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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           20     299
NP_060785                                                              20     299
NP_361014                                                              20     299
NP_001240658                                                           16    7355
NP_001240657                                                           16    7355
NP_001240656                                                           16    7355
NP_001240659                                                           16    7355
NP_001240662                                                           16    7355
NP_001240661                                                           16    7355
NP_067647                                                              16    7355
NP_036265                                                              16    7355
NP_001276962                                                           16    7355
NP_057212                                                              16    7355
NP_542401                                                              16    7355
NP_001243089                                                           15    9605
NP_079273                                                              15    9605
NP_055314                                                              15    9605
NP_722576                                                              15    9605
NP_938147                                                              15    9605
NP_077006                                                              15    9605
XP_005262424                                                           15    9605
XP_005262182                                                           15    9605
XP_005262181                                                           15    9605
XP_005261392                                                           15    9605
XP_006721017                                                           15    9605
XP_006721016                                                           15    9605
XP_006712802                                                           15    9605
XP_006712801                                                           15    9605
XP_006712800                                                           15    9605
NP_689639                                                              15    9605

>NP_001013433
          Length = 589

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NKHQSAVT 8
           NKHQSAVT
Sbjct: 503 NKHQSAVT 510



>NP_060785
          Length = 627

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NKHQSAVT 8
           NKHQSAVT
Sbjct: 541 NKHQSAVT 548



>NP_361014
          Length = 707

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   NKHQSAVT 8
           NKHQSAVT
Sbjct: 621 NKHQSAVT 628



>NP_001240658
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 559 HQSAIT 564



>NP_001240657
          Length = 724

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 574 HQSAIT 579



>NP_001240656
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 634 HQSAIT 639



>NP_001240659
          Length = 626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 476 HQSAIT 481



>NP_001240662
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 451 HQSAIT 456



>NP_001240661
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 475 HQSAIT 480



>NP_067647
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HQSAVT 8
           HQSA+T
Sbjct: 607 HQSAIT 612



>NP_036265
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KHQSAVT 8
           KH SAVT
Sbjct: 317 KHPSAVT 323



>NP_001276962
          Length = 726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KHQSAVT 8
           KH SAVT
Sbjct: 317 KHPSAVT 323



>NP_057212
          Length = 874

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KHQSAVT 8
           KH SAVT
Sbjct: 317 KHPSAVT 323



>NP_542401
          Length = 324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KHQSAVTR 9
           KH S++TR
Sbjct: 272 KHGSSITR 279



>NP_001243089
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++HQ+ VTR
Sbjct: 145 SEHQTNVTR 153



>NP_079273
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++HQ+ VTR
Sbjct: 145 SEHQTNVTR 153



>NP_055314
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++H S+VTR
Sbjct: 317 SRHGSSVTR 325



>NP_722576
          Length = 3080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KHQSAVTR 9
           K QSAVT+
Sbjct: 392 KSQSAVTK 399



>NP_938147
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++H S+VTR
Sbjct: 317 SRHGSSVTR 325



>NP_077006
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NKHQSAV 7
           NKH SAV
Sbjct: 345 NKHISAV 351



>XP_005262424
          Length = 2875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KHQSAVTR 9
           K QSAVT+
Sbjct: 187 KSQSAVTK 194



>XP_005262182
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++H S+VTR
Sbjct: 317 SRHGSSVTR 325



>XP_005262181
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++H S+VTR
Sbjct: 334 SRHGSSVTR 342



>XP_005261392
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KHQSAVTR 9
          KH SAV R
Sbjct: 43 KHHSAVFR 50



>XP_006721017
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++HQ+ VTR
Sbjct: 145 SEHQTNVTR 153



>XP_006721016
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NKHQSAVTR 9
           ++HQ+ VTR
Sbjct: 145 SEHQTNVTR 153



>XP_006712802
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NKHQSAV 7
           NKH SAV
Sbjct: 345 NKHISAV 351



>XP_006712801
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NKHQSAV 7
           NKH SAV
Sbjct: 345 NKHISAV 351



>XP_006712800
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   NKHQSAV 7
           NKH SAV
Sbjct: 305 NKHISAV 311



>NP_689639
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KHQSAVTR 9
           KH SAV R
Sbjct: 239 KHHSAVFR 246



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.108    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,637
Number of extensions: 401
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)