Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              19     869
NP_115518                                                              16    5631
XP_006722394                                                           16    5631
XP_005258167                                                           16    5631
XP_006722393                                                           16    5631
XP_006722392                                                           16    5631
XP_006722391                                                           16    5631
XP_006722390                                                           16    5631
XP_005258166                                                           16    5631
XP_006722389                                                           16    5631
XP_005258165                                                           16    5631
XP_005258164                                                           16    5631
NP_054722                                                              16    7355
NP_079113                                                              16    7355
XP_005256326                                                           16    7355
XP_006720759                                                           16    7355
XP_006720758                                                           16    7355
XP_006720757                                                           16    7355
XP_006720756                                                           16    7355
NP_001182058                                                           15    9605
NP_071349                                                              15    9605
NP_001269539                                                           15    9605
NP_001269535                                                           15    9605
NP_057140                                                              15    9605
NP_001269538                                                           15    9605
NP_653292                                                              15    9605
NP_001161707                                                           15    9605
XP_006723846                                                           15    9605
XP_005257625                                                           15    9605
XP_005257624                                                           15    9605
XP_006710602                                                           15    9605
XP_006710601                                                           15    9605
XP_006710598                                                           15    9605
XP_006710597                                                           15    9605
XP_006710596                                                           15    9605
XP_006710595                                                           15    9605
XP_006710594                                                           15    9605
XP_006710593                                                           15    9605
XP_006710592                                                           15    9605
XP_006710591                                                           15    9605
XP_006710590                                                           15    9605
XP_006710589                                                           15    9605
XP_006710588                                                           15    9605
XP_006710587                                                           15    9605
XP_006710586                                                           15    9605
XP_006710585                                                           15    9605
XP_005270755                                                           15    9605
XP_006710584                                                           15    9605
XP_005270754                                                           15    9605
XP_005270753                                                           15    9605
XP_006710583                                                           15    9605
XP_005270752                                                           15    9605
XP_006710582                                                           15    9605
XP_005270751                                                           15    9605
NP_001157903                                                           15    9605
>NP_055365
          Length = 1032

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   NELLTTLTS 9
           +ELLTTLTS
Sbjct: 431 DELLTTLTS 439
>NP_115518
          Length = 2537

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2236 ELLTRLTS 2243
>XP_006722394
          Length = 1845

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 1543 ELLTRLTS 1550
>XP_005258167
          Length = 1856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 1554 ELLTRLTS 1561
>XP_006722393
          Length = 2199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 1897 ELLTRLTS 1904
>XP_006722392
          Length = 2444

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2142 ELLTRLTS 2149
>XP_006722391
          Length = 2448

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2146 ELLTRLTS 2153
>XP_006722390
          Length = 2467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2166 ELLTRLTS 2173
>XP_005258166
          Length = 2468

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2166 ELLTRLTS 2173
>XP_006722389
          Length = 2506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2236 ELLTRLTS 2243
>XP_005258165
          Length = 2534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2232 ELLTRLTS 2239
>XP_005258164
          Length = 2538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            ELLT LTS
Sbjct: 2236 ELLTRLTS 2243
>NP_054722
          Length = 1227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NELLTT 6
           NELLTT
Sbjct: 876 NELLTT 881
>NP_079113
          Length = 1192

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NELLTTLTS 9
           NE+L +LTS
Sbjct: 574 NEVLLSLTS 582
>XP_005256326
          Length = 1192

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   NELLTT 6
           NELLTT
Sbjct: 841 NELLTT 846
>XP_006720759
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NELLTTLTS 9
           NE+L +LTS
Sbjct: 611 NEVLLSLTS 619
>XP_006720758
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NELLTTLTS 9
           NE+L +LTS
Sbjct: 611 NEVLLSLTS 619
>XP_006720757
          Length = 1008

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NELLTTLTS 9
           NE+L +LTS
Sbjct: 390 NEVLLSLTS 398
>XP_006720756
          Length = 1229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NELLTTLTS 9
           NE+L +LTS
Sbjct: 611 NEVLLSLTS 619
>NP_001182058
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NELLTTLT 8
           NELL T+T
Sbjct: 152 NELLWTVT 159
>NP_071349
          Length = 1292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELLTTLTS 9
           E++TT+TS
Sbjct: 547 EVVTTMTS 554
>NP_001269539
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LLTTLTS 9
          LLTT+TS
Sbjct: 16 LLTTVTS 22
>NP_001269535
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLTTLTS 9
           LLTT+TS
Sbjct: 94  LLTTVTS 100
>NP_057140
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LLTTLTS 9
          LLTT+TS
Sbjct: 47 LLTTVTS 53
>NP_001269538
          Length = 162

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LLTTLTS 9
          LLTT+TS
Sbjct: 47 LLTTVTS 53
>NP_653292
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   NELLTTLTS 9
           N+L  TLTS
Sbjct: 658 NQLTQTLTS 666
>NP_001161707
          Length = 2139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    NELLTTLTS 9
            +ELL T+TS
Sbjct: 1103 DELLCTVTS 1111
>XP_006723846
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ELLTTLT 8
           ELL+TLT
Sbjct: 141 ELLSTLT 147
>XP_005257625
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELLTTLTS 9
           E++TT+TS
Sbjct: 522 EVVTTMTS 529
>XP_005257624
          Length = 1293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ELLTTLTS 9
           E++TT+TS
Sbjct: 547 EVVTTMTS 554
>XP_006710602
          Length = 7391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1992 ELMETLTS 1999
>XP_006710601
          Length = 4720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710598
          Length = 7553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1982 ELMETLTS 1989
>XP_006710597
          Length = 7451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1992 ELMETLTS 1999
>XP_006710596
          Length = 7512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1941 ELMETLTS 1948
>XP_006710595
          Length = 7526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1955 ELMETLTS 1962
>XP_006710594
          Length = 7528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1957 ELMETLTS 1964
>XP_006710593
          Length = 7539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1968 ELMETLTS 1975
>XP_006710592
          Length = 7544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1973 ELMETLTS 1980
>XP_006710591
          Length = 7558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1987 ELMETLTS 1994
>XP_006710590
          Length = 7563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 1992 ELMETLTS 1999
>XP_006710589
          Length = 7595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2024 ELMETLTS 2031
>XP_006710588
          Length = 7614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710587
          Length = 7645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710586
          Length = 7645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710585
          Length = 7657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_005270755
          Length = 7663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710584
          Length = 7686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_005270754
          Length = 7694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_005270753
          Length = 7700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710583
          Length = 7702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_005270752
          Length = 7717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_006710582
          Length = 7720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>XP_005270751
          Length = 7723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELLTTLTS 9
            EL+ TLTS
Sbjct: 2152 ELMETLTS 2159
>NP_001157903
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ELLTTLT 8
           ELL+TLT
Sbjct: 140 ELLSTLT 146
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.115    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,124
Number of extensions: 487
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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