Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           20     509
NP_036462                                                              20     509
NP_001243397                                                           20     509
XP_005269721                                                           20     509
NP_001092882                                                           18    1482
NP_006757                                                              18    1482
NP_001092883                                                           18    1482
XP_005273706                                                           18    1482
NP_004766                                                              16    5631
XP_006722642                                                           16    5631
XP_006722641                                                           16    5631
XP_005258444                                                           16    5631
NP_872363                                                              16    7355
XP_005271570                                                           16    7355
XP_005271569                                                           16    7355
XP_006718878                                                           16    7355
XP_005271568                                                           16    7355
NP_115529                                                              15    9605
NP_001161851                                                           15    9605
NP_001161850                                                           15    9605
>NP_001243398
          Length = 1781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    MNMSVNLM 8
            MNMSVNLM
Sbjct: 1673 MNMSVNLM 1680
>NP_036462
          Length = 2073

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    MNMSVNLM 8
            MNMSVNLM
Sbjct: 1965 MNMSVNLM 1972
>NP_001243397
          Length = 1890

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    MNMSVNLM 8
            MNMSVNLM
Sbjct: 1782 MNMSVNLM 1789
>XP_005269721
          Length = 2073

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    MNMSVNLM 8
            MNMSVNLM
Sbjct: 1965 MNMSVNLM 1972
>NP_001092882
          Length = 2004

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    MNMSVNLM 8
            MNM VNLM
Sbjct: 1897 MNMGVNLM 1904
>NP_006757
          Length = 2004

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    MNMSVNLM 8
            MNM VNLM
Sbjct: 1897 MNMGVNLM 1904
>NP_001092883
          Length = 2004

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    MNMSVNLM 8
            MNM VNLM
Sbjct: 1897 MNMGVNLM 1904
>XP_005273706
          Length = 1997

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    MNMSVNLM 8
            MNM VNLM
Sbjct: 1890 MNMGVNLM 1897
>NP_004766
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NMSVNLM 8
           N+SVNLM
Sbjct: 367 NISVNLM 373
>XP_006722642
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NMSVNLM 8
           N+SVNLM
Sbjct: 323 NISVNLM 329
>XP_006722641
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NMSVNLM 8
           N+SVNLM
Sbjct: 329 NISVNLM 335
>XP_005258444
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NMSVNLM 8
           N+SVNLM
Sbjct: 355 NISVNLM 361
>NP_872363
          Length = 1499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM+VN+
Sbjct: 772 MNMNVNM 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM++NL
Sbjct: 778 MNMNMNL 784
>XP_005271570
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM+VN+
Sbjct: 735 MNMNVNM 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM++NL
Sbjct: 741 MNMNMNL 747
>XP_005271569
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM+VN+
Sbjct: 735 MNMNVNM 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM++NL
Sbjct: 741 MNMNMNL 747
>XP_006718878
          Length = 1499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM+VN+
Sbjct: 772 MNMNVNM 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM++NL
Sbjct: 778 MNMNMNL 784
>XP_005271568
          Length = 1499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM+VN+
Sbjct: 772 MNMNVNM 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MNM++NL
Sbjct: 778 MNMNMNL 784
>NP_115529
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MN++VNL
Sbjct: 101 MNLTVNL 107
>NP_001161851
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MN++VNL
Sbjct: 139 MNLTVNL 145
>NP_001161850
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MNMSVNL 7
           MN++VNL
Sbjct: 151 MNLTVNL 157
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.114    0.272 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 242,481
Number of extensions: 246
Number of successful extensions: 25
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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