Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005248121                                                           17    2528
NP_003961                                                              17    3301
XP_006716300                                                           17    3301
NP_001135741                                                           17    4312
NP_000812                                                              17    4312
NP_060347                                                              17    4312
XP_005264316                                                           17    4312
NP_055877                                                              16    5631
NP_001275656                                                           16    5631
NP_849157                                                              16    5631
NP_055621                                                              16    5631
NP_001129471                                                           16    5631
NP_003387                                                              16    5631
XP_006725694                                                           16    5631
XP_005257694                                                           16    5631
XP_006717794                                                           16    5631
XP_005269715                                                           16    5631
XP_006717793                                                           16    5631
XP_005269713                                                           16    5631
XP_006714159                                                           16    5631
XP_006714158                                                           16    5631
XP_006714157                                                           16    5631
NP_000029                                                              16    7355
NP_001120983                                                           16    7355
NP_001120982                                                           16    7355
NP_001040625                                                           16    7355
NP_005854                                                              16    7355
NP_001138923                                                           16    7355
NP_004481                                                              15    9605
NP_060145                                                              15    9605
NP_001165447                                                           15    9605
NP_059129                                                              15    9605
NP_001093155                                                           15    9605
XP_006716845                                                           15    9605
XP_005251541                                                           15    9605
XP_006716843                                                           15    9605
XP_005251540                                                           15    9605
XP_006714950                                                           15    9605
XP_006712520                                                           15    9605
XP_005246534                                                           15    9605
NP_115887                                                              15    9605
NP_066553                                                              15    9605
>XP_005248121
          Length = 1543

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  NISPFRSVG 9
          NISPF  VG
Sbjct: 15 NISPFPFVG 23
>NP_003961
          Length = 1465

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   PFRSVG 9
           PFRSVG
Sbjct: 255 PFRSVG 260
>XP_006716300
          Length = 1445

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   PFRSVG 9
           PFRSVG
Sbjct: 255 PFRSVG 260
>NP_001135741
          Length = 701

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ PF +VG
Sbjct: 667 NLRPFEAVG 675
>NP_000812
          Length = 758

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ PF +VG
Sbjct: 724 NLRPFEAVG 732
>NP_060347
          Length = 371

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ P R+VG
Sbjct: 205 NVHPLRAVG 213
>XP_005264316
          Length = 756

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ PF +VG
Sbjct: 722 NLRPFEAVG 730
>NP_055877
          Length = 1664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>NP_001275656
          Length = 1400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>NP_849157
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           +ISP R+VG
Sbjct: 687 SISPSRNVG 695
>NP_055621
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SPFRSVG 9
           SP RSVG
Sbjct: 781 SPIRSVG 787
>NP_001129471
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SPFRSVG 9
           SP RSVG
Sbjct: 781 SPIRSVG 787
>NP_003387
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISPFRSVG 9
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>XP_006725694
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISPFRSVG 9
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>XP_005257694
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISPFRSVG 9
           +SPFR  G
Sbjct: 228 LSPFRETG 235
>XP_006717794
          Length = 1194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>XP_005269715
          Length = 1230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>XP_006717793
          Length = 1603

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>XP_005269713
          Length = 1646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           N+SPF S+
Sbjct: 142 NLSPFDSI 149
>XP_006714159
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           +ISP R+VG
Sbjct: 687 SISPSRNVG 695
>XP_006714158
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           +ISP R+VG
Sbjct: 687 SISPSRNVG 695
>XP_006714157
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           +ISP R+VG
Sbjct: 687 SISPSRNVG 695
>NP_000029
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISPFRSVG 9
            +SP+RS G
Sbjct: 1099 VSPYRSRG 1106
>NP_001120983
          Length = 2825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISPFRSVG 9
            +SP+RS G
Sbjct: 1081 VSPYRSRG 1088
>NP_001120982
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    ISPFRSVG 9
            +SP+RS G
Sbjct: 1099 VSPYRSRG 1106
>NP_001040625
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISPFRSVG 9
           I+PF+S G
Sbjct: 500 IAPFQSAG 507
>NP_005854
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISPFRSVG 9
           I+PF+S G
Sbjct: 446 IAPFQSAG 453
>NP_001138923
          Length = 870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SPFRSV 8
           SPFRS+
Sbjct: 140 SPFRSI 145
>NP_004481
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           +ISP RS+
Sbjct: 364 SISPMRSI 371
>NP_060145
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           ++SP R+VG
Sbjct: 417 SVSPERAVG 425
>NP_001165447
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           ++SP R+VG
Sbjct: 417 SVSPERAVG 425
>NP_059129
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ISPFRSVG 9
           ++PF+S+G
Sbjct: 378 LNPFQSLG 385
>NP_001093155
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ PF +VG
Sbjct: 200 NMLPFMAVG 208
>XP_006716845
          Length = 546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ISPFRS 7
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_005251541
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ISPFRS 7
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_006716843
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ISPFRS 7
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_005251540
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ISPFRS 7
           +SPFRS
Sbjct: 185 LSPFRS 190
>XP_006714950
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   NISPFRSVG 9
           ++SP R+VG
Sbjct: 417 SVSPERAVG 425
>XP_006712520
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           +ISP RS+
Sbjct: 164 SISPMRSI 171
>XP_005246534
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   NISPFRSV 8
           +ISP RS+
Sbjct: 277 SISPMRSI 284
>NP_115887
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NISPFRSVG 9
           N+ PF +VG
Sbjct: 200 NMLPFMAVG 208
>NP_066553
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISPFRSVG 9
           +SPFR  G
Sbjct: 129 LSPFRKQG 136
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.139    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 598,728
Number of extensions: 682
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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