Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              21     175
XP_005251512                                                           21     175
NP_733468                                                              17    2528
NP_653091                                                              17    2528
NP_002494                                                              17    3301
NP_001230045                                                           17    3301
XP_006723290                                                           17    3301
XP_006723289                                                           17    3301
NP_060009                                                              17    4312
XP_006721125                                                           17    4312
NP_446464                                                              16    5631
NP_003733                                                              16    5631
NP_000041                                                              16    5631
NP_001185731                                                           16    5631
NP_001185730                                                           16    5631
NP_004682                                                              16    5631
NP_003751                                                              16    5631
NP_001001732                                                           16    5631
NP_001185732                                                           16    5631
XP_006717897                                                           16    5631
XP_006717896                                                           16    5631
XP_005272257                                                           16    5631
XP_005272256                                                           16    5631
XP_006712881                                                           16    5631
XP_006712880                                                           16    5631
NP_001153219                                                           16    5631
NP_619642                                                              16    7355
NP_803884                                                              16    7355
NP_001123508                                                           16    7355
NP_001123507                                                           16    7355
NP_001123505                                                           16    7355
NP_115495                                                              15    9605
NP_002968                                                              15    9605
NP_733820                                                              15    9605
NP_055854                                                              15    9605
NP_113584                                                              15    9605
NP_001135993                                                           15    9605
NP_001135991                                                           15    9605
NP_071357                                                              15    9605
NP_942144                                                              15    9605
NP_001135992                                                           15    9605
NP_937886                                                              15    9605
NP_056240                                                              15    9605
NP_001171671                                                           15    9605
NP_078881                                                              15    9605
NP_003282                                                              15    9605
NP_003626                                                              15    9605
NP_006768                                                              15    9605
NP_001036038                                                           15    9605
XP_006725470                                                           15    9605
XP_005262029                                                           15    9605
XP_005262028                                                           15    9605
XP_005262026                                                           15    9605
XP_006724637                                                           15    9605
XP_005262025                                                           15    9605
XP_005262024                                                           15    9605
XP_005262023                                                           15    9605
XP_005262022                                                           15    9605
XP_006724304                                                           15    9605
XP_006722082                                                           15    9605
XP_006722079                                                           15    9605
XP_006722078                                                           15    9605
XP_005254130                                                           15    9605
XP_005254129                                                           15    9605
XP_005254128                                                           15    9605
XP_005254127                                                           15    9605
XP_005270313                                                           15    9605
XP_005270312                                                           15    9605
XP_006718109                                                           15    9605
XP_005263273                                                           15    9605
XP_005263272                                                           15    9605
XP_005263271                                                           15    9605
XP_005246814                                                           15    9605
NP_078984                                                              15    9605
NP_056507                                                              15    9605
NP_061753                                                              15    9605
>NP_004963
          Length = 1132

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQTPLAIYN 9
           +QTPLAIYN
Sbjct: 194 DQTPLAIYN 202
>XP_005251512
          Length = 1132

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NQTPLAIYN 9
           +QTPLAIYN
Sbjct: 194 DQTPLAIYN 202
>NP_733468
          Length = 2342

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           +Q PLA YN
Sbjct: 610 SQVPLAAYN 618
>NP_653091
          Length = 2316

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           +Q PLA YN
Sbjct: 584 SQVPLAAYN 592
>NP_002494
          Length = 356

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A+Y
Sbjct: 173 NHTPVALY 180
>NP_001230045
          Length = 270

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  NQTPLAIY 8
          N TP+A+Y
Sbjct: 87 NHTPVALY 94
>XP_006723290
          Length = 351

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A+Y
Sbjct: 168 NHTPVALY 175
>XP_006723289
          Length = 416

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A+Y
Sbjct: 233 NHTPVALY 240
>NP_060009
          Length = 4116

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TPLAIYN 9
            TPL++YN
Sbjct: 2544 TPLSMYN 2550
>XP_006721125
          Length = 4070

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    TPLAIYN 9
            TPL++YN
Sbjct: 2498 TPLSMYN 2504
>NP_446464
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QTPLAIYN 9
           ++PL++YN
Sbjct: 364 ESPLSLYN 371
>NP_003733
          Length = 1321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    QTPLAIYN 9
            Q P+++YN
Sbjct: 1058 QPPISVYN 1065
>NP_000041
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QTPLAIYN 9
           ++PL++YN
Sbjct: 364 ESPLSLYN 371
>NP_001185731
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          QTP A+Y
Sbjct: 43 QTPTAVY 49
>NP_001185730
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          QTP A+Y
Sbjct: 36 QTPTAVY 42
>NP_004682
          Length = 351

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           QTP  +YN
Sbjct: 145 QTPAELYN 152
>NP_003751
          Length = 1585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          QTP A+Y
Sbjct: 36 QTPTAVY 42
>NP_001001732
          Length = 322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A++
Sbjct: 227 NNTPMAVF 234
>NP_001185732
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          QTP A+Y
Sbjct: 47 QTPTAVY 53
>XP_006717897
          Length = 1160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A++
Sbjct: 986 NNTPMAVF 993
>XP_006717896
          Length = 1186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            N TP+A++
Sbjct: 1012 NNTPMAVF 1019
>XP_005272257
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QTPLAIYN 9
           ++PL++YN
Sbjct: 364 ESPLSLYN 371
>XP_005272256
          Length = 444

