Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     299
NP_671714                                                              20     299
XP_006715890                                                           20     299
XP_005250160                                                           20     299
XP_005250159                                                           20     299
NP_001171922                                                           18    1935
XP_006719916                                                           18    1935
XP_006719915                                                           18    1935
XP_006719914                                                           18    1935
XP_005266530                                                           18    1935
XP_006719913                                                           18    1935
XP_006719912                                                           18    1935
XP_006719911                                                           18    1935
XP_006719910                                                           18    1935
XP_006719909                                                           18    1935
NP_112567                                                              18    1935
NP_001230175                                                           17    4312
NP_071751                                                              17    4312
NP_001139761                                                           17    4312
NP_001274324                                                           17    4312
XP_006712547                                                           17    4312
NP_003099                                                              16    5631
NP_653250                                                              16    5631
XP_006716571                                                           16    5631
XP_006716570                                                           16    5631
XP_005250852                                                           16    5631
XP_005250851                                                           16    5631
NP_689755                                                              16    7355
NP_001123361                                                           16    7355
NP_001913                                                              16    7355
XP_006714215                                                           16    7355
XP_006714214                                                           16    7355
XP_005262915                                                           16    7355
XP_006714174                                                           16    7355
NP_055806                                                              16    7355
NP_055731                                                              15    9605
NP_001128625                                                           15    9605
NP_036553                                                              15    9605
NP_001036037                                                           15    9605
NP_001262                                                              15    9605
XP_005273523                                                           15    9605
XP_006716387                                                           15    9605
XP_005273522                                                           15    9605
XP_005273521                                                           15    9605
XP_005273520                                                           15    9605
XP_006716386                                                           15    9605
NP_001121903                                                           15    9605
NP_001128626                                                           15    9605
NP_056174                                                              15    9605
>NP_005742
          Length = 3907

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            NAHSLSE+A
Sbjct: 2373 NAHSLSEEA 2381
>NP_671714
          Length = 3899

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            NAHSLSE+A
Sbjct: 2365 NAHSLSEEA 2373
>XP_006715890
          Length = 3860

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            NAHSLSE+A
Sbjct: 2326 NAHSLSEEA 2334
>XP_005250160
          Length = 3939

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            NAHSLSE+A
Sbjct: 2405 NAHSLSEEA 2413
>XP_005250159
          Length = 3952

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            NAHSLSE+A
Sbjct: 2418 NAHSLSEEA 2426
>NP_001171922
          Length = 1619

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1109 NSHSLSEKS 1117
>XP_006719916
          Length = 1482

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   NAHSLSEQA 9
           N+HSLSE++
Sbjct: 972 NSHSLSEKS 980
>XP_006719915
          Length = 1531

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1021 NSHSLSEKS 1029
>XP_006719914
          Length = 1639

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1129 NSHSLSEKS 1137
>XP_005266530
          Length = 1643

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1133 NSHSLSEKS 1141
>XP_006719913
          Length = 1650

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1182 NSHSLSEKS 1190
>XP_006719912
          Length = 1672

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1162 NSHSLSEKS 1170
>XP_006719911
          Length = 1688

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1178 NSHSLSEKS 1186
>XP_006719910
          Length = 1692

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1182 NSHSLSEKS 1190
>XP_006719909
          Length = 1692

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1182 NSHSLSEKS 1190
>NP_112567
          Length = 1623

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    NAHSLSEQA 9
            N+HSLSE++
Sbjct: 1113 NSHSLSEKS 1121
>NP_001230175
          Length = 804

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AHSLSEQ 8
           AHSLSE+
Sbjct: 129 AHSLSEE 135
>NP_071751
          Length = 736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AHSLSEQ 8
           AHSLSE+
Sbjct: 129 AHSLSEE 135
>NP_001139761
          Length = 697

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AHSLSEQ 8
           AHSLSE+
Sbjct: 129 AHSLSEE 135
>NP_001274324
          Length = 1688

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AHSLSEQA 9
            AH+L EQA
Sbjct: 1316 AHTLDEQA 1323
>XP_006712547
          Length = 1674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AHSLSEQA 9
            AH+L EQA
Sbjct: 1308 AHTLDEQA 1315
>NP_003099
          Length = 441

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   NAHSLSEQ 8
           +AHS SEQ
Sbjct: 372 SAHSASEQ 379
>NP_653250
          Length = 276

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NAHSLSEQA 9
           N+HSLS Q+
Sbjct: 182 NSHSLSLQS 190
>XP_006716571
          Length = 116

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  NAHSLSEQA 9
          N+HSLS Q+
Sbjct: 22 NSHSLSLQS 30
>XP_006716570
          Length = 290

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NAHSLSEQA 9
           N+HSLS Q+
Sbjct: 196 NSHSLSLQS 204
>XP_005250852
          Length = 294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NAHSLSEQA 9
           N+HSLS Q+
Sbjct: 200 NSHSLSLQS 208
>XP_005250851
          Length = 295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   NAHSLSEQA 9
           N+HSLS Q+
Sbjct: 201 NSHSLSLQS 209
>NP_689755
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AHSLSEQA 9
           AH+++EQA
Sbjct: 323 AHAVTEQA 330
>NP_001123361
          Length = 552

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NAHSLSEQ 8
           N HSLS Q
Sbjct: 129 NIHSLSPQ 136
>NP_001913
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   NAHSLSEQ 8
           N HSLS Q
Sbjct: 129 NIHSLSPQ 136
>XP_006714215
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    NAHSLSEQ 8
            N HSLS Q
Sbjct: 2316 NKHSLSTQ 2323
>XP_006714214
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    NAHSLSEQ 8
            N HSLS Q
Sbjct: 2316 NKHSLSTQ 2323
>XP_005262915
          Length = 3544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    NAHSLSEQ 8
            N HSLS Q
Sbjct: 2316 NKHSLSTQ 2323
>XP_006714174
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AHSLSEQA 9
           AH+++EQA
Sbjct: 323 AHAVTEQA 330
>NP_055806
          Length = 3526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    NAHSLSEQ 8
            N HSLS Q
Sbjct: 2298 NKHSLSTQ 2305
>NP_055731
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    NAHSLSEQA 9
            N HSLS ++
Sbjct: 1302 NLHSLSSES 1310
>NP_001128625
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>NP_036553
          Length = 1914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HSLSEQA 9
           HSL++QA
Sbjct: 641 HSLNDQA 647
>NP_001036037
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NAHSLSEQA 9
          ++HS SE+A
Sbjct: 20 SSHSASEEA 28
>NP_001262
          Length = 1828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  NAHSLSEQA 9
          ++HS SE+A
Sbjct: 20 SSHSASEEA 28
>XP_005273523
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>XP_006716387
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>XP_005273522
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>XP_005273521
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 110 AHNFSEQS 117
>XP_005273520
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 110 AHNFSEQS 117
>XP_006716386
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 110 AHNFSEQS 117
>NP_001121903
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>NP_001128626
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
>NP_056174
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AHSLSEQA 9
           AH+ SEQ+
Sbjct: 100 AHNFSEQS 107
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290   0.0989    0.239 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 554,866
Number of extensions: 879
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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