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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_006718908                                                           18    1135
XP_005271621                                                           18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_005920                                                              15    9605
NP_201573                                                              15    9605
XP_006713707                                                           15    9605
XP_006713706                                                           15    9605
XP_006713705                                                           15    9605

>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 2223 IMALRTVIL 2231



>XP_006718908
          Length = 1708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   MMALRTVIL 9
           +MALRTVIL
Sbjct: 875 IMALRTVIL 883



>XP_005271621
          Length = 2708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 1875 IMALRTVIL 1883



>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 2217 IMALRTVIL 2225



>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 2223 IMALRTVIL 2231



>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 2223 IMALRTVIL 2231



>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MMALRTVIL 9
            +MALRTVIL
Sbjct: 2223 IMALRTVIL 2231



>NP_005920
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MMALRTVI 8
          ++ALRTV+
Sbjct: 11 LLALRTVL 18



>NP_201573
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MMALRTVI 8
          ++ALRTV+
Sbjct: 11 LLALRTVL 18



>XP_006713707
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MMALRTVI 8
          ++ALRTV+
Sbjct: 11 LLALRTVL 18



>XP_006713706
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MMALRTVI 8
          ++ALRTV+
Sbjct: 11 LLALRTVL 18



>XP_006713705
          Length = 760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  MMALRTVI 8
          ++ALRTV+
Sbjct: 11 LLALRTVL 18



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.355    0.149    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 223,962
Number of extensions: 180
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.1 bits)
S2: 28 (15.4 bits)