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QTPLAIYN 9
           ++PL++YN
Sbjct: 396 ESPLSLYN 403
>XP_006712881
          Length = 1335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    QTPLAIYN 9
            Q P+++YN
Sbjct: 1072 QPPISVYN 1079
>XP_006712880
          Length = 1335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    QTPLAIYN 9
            Q P+++YN
Sbjct: 1072 QPPISVYN 1079
>NP_001153219
          Length = 401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   NQTPLAIY 8
           N TP+A++
Sbjct: 227 NNTPMAVF 234
>NP_619642
          Length = 841

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           N+ PL+ YN
Sbjct: 442 NRDPLSSYN 450
>NP_803884
          Length = 587

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           N+ PL+ YN
Sbjct: 188 NRDPLSSYN 196
>NP_001123508
          Length = 189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           N TP+ +Y+
Sbjct: 108 NSTPVRVYS 116
>NP_001123507
          Length = 189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           N TP+ +Y+
Sbjct: 108 NSTPVRVYS 116
>NP_001123505
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   NQTPLAIYN 9
           N TP+ +Y+
Sbjct: 127 NSTPVRVYS 135
>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    NQTPLAIYN 9
            NQ PL +++
Sbjct: 3193 NQAPLGLFS 3201
>NP_002968
          Length = 1977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           NQ+PL+I
Sbjct: 533 NQSPLSI 539
>NP_733820
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NQTPLAIY 8
           N+TP  IY
Sbjct: 126 NETPQPIY 133
>NP_055854
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   NQTPLAIY 8
           N+TP  IY
Sbjct: 131 NETPQPIY 138
>NP_113584
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>NP_001135993
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           Q P+ +YN
Sbjct: 451 QVPMELYN 458
>NP_001135991
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           Q P+ +YN
Sbjct: 451 QVPMELYN 458
>NP_071357
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           Q P+ +YN
Sbjct: 451 QVPMELYN 458
>NP_942144
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           Q P+ +YN
Sbjct: 451 QVPMELYN 458
>NP_001135992
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           Q P+ +YN
Sbjct: 451 QVPMELYN 458
>NP_937886
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QTPLAI 7
           QTPLA+
Sbjct: 248 QTPLAV 253
>NP_056240
          Length = 1720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  QTPLAIYN 9
          + PL +YN
Sbjct: 82 ELPLTVYN 89
>NP_001171671
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NQTPLA 6
           NQTPL+
Sbjct: 284 NQTPLS 289
>NP_078881
          Length = 786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PLAIYN 9
           P+A+YN
Sbjct: 610 PVAVYN 615
>NP_003282
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            +QT LA+Y
Sbjct: 1088 DQTALAVY 1095
>NP_003626
          Length = 883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           +QTPLA+
Sbjct: 737 SQTPLAL 743
>NP_006768
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   NQTPLA 6
           NQTPL+
Sbjct: 284 NQTPLS 289
>NP_001036038
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           QTP A Y+
Sbjct: 147 QTPYAFYH 154
>XP_006725470
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          Q PLA+Y
Sbjct: 86 QQPLALY 92
>XP_005262029
          Length = 4240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_005262028
          Length = 4341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_005262026
          Length = 4359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_006724637
          Length = 4365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1711 NDTPLAL 1717
>XP_005262025
          Length = 4373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_005262024
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_005262023
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_005262022
          Length = 4374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NQTPLAI 7
            N TPLA+
Sbjct: 1720 NDTPLAL 1726
>XP_006724304
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QTPLAIY 8
          Q PLA+Y
Sbjct: 86 QQPLALY 92
>XP_006722082
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QTPLAIYN 9
           QTP A Y+
Sbjct: 147 QTPYAFYH 154
>XP_006722079
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QTPLAIYN 9
          QTP A Y+
Sbjct: 17 QTPYAFYH 24
>XP_006722078
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  QTPLAIYN 9
          QTP A Y+
Sbjct: 17 QTPYAFYH 24
>XP_005254130
          Length = 1162

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            +QT LA+Y
Sbjct: 1101 DQTALAVY 1108
>XP_005254129
          Length = 1262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            +QT LA+Y
Sbjct: 1101 DQTALAVY 1108
>XP_005254128
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            +QT LA+Y
Sbjct: 1088 DQTALAVY 1095
>XP_005254127
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NQTPLAIY 8
            +QT LA+Y
Sbjct: 1101 DQTALAVY 1108
>XP_005270313
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           +QTPLA+
Sbjct: 363 SQTPLAL 369
>XP_005270312
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           +QTPLA+
Sbjct: 737 SQTPLAL 743
>XP_006718109
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           +QTPLA+
Sbjct: 675 SQTPLAL 681
>XP_005263273
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PLAIYN 9
           P+A+YN
Sbjct: 518 PVAVYN 523
>XP_005263272
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PLAIYN 9
           P+A+YN
Sbjct: 610 PVAVYN 615
>XP_005263271
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PLAIYN 9
           P+A+YN
Sbjct: 586 PVAVYN 591
>XP_005246814
          Length = 1988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NQTPLAI 7
           NQ+PL+I
Sbjct: 533 NQSPLSI 539
>NP_078984
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QTPLAI 7
           QTPLA+
Sbjct: 248 QTPLAV 253
>NP_056507
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PLAIYN 9
           PL++YN
Sbjct: 871 PLSVYN 876
>NP_061753
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   QTPLAIY 8
           +TPLA++
Sbjct: 362 ETPLAVF 368
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.129    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 724,247
Number of extensions: 1169
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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