Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           19     665
NP_001020014                                                           19     665
NP_055903                                                              19     665
NP_001269516                                                           17    2528
NP_004276                                                              17    2528
XP_006711115                                                           17    2528
XP_005263598                                                           17    2528
XP_005263597                                                           17    2528
XP_005263596                                                           17    2528
NP_001269621                                                           16    5631
NP_996879                                                              16    7355
NP_001363                                                              16    7355
NP_079420                                                              16    7355
NP_001095876                                                           16    7355
XP_006721530                                                           16    7355
XP_006721529                                                           16    7355
XP_006721528                                                           16    7355
XP_006714389                                                           16    7355
XP_005263303                                                           16    7355
XP_005263302                                                           16    7355
XP_006710493                                                           16    7355
XP_006710490                                                           16    7355
XP_006710489                                                           16    7355
>NP_001155973
          Length = 1833

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  NNAKRVAV 8
          NNAKRVAV
Sbjct: 68 NNAKRVAV 75
>NP_001020014
          Length = 1029

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NNAKRVAV 8
           NNAKRVAV
Sbjct: 104 NNAKRVAV 111
>NP_055903
          Length = 1723

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  NNAKRVAV 8
          NNAKRVAV
Sbjct: 68 NNAKRVAV 75
>NP_001269516
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 748 NRAKKVAVL 756
>NP_004276
          Length = 791

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 737 NRAKKVAVL 745
>XP_006711115
          Length = 791

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 737 NRAKKVAVL 745
>XP_005263598
          Length = 798

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 744 NRAKKVAVL 752
>XP_005263597
          Length = 800

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 746 NRAKKVAVL 754
>XP_005263596
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   NNAKRVAVL 9
           N AK+VAVL
Sbjct: 748 NRAKKVAVL 756
>NP_001269621
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NNAKRVAVL 9
          N AKRVA++
Sbjct: 4  NMAKRVAIV 12
>NP_996879
          Length = 2223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NNAKRVAVL 9
           NN KR+A +
Sbjct: 570 NNTKRLATI 578
>NP_001363
          Length = 4486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NNAKRVAV 8
            NN K+VA+
Sbjct: 1199 NNIKKVAI 1206
>NP_079420
          Length = 1244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NNAKRVAVL 9
          NN K VAVL
Sbjct: 2  NNQKVVAVL 10
>NP_001095876
          Length = 1244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NNAKRVAVL 9
          NN K VAVL
Sbjct: 2  NNQKVVAVL 10
>XP_006721530
          Length = 2746

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NNAKRVAV 8
            NN K+VA+
Sbjct: 1199 NNIKKVAI 1206
>XP_006721529
          Length = 2817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NNAKRVAV 8
            NN K+VA+
Sbjct: 1199 NNIKKVAI 1206
>XP_006721528
          Length = 3869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    NNAKRVAV 8
            NN K+VA+
Sbjct: 1199 NNIKKVAI 1206
>XP_006714389
          Length = 1225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NNAKRVAVL 9
          NN K VAVL
Sbjct: 2  NNQKVVAVL 10
>XP_005263303
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NNAKRVAVL 9
          NN K VAVL
Sbjct: 2  NNQKVVAVL 10
>XP_005263302
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NNAKRVAVL 9
          NN K VAVL
Sbjct: 2  NNQKVVAVL 10
>XP_006710493
          Length = 1305

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NNAKRVAVL 9
           NN KR+A +
Sbjct: 570 NNTKRLATI 578
>XP_006710490
          Length = 2158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NNAKRVAVL 9
           NN KR+A +
Sbjct: 505 NNTKRLATI 513
>XP_006710489
          Length = 2185

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NNAKRVAVL 9
           NN KR+A +
Sbjct: 570 NNTKRLATI 578
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.124    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 308,089
Number of extensions: 407
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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