Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     390
NP_005913                                                              20     390
XP_005267046                                                           20     390
XP_005267045                                                           20     390
NP_055723                                                              16    5631
NP_068761                                                              16    5631
NP_951008                                                              16    5631
NP_777576                                                              16    7355
NP_005391                                                              15    9605
NP_001229342                                                           15    9605
NP_000563                                                              15    9605
NP_004806                                                              15    9605
NP_612210                                                              15    9605
NP_963917                                                              15    9605
NP_001193939                                                           15    9605
NP_006248                                                              15    9605
NP_001269574                                                           15    9605
NP_001269573                                                           15    9605
NP_002949                                                              15    9605
NP_001073918                                                           15    9605
XP_006719100                                                           15    9605
XP_006719099                                                           15    9605
XP_006719098                                                           15    9605
XP_006719097                                                           15    9605
XP_005253381                                                           15    9605
XP_006719096                                                           15    9605
XP_006717743                                                           15    9605
XP_005252554                                                           15    9605
XP_006717528                                                           15    9605
XP_005252553                                                           15    9605
XP_006712113                                                           15    9605
XP_006712112                                                           15    9605
XP_005264486                                                           15    9605
XP_005264485                                                           15    9605
XP_006711111                                                           15    9605
NP_001005861                                                           15    9605
>NP_006715
          Length = 1558

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MQVDNILLV 9
           MQVDN+LLV
Sbjct: 940 MQVDNLLLV 948
>NP_005913
          Length = 1608

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MQVDNILLV 9
           MQVDN+LLV
Sbjct: 940 MQVDNLLLV 948
>XP_005267046
          Length = 1554

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MQVDNILLV 9
           MQVDN+LLV
Sbjct: 940 MQVDNLLLV 948
>XP_005267045
          Length = 1604

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MQVDNILLV 9
           MQVDN+LLV
Sbjct: 940 MQVDNLLLV 948
>NP_055723
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QVDNILL 8
           Q+DN+LL
Sbjct: 522 QIDNLLL 528
>NP_068761
          Length = 1133

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QVDNILL 8
           QVDN++L
Sbjct: 947 QVDNVIL 953
>NP_951008
          Length = 1086

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QVDNILL 8
           QVDN++L
Sbjct: 947 QVDNVIL 953
>NP_777576
          Length = 1749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MQVDNILLV 9
           MQ+ NILL+
Sbjct: 540 MQLKNILLM 548
>NP_005391
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 533 LKLDNILL 540
>NP_001229342
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 505 LKLDNILL 512
>NP_000563
          Length = 178

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  QVDNILL 8
          Q+DN+LL
Sbjct: 60 QLDNLLL 66
>NP_004806
          Length = 1261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DN+LL
Sbjct: 192 LELDNVLL 199
>NP_612210
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 203 MRVDNII 209
>NP_963917
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 170 MRVDNII 176
>NP_001193939
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 213 MRVDNII 219
>NP_006248
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 505 LKLDNILL 512
>NP_001269574
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 380 LKLDNILL 387
>NP_001269573
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 469 LKLDNILL 476
>NP_002949
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QVDNIL 7
           QVDN+L
Sbjct: 126 QVDNVL 131
>NP_001073918
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDNILL 8
           VDNILL
Sbjct: 682 VDNILL 687
>XP_006719100
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 203 MRVDNII 209
>XP_006719099
          Length = 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 220 MRVDNII 226
>XP_006719098
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 220 MRVDNII 226
>XP_006719097
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 203 MRVDNII 209
>XP_005253381
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 203 MRVDNII 209
>XP_006719096
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MQVDNIL 7
           M+VDNI+
Sbjct: 220 MRVDNII 226
>XP_006717743
          Length = 1090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   VDNILL 8
           VDNILL
Sbjct: 712 VDNILL 717
>XP_005252554
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 539 LKLDNILL 546
>XP_006717528
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 505 LKLDNILL 512
>XP_005252553
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 539 LKLDNILL 546
>XP_006712113
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 256 LKLDNILL 263
>XP_006712112
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 383 LKLDNILL 390
>XP_005264486
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 396 LKLDNILL 403
>XP_005264485
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DNILL
Sbjct: 533 LKLDNILL 540
>XP_006711111
          Length = 1197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   MQVDNILL 8
           +++DN+LL
Sbjct: 128 LELDNVLL 135
>NP_001005861
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QVDNIL 7
           QVDN+L
Sbjct: 126 QVDNVL 131
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.149    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 394,663
Number of extensions: 522
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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