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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185761                                                           19     665
NP_055459                                                              19     665
XP_006726410                                                           19     665
XP_006711719                                                           19     665
XP_006711716                                                           19     665
XP_005273038                                                           19     665
XP_006711164                                                           19     665
NP_078951                                                              17    3301
NP_001136018                                                           16    7355
NP_361013                                                              16    7355
NP_056503                                                              15    9605
NP_079199                                                              15    9605

>NP_001185761
          Length = 2240

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2225 NLEVKSLRA 2233



>NP_055459
          Length = 2346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2331 NLEVKSLRA 2339



>XP_006726410
          Length = 2087

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2072 NLEVKSLRA 2080



>XP_006711719
          Length = 2302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2287 NLEVKSLRA 2295



>XP_006711716
          Length = 2411

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2396 NLEVKSLRA 2404



>XP_005273038
          Length = 2509

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2494 NLEVKSLRA 2502



>XP_006711164
          Length = 2087

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NLEVKSVRA 9
            NLEVKS+RA
Sbjct: 2072 NLEVKSLRA 2080



>NP_078951
          Length = 1186

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NLEVKSVRA 9
           NLE++ +RA
Sbjct: 938 NLEIREIRA 946



>NP_001136018
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NLEVKSVRA 9
           N  VKSVRA
Sbjct: 715 NESVKSVRA 723



>NP_361013
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   NLEVKSVRA 9
           N  VKSVRA
Sbjct: 715 NESVKSVRA 723



>NP_056503
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEVKSVRA 9
           +E+KSVR+
Sbjct: 441 VEIKSVRS 448



>NP_079199
          Length = 1887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    NLEVKS 6
            NLEVKS
Sbjct: 1720 NLEVKS 1725



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.121    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 245,771
Number of extensions: 226
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)