Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_004963                                                              17    4312
XP_005251512                                                           17    4312
NP_878269                                                              16    5631
NP_878272                                                              16    5631
NP_067001                                                              16    5631
XP_006721506                                                           16    5631
XP_005256500                                                           16    5631
XP_006721505                                                           16    5631
XP_006721504                                                           16    5631
NP_998762                                                              16    5631
NP_001273913                                                           16    7355
NP_001107867                                                           16    7355
NP_060208                                                              16    7355
XP_005251549                                                           16    7355
NP_078963                                                              15    9605
NP_004384                                                              15    9605
NP_001171110                                                           15    9605
NP_001171113                                                           15    9605
NP_001171114                                                           15    9605
NP_001159400                                                           15    9605
NP_001171111                                                           15    9605
NP_001171112                                                           15    9605
NP_001171115                                                           15    9605
NP_001171107                                                           15    9605
NP_001171105                                                           15    9605
NP_001171106                                                           15    9605
NP_001171109                                                           15    9605
NP_001171108                                                           15    9605
NP_001186131                                                           15    9605
NP_006218                                                              15    9605
NP_872617                                                              15    9605
NP_001186130                                                           15    9605
NP_001229849                                                           15    9605
NP_001229850                                                           15    9605
XP_005260496                                                           15    9605
XP_006713811                                                           15    9605
XP_006713810                                                           15    9605
XP_005247834                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEVELSSMK 9
            M+VELSSMK
Sbjct: 3136 MQVELSSMK 3144
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEVELSSMK 9
            M+VELSSMK
Sbjct: 3128 MQVELSSMK 3136
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEVELSSMK 9
            M+VELSSMK
Sbjct: 3089 MQVELSSMK 3097
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEVELSSMK 9
            M+VELSSMK
Sbjct: 3168 MQVELSSMK 3176
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    MEVELSSMK 9
            M+VELSSMK
Sbjct: 3181 MQVELSSMK 3189
>NP_004963
          Length = 1132

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEVELSSMK 9
           +E+ELSS++
Sbjct: 352 LEIELSSLR 360
>XP_005251512
          Length = 1132

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEVELSSMK 9
           +E+ELSS++
Sbjct: 352 LEIELSSLR 360
>NP_878269
          Length = 277

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           +VEL+SMK
Sbjct: 133 DVELTSMK 140
>NP_878272
          Length = 277

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           +VEL+SMK
Sbjct: 133 DVELTSMK 140
>NP_067001
          Length = 277

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           +VEL+SMK
Sbjct: 133 DVELTSMK 140
>XP_006721506
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MEVELSSMK 9
          ME+E+S+M+
Sbjct: 19 MEMEISNMR 27
>XP_005256500
          Length = 458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  MEVELSSMK 9
          ME+E+S+M+
Sbjct: 67 MEMEISNMR 75
>XP_006721505
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEVELSSMK 9
           ME+E+S+M+
Sbjct: 101 MEMEISNMR 109
>XP_006721504
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEVELSSMK 9
           ME+E+S+M+
Sbjct: 101 MEMEISNMR 109
>NP_998762
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   MEVELSSMK 9
           ME+E+S+M+
Sbjct: 101 MEMEISNMR 109
>NP_001273913
          Length = 203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEVELSSMK 9
           +E ELSS+K
Sbjct: 105 LEEELSSLK 113
>NP_001107867
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEVELSSMK 9
           +E ELSS+K
Sbjct: 105 LEEELSSLK 113
>NP_060208
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEVELSSMK 9
           +E ELSS+K
Sbjct: 105 LEEELSSLK 113
>XP_005251549
          Length = 1405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MEVELSSMK 9
           +E ELSS+K
Sbjct: 105 LEEELSSLK 113
>NP_078963
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 229 EVEITTMK 236
>NP_004384
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171110
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171113
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171114
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001159400
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171111
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171112
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171115
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171107
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171105
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171106
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171109
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001171108
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EVELSSMK 9
           EVEL +MK
Sbjct: 219 EVELHNMK 226
>NP_001186131
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 200 EVEITTMK 207
>NP_006218
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVELSSM 8
           EV+LSSM
Sbjct: 361 EVQLSSM 367
>NP_872617
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVELSSM 8
           EV+LSSM
Sbjct: 309 EVQLSSM 315
>NP_001186130
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 229 EVEITTMK 236
>NP_001229849
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVELSSM 8
           EV+LSSM
Sbjct: 266 EVQLSSM 272
>NP_001229850
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVELSSM 8
           EV+LSSM
Sbjct: 273 EVQLSSM 279
>XP_005260496
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVELSSM 8
           EV+LSSM
Sbjct: 381 EVQLSSM 387
>XP_006713811
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 229 EVEITTMK 236
>XP_006713810
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 229 EVEITTMK 236
>XP_005247834
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVELSSMK 9
           EVE+++MK
Sbjct: 229 EVEITTMK 236
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.111    0.237 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 308,516
Number of extensions: 330
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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