Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     390
XP_005251512                                                           20     390
NP_071504                                                              17    2528
NP_000911                                                              17    2528
NP_001035806                                                           17    2528
XP_006718641                                                           17    2528
XP_005274089                                                           17    2528
XP_005274088                                                           17    2528
XP_006718640                                                           17    2528
NP_001138617                                                           16    7355
NP_005619                                                              16    7355
NP_001138616                                                           16    7355
XP_005259224                                                           16    7355
NP_003879                                                              15    9605
NP_001193826                                                           15    9605
NP_733797                                                              15    9605
NP_733798                                                              15    9605
NP_001265354                                                           15    9605
NP_002903                                                              15    9605
NP_001273361                                                           15    9605
NP_001273360                                                           15    9605
NP_000680                                                              15    9605
NP_694546                                                              15    9605
NP_006494                                                              15    9605
NP_000684                                                              15    9605
NP_001243323                                                           15    9605
NP_000683                                                              15    9605
NP_443124                                                              15    9605
NP_001180422                                                           15    9605
NP_003029                                                              15    9605
NP_060418                                                              15    9605
XP_006717236                                                           15    9605
XP_006717235                                                           15    9605
XP_006717234                                                           15    9605
XP_006717233                                                           15    9605
XP_006717232                                                           15    9605
XP_006717231                                                           15    9605
XP_006717230                                                           15    9605
XP_006717229                                                           15    9605
XP_005267148                                                           15    9605
XP_005267147                                                           15    9605
XP_006715607                                                           15    9605
XP_006715606                                                           15    9605
XP_006712142                                                           15    9605
>NP_004963
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           MMRIAKEN+
Sbjct: 186 MMRIAKEND 194
>XP_005251512
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           MMRIAKEN+
Sbjct: 186 MMRIAKEND 194
>NP_071504
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>NP_000911
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>NP_001035806
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>XP_006718641
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>XP_005274089
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>XP_005274088
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>XP_006718640
          Length = 1178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MMRIAKENN 9
           ++++AKENN
Sbjct: 102 IIKVAKENN 110
>NP_001138617
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 158 MMKCVEENN 166
>NP_005619
          Length = 541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 360 MMKCVEENN 368
>NP_001138616
          Length = 313

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 132 MMKCVEENN 140
>XP_005259224
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 360 MMKCVEENN 368
>NP_003879
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 411 MRIAKE 416
>NP_001193826
          Length = 497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 390 MRIAKE 395
>NP_733797
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 373 MRIAKE 378
>NP_733798
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 315 MRIAKE 320
>NP_001265354
          Length = 1774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RIAKENN 9
           R+AKE N
Sbjct: 936 RVAKEQN 942
>NP_002903
          Length = 3130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2567 MLRIAKPMN 2575
>NP_001273361
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2489 MLRIAKPMN 2497
>NP_001273360
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2489 MLRIAKPMN 2497
>NP_000680
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 394 MRIAKE 399
>NP_694546
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MMRIAKEN 8
           + R+AKEN
Sbjct: 219 VFRLAKEN 226
>NP_006494
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MMRIAKEN 8
           + R+AKEN
Sbjct: 250 VFRLAKEN 257
>NP_000684
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 405 MRIAKE 410
>NP_001243323
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 242 MMIAKEEN 249
>NP_000683
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MRIAKE 7
           MRIAKE
Sbjct: 410 MRIAKE 415
>NP_443124
          Length = 2459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    RIAKENN 9
            R+AKE N
Sbjct: 1621 RVAKEQN 1627
>NP_001180422
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  MMRIAKENN 9
          MM+  +ENN
Sbjct: 54 MMKCIEENN 62
>NP_003029
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 352 MMKCIEENN 360
>NP_060418
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 310 MMIAKEEN 317
>XP_006717236
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 184 MMIAKEEN 191
>XP_006717235
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 184 MMIAKEEN 191
>XP_006717234
          Length = 1041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 184 MMIAKEEN 191
>XP_006717233
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 242 MMIAKEEN 249
>XP_006717232
          Length = 1099

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 242 MMIAKEEN 249
>XP_006717231
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 310 MMIAKEEN 317
>XP_006717230
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 310 MMIAKEEN 317
>XP_006717229
          Length = 1167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   MRIAKENN 9
           M IAKE N
Sbjct: 310 MMIAKEEN 317
>XP_005267148
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2567 MLRIAKPMN 2575
>XP_005267147
          Length = 2689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2567 MLRIAKPMN 2575
>XP_006715607
          Length = 3052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2489 MLRIAKPMN 2497
>XP_006715606
          Length = 3130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    MMRIAKENN 9
            M+RIAK  N
Sbjct: 2567 MLRIAKPMN 2575
>XP_006712142
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   MMRIAKENN 9
           MM+  +ENN
Sbjct: 132 MMKCIEENN 140
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.122    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,416
Number of extensions: 792
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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