Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              20     299
XP_006714104                                                           20     299
XP_005265800                                                           20     299
XP_006714103                                                           20     299
XP_006714102                                                           20     299
NP_653248                                                              17    4312
NP_001036090                                                           17    4312
NP_065928                                                              17    4312
XP_005256527                                                           17    4312
XP_006714508                                                           17    4312
XP_006714507                                                           17    4312
NP_008825                                                              16    5631
NP_001002294                                                           16    5631
NP_000821                                                              16    5631
NP_783300                                                              16    5631
NP_001073864                                                           16    5631
NP_001074003                                                           16    5631
NP_004911                                                              16    5631
XP_005261001                                                           16    5631
XP_005261000                                                           16    5631
XP_005260999                                                           16    5631
XP_006724054                                                           16    5631
XP_005257912                                                           16    5631
XP_006722259                                                           16    5631
XP_006722258                                                           16    5631
XP_006722257                                                           16    5631
XP_006722256                                                           16    5631
XP_005254452                                                           16    5631
XP_005254451                                                           16    5631
XP_005245101                                                           16    5631
NP_006243                                                              15    9605
NP_057107                                                              15    9605

>NP_006197
          Length = 1089

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MTDYLVIME 9
           MTDYLVI+E
Sbjct: 133 MTDYLVIVE 141



>XP_006714104
          Length = 832

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MTDYLVIME 9
           MTDYLVI+E
Sbjct: 158 MTDYLVIVE 166



>XP_005265800
          Length = 1089

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MTDYLVIME 9
           MTDYLVI+E
Sbjct: 133 MTDYLVIVE 141



>XP_006714103
          Length = 1111

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MTDYLVIME 9
           MTDYLVI+E
Sbjct: 155 MTDYLVIVE 163



>XP_006714102
          Length = 1114

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MTDYLVIME 9
           MTDYLVI+E
Sbjct: 158 MTDYLVIVE 166



>NP_653248
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MTDYLVIME 9
          + DY V+ME
Sbjct: 68 LNDYAVVME 76



>NP_001036090
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MTDYLVIME 9
          + DY V+ME
Sbjct: 68 LNDYAVVME 76



>NP_065928
          Length = 4427

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MTDYLVIME 9
            M+DYL I+E
Sbjct: 3421 MSDYLRILE 3429



>XP_005256527
          Length = 4427

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    MTDYLVIME 9
            M+DYL I+E
Sbjct: 3421 MSDYLRILE 3429



>XP_006714508
          Length = 199

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MTDYLVIME 9
          + DY V+ME
Sbjct: 85 LNDYAVVME 93



>XP_006714507
          Length = 225

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MTDYLVIME 9
          + DY V+ME
Sbjct: 85 LNDYAVVME 93



>NP_008825
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+V M+
Sbjct: 428 TDYIVYMD 435



>NP_001002294
          Length = 532

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+V M+
Sbjct: 428 TDYIVYMD 435



>NP_000821
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 746 TDYALLME 753



>NP_783300
          Length = 905

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 731 TDYALLME 738



>NP_001073864
          Length = 1374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 195 VTPYLLVME 203



>NP_001074003
          Length = 2214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+ +ME
Sbjct: 876 TDYVEVME 883



>NP_004911
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 92  VTPYLLVME 100



>XP_005261001
          Length = 903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 731 TDYALLME 738



>XP_005261000
          Length = 920

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 746 TDYALLME 753



>XP_005260999
          Length = 934

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 731 TDYALLME 738



>XP_006724054
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY ++ME
Sbjct: 746 TDYALLME 753



>XP_005257912
          Length = 915

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 195 VTPYLLVME 203



>XP_006722259
          Length = 1326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 147 VTPYLLVME 155



>XP_006722258
          Length = 1207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  MTDYLVIME 9
          +T YL++ME
Sbjct: 28 VTPYLLVME 36



>XP_006722257
          Length = 1324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 195 VTPYLLVME 203



>XP_006722256
          Length = 1326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   MTDYLVIME 9
           +T YL++ME
Sbjct: 147 VTPYLLVME 155



>XP_005254452
          Length = 2188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+ +ME
Sbjct: 876 TDYVEVME 883



>XP_005254451
          Length = 2214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+ +ME
Sbjct: 876 TDYVEVME 883



>XP_005245101
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TDYLVIME 9
           TDY+V M+
Sbjct: 365 TDYIVYMD 372



>NP_006243
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  TDYLVIME 9
          TD+ ++ME
Sbjct: 87 TDFFMVME 94



>NP_057107
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  YLVIME 9
          YLV+ME
Sbjct: 27 YLVVME 32



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.143    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 426,285
Number of extensions: 495
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)