Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           18    1935
NP_055903                                                              18    1935
NP_665698                                                              17    3301
NP_001098983                                                           17    3301
XP_006713075                                                           17    3301
XP_005264984                                                           17    3301
XP_005264982                                                           17    3301
XP_005264980                                                           17    3301
XP_006713074                                                           17    3301
XP_005264979                                                           17    3301
XP_005264978                                                           17    3301
NP_000366                                                              16    5631
NP_689737                                                              16    5631
NP_001153518                                                           16    5631
NP_001153505                                                           16    5631
XP_005270198                                                           16    5631
XP_005270197                                                           16    5631
XP_005270196                                                           16    5631
XP_005270195                                                           16    5631
XP_006718023                                                           16    5631
XP_005270194                                                           16    5631
XP_005246402                                                           16    5631
XP_006712393                                                           16    5631
XP_005246401                                                           16    5631
>NP_001155973
          Length = 1833

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLASKVG 9
           NASLASK G
Sbjct: 788 NASLASKGG 796
>NP_055903
          Length = 1723

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   NASLASKVG 9
           NASLASK G
Sbjct: 788 NASLASKGG 796
>NP_665698
          Length = 1320

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 761 DASLASRIG 769
>NP_001098983
          Length = 1272

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 713 DASLASRIG 721
>XP_006713075
          Length = 1019

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 754 DASLASRIG 762
>XP_005264984
          Length = 1140

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 567 DASLASRIG 575
>XP_005264982
          Length = 1286

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 713 DASLASRIG 721
>XP_005264980
          Length = 1313

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 754 DASLASRIG 762
>XP_006713074
          Length = 1318

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 754 DASLASRIG 762
>XP_005264979
          Length = 1322

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 754 DASLASRIG 762
>XP_005264978
          Length = 1327

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NASLASKVG 9
           +ASLAS++G
Sbjct: 754 DASLASRIG 762
>NP_000366
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>NP_689737
          Length = 1265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 32 NVSLASKI 39
>NP_001153518
          Length = 1261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 32 NVSLASKI 39
>NP_001153505
          Length = 1260

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 32 NVSLASKI 39
>XP_005270198
          Length = 139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_005270197
          Length = 223

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_005270196
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_005270195
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_006718023
          Length = 292

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_005270194
          Length = 292

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ASLASKVG 9
           ASL SK+G
Sbjct: 104 ASLVSKIG 111
>XP_005246402
          Length = 1265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 32 NVSLASKI 39
>XP_006712393
          Length = 1267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 38 NVSLASKI 45
>XP_005246401
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  NASLASKV 8
          N SLASK+
Sbjct: 38 NVSLASKI 45
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.110    0.259 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 227,795
Number of extensions: 314
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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