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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_001012989                                                           16    7355
NP_006036                                                              16    7355
NP_060440                                                              16    7355
NP_001013859                                                           15    9605
NP_001013858                                                           15    9605
NP_003541                                                              15    9605
NP_061973                                                              15    9605
NP_001029195                                                           15    9605
NP_001029196                                                           15    9605
NP_001185731                                                           15    9605
NP_001185730                                                           15    9605
NP_004757                                                              15    9605
NP_003751                                                              15    9605
NP_942147                                                              15    9605
XP_005259228                                                           15    9605
XP_005259227                                                           15    9605
XP_005259226                                                           15    9605
XP_005249935                                                           15    9605
XP_005249934                                                           15    9605
XP_006715851                                                           15    9605
XP_005249933                                                           15    9605
XP_006710835                                                           15    9605
XP_006710834                                                           15    9605
XP_005271096                                                           15    9605
XP_005271097                                                           15    9605
XP_005271094                                                           15    9605

>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLEMEVELS 9
            MLEM+VELS
Sbjct: 3133 MLEMQVELS 3141



>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLEMEVELS 9
            MLEM+VELS
Sbjct: 3125 MLEMQVELS 3133



>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLEMEVELS 9
            MLEM+VELS
Sbjct: 3086 MLEMQVELS 3094



>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLEMEVELS 9
            MLEM+VELS
Sbjct: 3165 MLEMQVELS 3173



>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MLEMEVELS 9
            MLEM+VELS
Sbjct: 3178 MLEMQVELS 3186



>NP_001012989
          Length = 171

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           ++E+EVEL
Sbjct: 116 LMELEVEL 123



>NP_006036
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEMEVEL 8
           LEME+EL
Sbjct: 639 LEMEMEL 645



>NP_060440
          Length = 1164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EMEVELS 9
           E+EVELS
Sbjct: 829 ELEVELS 835



>NP_001013859
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_001013858
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_003541
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_061973
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



>NP_001029195
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



>NP_001029196
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



>NP_001185731
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLEMEVEL 8
           MLE E+EL
Sbjct: 528 MLEAELEL 535



>NP_001185730
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLEMEVEL 8
           MLE E+EL
Sbjct: 521 MLEAELEL 528



>NP_004757
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           +LE+EV+L
Sbjct: 877 LLELEVDL 884



>NP_003751
          Length = 1585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   MLEMEVEL 8
           MLE E+EL
Sbjct: 522 MLEAELEL 529



>NP_942147
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEMEVELS 9
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259228
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEMEVELS 9
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259227
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEMEVELS 9
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005259226
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEMEVELS 9
           +EMEVE++
Sbjct: 131 IEMEVEVA 138



>XP_005249935
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 425 MLEMELKM 432



>XP_005249934
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 425 MLEMELKM 432



>XP_006715851
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 472 MLEMELKM 479



>XP_005249933
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   MLEMEVEL 8
           MLEME+++
Sbjct: 472 MLEMELKM 479



>XP_006710835
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 294 LLELGVELS 302



>XP_006710834
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 297 LLELGVELS 305



>XP_005271096
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



>XP_005271097
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



>XP_005271094
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MLEMEVELS 9
           +LE+ VELS
Sbjct: 345 LLELGVELS 353



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.125    0.275 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 441,324
Number of extensions: 640
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)