Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              19     665
XP_006714104                                                           19     665
XP_005265800                                                           19     665
XP_006714103                                                           19     665
XP_006714102                                                           19     665
NP_006195                                                              16    7355
NP_001028752                                                           16    7355
NP_079385                                                              16    7355
NP_001157207                                                           16    7355
XP_006725415                                                           16    7355
XP_006725414                                                           16    7355
XP_006722171                                                           16    7355
XP_006722170                                                           16    7355
NP_997249                                                              15    9605
XP_005276506                                                           15    9605
XP_006726440                                                           15    9605
XP_006720412                                                           15    9605
XP_006711917                                                           15    9605
>NP_006197
          Length = 1089

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFYALKATS 9
           N YALKATS
Sbjct: 204 NVYALKATS 212
>XP_006714104
          Length = 832

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFYALKATS 9
           N YALKATS
Sbjct: 229 NVYALKATS 237
>XP_005265800
          Length = 1089

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFYALKATS 9
           N YALKATS
Sbjct: 204 NVYALKATS 212
>XP_006714103
          Length = 1111

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFYALKATS 9
           N YALKATS
Sbjct: 226 NVYALKATS 234
>XP_006714102
          Length = 1114

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NFYALKATS 9
           N YALKATS
Sbjct: 229 NVYALKATS 237
>NP_006195
          Length = 858

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFYALKATS 9
           N Y +K+TS
Sbjct: 611 NLYQMKSTS 619
>NP_001028752
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>NP_079385
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>NP_001157207
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>XP_006725415
          Length = 990

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>XP_006725414
          Length = 1034

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>XP_006722171
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>XP_006722170
          Length = 1016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           FYA+ ATS
Sbjct: 148 FYAVVATS 155
>NP_997249
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFYALKATS 9
           +FY +KA S
Sbjct: 405 DFYKMKAAS 413
>XP_005276506
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           +YA +ATS
Sbjct: 131 YYAARATS 138
>XP_006726440
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFYALKATS 9
           +FY +KA S
Sbjct: 98  DFYKMKAAS 106
>XP_006720412
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FYALKATS 9
           +YA +ATS
Sbjct: 108 YYAARATS 115
>XP_006711917
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   NFYALKATS 9
           +FY +KA S
Sbjct: 98  DFYKMKAAS 106
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.122    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 328,567
Number of extensions: 471
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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