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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           18    1482
NP_000240                                                              18    1482
NP_001161089                                                           18    1482
XP_005265218                                                           18    1482
NP_001192222                                                           16    5631
NP_001192223                                                           16    5631
NP_000712                                                              16    5631
NP_001258978                                                           16    7355
NP_057376                                                              16    7355
NP_001165349                                                           16    7355
NP_001165350                                                           16    7355
NP_005360                                                              16    7355
NP_001165347                                                           16    7355
NP_001258629                                                           16    7355
XP_006724820                                                           16    7355
XP_005262471                                                           16    7355
XP_005262470                                                           16    7355
NP_001165348                                                           16    7355
NP_055488                                                              16    7355
NP_001093325                                                           16    7355
NP_001008895                                                           15    9605
NP_001265442                                                           15    9605
NP_001265443                                                           15    9605
NP_003580                                                              15    9605
NP_001096078                                                           15    9605
XP_005247178                                                           15    9605

>NP_001245200
          Length = 687

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   NIRSIFG 7
           NIRSIFG
Sbjct: 215 NIRSIFG 221



>NP_000240
          Length = 756

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   NIRSIFG 7
           NIRSIFG
Sbjct: 215 NIRSIFG 221



>NP_001161089
          Length = 658

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   NIRSIFG 7
           NIRSIFG
Sbjct: 117 NIRSIFG 123



>XP_005265218
          Length = 687

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   NIRSIFG 7
           NIRSIFG
Sbjct: 215 NIRSIFG 221



>NP_001192222
          Length = 2313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NIRSIFGML 9
            N RS FG L
Sbjct: 1645 NFRSFFGSL 1653



>NP_001192223
          Length = 2251

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NIRSIFGML 9
            N RS FG L
Sbjct: 1626 NFRSFFGSL 1634



>NP_000712
          Length = 2270

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    NIRSIFGML 9
            N RS FG L
Sbjct: 1645 NFRSFFGSL 1653



>NP_001258978
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 285 NIRSIF 290



>NP_057376
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 338 NIRSIF 343



>NP_001165349
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 409 NIRSIF 414



>NP_001165350
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 409 NIRSIF 414



>NP_005360
          Length = 925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 409 NIRSIF 414



>NP_001165347
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 469 NIRSIF 474



>NP_001258629
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   NIRSIFG 7
           NIR+I+G
Sbjct: 383 NIRNIYG 389



>XP_006724820
          Length = 902

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 370 NIRSIF 375



>XP_005262471
          Length = 1005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 554 NIRSIF 559



>XP_005262470
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 512 NIRSIF 517



>NP_001165348
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 370 NIRSIF 375



>NP_055488
          Length = 297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   NIRSIFGM 8
           N+R++FG+
Sbjct: 225 NMRALFGL 232



>NP_001093325
          Length = 985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NIRSIF 6
           NIRSIF
Sbjct: 469 NIRSIF 474



>NP_001008895
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRSIFGML 9
           +RS+ GML
Sbjct: 214 LRSLLGML 221



>NP_001265442
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRSIFGML 9
           +RS+ GML
Sbjct: 114 LRSLLGML 121



>NP_001265443
          Length = 667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRSIFGML 9
           +RS+ GML
Sbjct: 122 LRSLLGML 129



>NP_003580
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IRSIFGML 9
           +RS+ GML
Sbjct: 114 LRSLLGML 121



>NP_001096078
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NIRSIFG 7
            NI+ IFG
Sbjct: 1069 NIKQIFG 1075



>XP_005247178
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    NIRSIFG 7
            NI+ IFG
Sbjct: 1069 NIKQIFG 1075



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.156    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 374,188
Number of extensions: 576
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)