct: 1157 SRAVARIFH 1165



>NP_001004754
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RAVARIFH 9
           RA+ R+FH
Sbjct: 301 RAIFRMFH 308



>NP_891549
          Length = 712

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AVARIFH 9
           AVAR++H
Sbjct: 95  AVARLYH 101



>NP_001070650
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NRAVARIFH 9
           NR+V  +FH
Sbjct: 429 NRSVYDVFH 437



>NP_036550
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NRAVARIFH 9
           NR+V  +FH
Sbjct: 405 NRSVYDVFH 413



>NP_005550
          Length = 3075

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    NRAVARIF 8
            NRA  RIF
Sbjct: 1644 NRATERIF 1651



>XP_005257253
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NRAVARIFH 9
           NR+V  +FH
Sbjct: 405 NRSVYDVFH 413



>XP_005257252
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NRAVARIFH 9
           NR+V  +FH
Sbjct: 429 NRSVYDVFH 437



>XP_006718995
          Length = 2382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2361 NRARAKEFH 2369



>XP_005274425
          Length = 2566

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2545 NRARAKEFH 2553



>XP_006718994
          Length = 2567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2546 NRARAKEFH 2554



>XP_006718993
          Length = 2602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2581 NRARAKEFH 2589



>XP_005274424
          Length = 2614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2593 NRARAKEFH 2601



>XP_005274423
          Length = 2615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2594 NRARAKEFH 2602



>NP_203753
          Length = 2601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    NRAVARIFH 9
            NRA A+ FH
Sbjct: 2580 NRARAKEFH 2588



>NP_001186135
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NRAVARIF 8
           N AVAR+F
Sbjct: 219 NVAVARLF 226



>NP_001123380
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AVARIFH 9
           AVAR FH
Sbjct: 346 AVARRFH 352



>NP_006801
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NRAVARIFH 9
           N+A+A  FH
Sbjct: 340 NKALAERFH 348



>XP_006726157
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 263 NRAVAR 268



>XP_005275612
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 482 NRAVAR 487



>XP_005275610
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 523 NRAVAR 528



>XP_006726064
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 263 NRAVAR 268



>XP_005275460
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 523 NRAVAR 528



>XP_006715157
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 263 NRAVAR 268



>XP_005249149
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 523 NRAVAR 528



>NP_002254
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NRAVAR 6
           NRAVAR
Sbjct: 523 NRAVAR 528



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.142    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 270,871
Number of extensions: 321
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)